| TFome pUT5751 | |
|---|---|
| Template | BT061983.1 |
| Genbank Insert ID | KJ727799 |
| Protein Name | ZmWRKY11 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G138683 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A393T p.R131S Not present in functional domain. Not present in template FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT5751
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B360_F |
| Temperature (ºC) | 67.4 |
| Sequence
|
caccATGTGCGACTACTTCCTACAAAGG |
3' Primer
| Name | B360_R |
| Temperature (ºC) | |
| Sequence
|
CCAGCCACTCTCGTCTTGCTCAA |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MCDYFLQRMEDDRHHHHQAGGDLTDVVRAGGAMHPAAVAELSSTATTGWQLPAEPAGPPGLFPPARSSSSSDGGAFAGLSGPFASDFRADSAGVDPSPADFFDFEAPAVGGGRGGMVEGGGPPQMPALSPSEVRPYSVMMGGDTVKIGVPAMMPGGLPVGPPCAFDAIAGMQMPSPHCGGIKRRKNQARKVVCIPAPAASAGGRTTGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRTDPSLLVITYNSEHNHPWPTQRNALAGSTRSHHAKSSKNKYSSHSLPQTPNLKAEPEHHQASGAVPSATAATAATATSTTTTTSTTSNSTPPPPPTMAVKEEAIMVGSEMEKVIDHHDASGWLDHGDLMQQMFSQSYYRPMIPEAAGGGLHADDFFADLAELESDPMSLIFPAGGEPGKENKSLDADPLFNMLDWGATNVAATSSFEQDESGW
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTGCGACTACTTCCTACAAAGGATGGAGGACGACCGCCACCACCACCATCAGGCCGGAGGAGACCTCACGGACGTCGTCCGAGCCGGCGGCGCGATGCATCCGGCAGCTGTCGCGGAGCTCTCCTCCACGGCCACCACGGGGTGGCAGCTCCCGGCCGAGCCAGCTGGACCACCCGGCCTCTTCCCGCCAGCCCGGTCATCGTCGTCGTCGGACGGCGGCGCCTTCGCCGGCCTCTCGGGCCCATTCGCCAGCGACTTCCGCGCGGACTCCGCCGGCGTTGACCCCAGCCCTGCCGACTTCTTTGACTTCGAGGCGCCCGCTGTCGGTGGCGGCCGGGGCGGAATGGTGGAGGGCGGCGGGCCGCCGCAGATGCCCGCGCTGTCGCCGAGTGAGGTACGGCCGTACTCGGTGATGATGGGCGGCGACACCGTCAAGATCGGCGTGCCGGCGATGATGCCCGGGGGGCTGCCCGTTGGCCCGCCGTGCGCGTTCGACGCCATCGCCGGGATGCAGATGCCGTCGCCGCACTGCGGCGGGATCAAGCGCAGGAAAAACCAGGCAAGGAAGGTGGTCTGCATCCCAGCACCTGCAGCATCAGCAGGAGGAAGGACCACTGGGGAGGTTGTTCCTTCTGATCTCTGGGCTTGGAGGAAGTATGGACAGAAGCCTATCAAAGGATCACCGTATCCAAGAGGGTACTACAGATGCAGCAGCTCTAAAGGATGCCCGGCGCGGAAGCAGGTGGAGCGCAGCCGGACAGACCCAAGCCTGCTCGTCATCACCTACAACTCGGAGCACAACCACCCTTGGCCGACGCAGCGCAACGCGCTCGCCGGCTCAACACGTTCCCACCACGCCAAGAGCAGCAAGAACAAGTACTCCTCGCACAGCCTGCCGCAGACGCCAAACCTCAAAGCGGAACCTGAGCATCACCAGGCCTCGGGGGCAGTCCCCAGCGCCACCGCCGCAACCGCAGCAACAGCCACAAGCACCACAACGACCACGAGCACCACCAGCAACAGCACCCCTCCTCCTCCTCCGACGATGGCTGTGAAAGAGGAGGCAATAATGGTGGGGTCGGAAATGGAGAAAGTGATAGACCACCATGACGCTTCTGGCTGGCTGGATCACGGTGATCTCATGCAGCAGATGTTCAGCCAGAGCTACTACAGGCCGATGATACCGGAGGCGGCCGGCGGTGGCCTCCATGCCGATGACTTCTTCGCTGACCTCGCCGAGCTGGAGTCGGATCCCATGAGCCTCATCTTCCCTGCAGGTGGTGAACCTGGAAAGGAGAACAAGAGCTTGGATGCCGATCCATTATTCAACATGCTAGACTGGGGTGCCACTAATGTTGCAGCTACTTCTTCATTTGAGCAAGACGAGAGTGGCTGG
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