| TFome pUT5754 | |
|---|---|
| Template | BT036116.1 |
| Genbank Insert ID | KJ727802 |
| Protein Name | ZmALF18 |
| Species | Zea mays |
| TF Family | Alfin-like |
| Gene id | GRMZM2G008259 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5754
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B405_F |
| Temperature (ºC) | 68.4 |
| Sequence
|
caccATGGATATGGCCCCCGC |
3' Primer
| Name | B405_R |
| Temperature (ºC) | |
| Sequence
|
CTGCCTTATTTTCTTCGAGCTGCA |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MDGGGTHRTPEDVFRDFRARRAGMIKALTTDVEKFYQQCDPEKENLCLYGLPNETWEVNLPAEEVPPELPEPALGINFARDGMDEKDWLSLVAVHSDAWLLAVAFYFGARFGFDKESRKRLFVMINNLPTIYEVVTGTAKKQTKEKTPKSSSKSNKAGPKPPRQPEPNSRGSKMPPPKDEDDSGGEEEEEEEDHENTLCGSCGDNYGQDEFWICCDACETWFHGKCVKITPAKAEHIKHYKCPNCSGSGKRARA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACGGCGGCGGCACGCACCGCACGCCGGAAGACGTGTTCCGGGATTTCCGCGCGCGGCGGGCTGGTATGATCAAAGCGCTCACCACCGACGTGGAGAAGTTCTACCAGCAGTGTGACCCAGAGAAAGAGAATCTGTGTTTGTATGGTCTTCCCAATGAAACATGGGAAGTGAACTTGCCTGCAGAGGAGGTTCCTCCTGAACTTCCAGAGCCAGCTCTGGGAATTAATTTTGCTCGTGACGGGATGGATGAAAAAGATTGGCTGTCACTAGTCGCGGTGCACAGTGATGCTTGGTTGCTGGCTGTGGCCTTTTACTTCGGTGCAAGATTTGGTTTTGACAAAGAATCCAGGAAGCGCCTTTTTGTCATGATTAATAACCTCCCTACAATATATGAAGTTGTCACGGGAACTGCCAAGAAGCAAACCAAGGAGAAAACTCCGAAAAGCAGCAGCAAGAGCAATAAAGCTGGCCCAAAACCGCCACGCCAGCCAGAACCCAACTCAAGGGGTTCGAAGATGCCACCTCCGAAGGATGAGGATGACAGCGGAGGCGAGGAAGAGGAGGAAGAGGAAGATCACGAAAACACGCTGTGTGGTTCTTGTGGTGACAACTACGGACAGGATGAGTTCTGGATATGCTGCGATGCGTGCGAGACTTGGTTCCATGGCAAGTGTGTCAAGATCACTCCTGCCAAGGCCGAACACATCAAGCACTACAAGTGTCCGAACTGCAGCGGTAGTGGCAAGAGAGCCCGAGCA
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