| TFome pUT5752 | |
|---|---|
| Template | BT037983.1 |
| Genbank Insert ID | KJ727800 |
| Protein Name | ZmEREB184 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM2G028151 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C409A p.Q137K Not present in functional domain. Not present in template FlcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5752
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B388_F |
| Temperature (ºC) | 66.4 |
| Sequence
|
caccATGAAGAACATGACCAGACAGGAGTAC |
3' Primer
| Name | B388_R |
| Temperature (ºC) | |
| Sequence
|
TGCGTCGGTCCACGCGG |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MKNMTRQEYVAHLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVSGNKDLYLGTFSTQEEAAEAYDVAAIKFRGLSAVTNFDITRYDVDKIMESSTLLPGEQVRRRKEGADAAVSEAAAALVQAGNCMTDTWKIKAALPAAARADERGAGQQQRQDLLSSEAFSLLHDIVSVDAAAGTGTGGMSNASSSLAPSVSNSREQSPDRGGASLAMLFAKPAAAPKLACPLPLGSWVSPSAVSARPPGVSIAHLPVFAAWTDA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAAGAACATGACCAGACAGGAGTACGTCGCTCACCTGAGAAGGAAAAGCAGCGGCTTCTCGCGCGGCGCTTCGATCTACCGGGGAGTCACCAGGCATCACCAGCACGGGCGGTGGCAGGCGCGCATCGGCCGCGTCTCGGGCAACAAGGACCTCTACCTCGGAACGTTCAGCACCCAGGAGGAGGCGGCGGAGGCGTACGACGTGGCCGCGATCAAGTTCCGCGGCCTCAGCGCGGTCACCAACTTCGACATCACGCGGTACGACGTGGACAAGATCATGGAGAGCAGCACGCTGCTCCCGGGCGAGCAGGTCCGGCGCAGGAAGGAAGGCGCCGACGCCGCGGTCTCGGAGGCCGCCGCCGCGCTGGTGCAGGCCGGCAACTGCATGACGGACACCTGGAAGATCAAGGCGGCGCTGCCGGCTGCCGCGCGGGCCGACGAGCGCGGCGCCGGCCAGCAGCAGCGTCAGGACTTGCTGTCGAGCGAGGCCTTCTCGCTGCTCCACGACATCGTGTCCGTCGACGCCGCTGCTGGTACAGGGACAGGGGGCATGTCGAACGCGTCCTCGTCGCTGGCCCCCAGCGTGAGCAACTCCCGGGAGCAGAGCCCGGACCGGGGCGGCGCCAGCCTCGCCATGCTCTTCGCCAAGCCCGCCGCGGCGCCCAAGCTGGCTTGCCCGCTGCCGCTGGGGTCCTGGGTGTCGCCGTCCGCGGTGTCCGCCAGGCCGCCCGGCGTGTCAATCGCGCACCTGCCGGTGTTCGCCGCGTGGACCGACGCA
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