| TFome pUT5767 | |
|---|---|
| Template | BT036761 |
| Genbank Insert ID | KJ727815 |
| Protein Name | ZmOFP34 |
| Species | Zea mays |
| TF Family | OVATE |
| Gene id | GRMZM2G028982 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5767
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B839_F |
| Temperature (ºC) | 73.0 |
| Sequence
|
caccATGCAAACGCCCTTCGTGGACAAGAGCCCCCAGTCCC |
3' Primer
| Name | B839_R |
| Temperature (ºC) | |
| Sequence
|
CTCCTCGTCCTCGCGGAAGACGGCCTCCCAGA |
PCR Condition
5 l Phusion GC buffer 1 l primer forward 20mM 1 l
Amino Acid Sequence
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MQTPFVDKSPQSHRRDSASGRLKRRLARILTRSSCATNATPAGSRTAFVSLAKTNNTSAAADSSHPEPEAPPSPYFCTPCSYERPRVRGDSDTRSRRRSRSASLVHISVDCTGGAGAGAASGRHPVHSDAPLLQHLSVPARDVVVTKQSKGRSRGKPVARSPSASRRHCSSSWSSSSWGRARRPSSCLPTPYSCSSSSLSSTGTDDELAPFSGSDEEGGDEAETMTLFSSDSTSELYHPNGGGRTSSRRRSHHHRSRRRRSHHQRRSHHHRRSHRNISRRAPRRALPRTGDPAPAPSDAHFLPLVSVDTEKHRERDSVKKLMTTGAASAGMAVVKRSSNPYLDFRSSMMEMVLGRRIGSVGNMEELLGSYLSLNSPRHHPAILAAFEDVWEAVFREDEE
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCAAACGCCCTTCGTGGACAAGAGCCCCCAGTCCCACCGCCGGGACAGCGCCAGCGGCCGGCTGAAGCGACGGCTCGCGCGGATCCTCACGCGTTCCTCCTGCGCCACCAACGCCACGCCCGCCGGTTCGCGCACGGCCTTCGTCAGCCTCGCCAAGACCAACAACACCAGCGCCGCCGCCGACTCCTCTCACCCGGAGCCGGAAGCGCCGCCTTCCCCGTACTTCTGCACGCCTTGCAGCTACGAGCGGCCCAGGGTCCGCGGCGACAGCGACACCCGCTCGCGCCGCCGCAGCCGCAGCGCCTCGCTCGTACACATCTCCGTGGACTGCACGGGCGGGGCCGGGGCCGGGGCCGCCTCCGGCCGCCACCCCGTCCACTCTGACGCGCCGCTTCTGCAGCACCTGTCGGTGCCGGCGAGGGACGTCGTCGTCACGAAGCAAAGCAAGGGCAGGAGCAGGGGCAAGCCGGTGGCGCGGTCTCCTTCGGCGTCGAGGCGACACTGCTCCTCGTCGTGGTCCTCCTCCTCCTGGGGCCGCGCGCGGCGGCCCAGCAGCTGCTTGCCCACGCCTTACAGCTGCTCGTCGTCGTCGTTGTCCTCCACCGGAACGGACGACGAGCTCGCGCCGTTCAGCGGCAGCGACGAGGAGGGCGGCGACGAGGCCGAGACGATGACGCTCTTCTCGTCCGACTCCACGTCCGAGCTCTACCACCCCAACGGCGGCGGCAGAACCAGCAGCAGGAGGAGGAGTCATCATCACAGGAGCAGGAGGAGGAGGAGTCATCATCAAAGGAGGAGTCATCATCACAGGAGGAGTCACAGGAACATAAGTCGCCGCGCGCCACGGCGTGCGCTGCCGAGAACCGGCGACCCGGCGCCGGCACCATCCGACGCTCATTTCCTGCCTCTGGTGTCCGTGGACACGGAGAAGCACAGGGAACGCGACAGCGTCAAGAAGCTGATGACGACGGGCGCGGCGTCGGCGGGCATGGCGGTGGTGAAGCGCTCGAGCAACCCCTACCTGGACTTCCGCAGCTCGATGATGGAGATGGTGCTGGGGCGGCGCATCGGCAGCGTGGGGAACATGGAGGAGCTCCTGGGGTCGTACCTCTCGCTCAACTCGCCGCGCCACCACCCGGCCATCCTTGCCGCCTTCGAGGACGTCTGGGAGGCCGTCTTCCGCGAGGACGAGGAG
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