PCR Condition:
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5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr |
Nucleotide Sequence:
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ATGAAGAACATGACCAGACAGGAGTACGTCGCTCACCTGAGAAGGAAAAGCAGCGGCTTCTCGCGCGGCGCTTCGATCTACCGGGGAGTCACCAGGCATCACCAGCACGGGCGGTGGCAGGCGCGCATCGGCCGCGTCTCGGGCAACAAGGACCTCTACCTCGGAACGTTCAGCACCCAGGAGGAGGCGGCGGAGGCGTACGACGTGGCCGCGATCAAGTTCCGCGGCCTCAGCGCGGTCACCAACTTCGACATCACGCGGTACGACGTGGACAAGATCATGGAGAGCAGCACGCTGCTCCCGGGCGAGCAGGTCCGGCGCAGGAAGGAAGGCGCCGACGCCGCGGTCTCGGAGGCCGCCGCCGCGCTGGTGCAGGCCGGCAACTGCATGACGGACACCTGGAAGATCAAGGCGGCGCTGCCGGCTGCCGCGCGGGCCGACGAGCGCGGCGCCGGCCAGCAGCAGCGTCAGGACTTGCTGTCGAGCGAGGCCTTCTCGCTGCTCCACGACATCGTGTCCGTCGACGCCGCTGCTGGTACAGGGACAGGGGGCATGTCGAACGCGTCCTCGTCGCTGGCCCCCAGCGTGAGCAACTCCCGGGAGCAGAGCCCGGACCGGGGCGGCGCCAGCCTCGCCATGCTCTTCGCCAAGCCCGCCGCGGCGCCCAAGCTGGCTTGCCCGCTGCCGCTGGGGTCCTGGGTGTCGCCGTCCGCGGTGTCCGCCAGGCCGCCCGGCGTGTCAATCGCGCACCTGCCGGTGTTCGCCGCGTGGACCGACGCA |
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TFome clones do not contain stop codon at 3'end.
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translation:
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MKNMTRQEYVAHLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVSGNKDLYLGTFSTQEEAAEAYDVAAIKFRGLSAVTNFDITRYDVDKIMESSTLLPGEQVRRRKEGADAAVSEAAAALVQAGNCMTDTWKIKAALPAAARADERGAGQQQRQDLLSSEAFSLLHDIVSVDAAAGTGTGGMSNASSSLAPSVSNSREQSPDRGGASLAMLFAKPAAAPKLACPLPLGSWVSPSAVSARPPGVSIAHLPVFAAWTDA
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