TFome clone pUT5759

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B801_F
Temperature (ºC)	62.1
Sequence Copy to clipboard	caccATGAGGTGGGGACTTAGAAGCA

3' Primer

Name	B801_R
Temperature (ºC)
Sequence Copy to clipboard	GCCGCTCAGGTGCGCAGT

PCR Condition

5 l Phusion GC buffer 1 l primer forward 20mM 1 l

Amino Acid Sequence

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MRWGLRSSCKQRHKESLQPLEQESKGKSKASFSSFLPLAWLAKLTARNGVPATKVVHATSSAAKNTAQAATAFPSCFHKPASPSTASASRSSLSLASSSSSAADAEAAGIVPCRRDDATGGDTVAGIAPRRRSVGNDDARTEAATATARQLYHSRRHYSVGGDRDLPHLGHLVSLSPNPKAAPPTPAPVRTLTPTLPPLPSDSDTDEEKRTTARNRRRRRRRAVSGRRSFSSAKATVRVRSPRPPAAAVSELERFAVVRRTRDPHRAFRASMVEMIASKRMVGRPDELETLLACYLSLNADEHHDCIVKVFRQVWFELNNASRATATAPPQRTAHLSG

TFome clones do not contain stop codon at 3'end.

CDS

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ATGAGGTGGGGACTTAGAAGCAGCTGTAAGCAACGGCACAAGGAATCCCTGCAACCACTGGAGCAAGAGAGCAAGGGGAAGAGCAAGGCCAGCTTCTCGTCCTTCTTGCCCCTGGCATGGCTTGCCAAGCTCACGGCCAGGAACGGCGTGCCCGCTACCAAGGTCGTGCATGCGACGTCGTCCGCGGCGAAGAACACGGCTCAGGCCGCCACCGCATTCCCGTCCTGCTTCCACAAGCCCGCCAGCCCCAGCACCGCGTCGGCGTCGCGAAGCAGCCTGAGCCTGGCCTCTTCGTCATCATCCGCCGCCGACGCAGAGGCAGCCGGCATTGTCCCTTGCCGTCGCGACGACGCTACCGGCGGGGACACGGTGGCAGGCATCGCCCCGCGCCGCCGCTCGGTGGGCAACGATGACGCCAGGACCGAGGCCGCGACCGCGACCGCGCGGCAGCTGTACCACAGTCGTCGCCACTACTCGGTCGGTGGCGACCGCGACCTTCCTCACCTCGGCCACCTCGTCTCCCTCTCCCCGAACCCGAAGGCGGCGCCGCCGACACCGGCGCCGGTGCGGACGCTGACGCCGACGCTGCCCCCACTGCCTTCCGATTCCGACACGGACGAGGAGAAGCGGACGACGGCACGGAACCGCCGGCGCCGGCGCCGGCGCGCCGTCAGCGGGCGGCGGTCGTTCTCCTCGGCGAAGGCCACGGTGCGTGTGCGCTCGCCTAGGCCGCCTGCCGCGGCGGTGTCGGAGCTGGAGCGGTTCGCGGTGGTGCGGCGGACGCGGGACCCGCATCGCGCGTTCCGGGCGTCCATGGTGGAGATGATCGCGAGCAAGCGCATGGTGGGGCGTCCCGACGAGCTGGAGACGCTGCTGGCGTGCTACCTGTCGCTCAACGCCGACGAGCACCACGACTGCATCGTCAAGGTCTTCCGGCAGGTCTGGTTCGAGCTCAACAACGCCTCCCGTGCCACTGCCACCGCCCCGCCGCAGCGCACTGCGCACCTGAGCGGC