| TFome pUT5779 | |
|---|---|
| Template | BT061636.1 |
| Genbank Insert ID | KJ727826 |
| Protein Name | ZmALF12 |
| Species | Zea mays |
| TF Family | Alfin-like |
| Gene id | GRMZM5G893976 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G31T p.V11F SNP not present in template FLcDNA, not in functional domain. |
| Request Information | Deposited to ABRC |
TFome clone pUT5779
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B409_F |
| Temperature (ºC) | 73.7 |
| Sequence
|
caccATGGCGGACGCCGCCTCT |
3' Primer
| Name | B409_R |
| Temperature (ºC) | |
| Sequence
|
CTGCCTCGCCCTCTTGGAGCTGCAG |
PCR Condition
PCR Conditions: Volumes per reaction (25l): 5 l GC
Amino Acid Sequence
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MADAASGRGHFVYPPRSAEDIFKDYRARRSAILRALTHEVEDFYAQCDPDKENLCLYGYANEAWEVALPAEEVPTELPEPALGINFARDGMNRRDWLALVAVHSDSWLLSVAFYYAARLNRNDRKRLFGMINELQTVFEVVSGARQQQSKERSSMDNGGRAKPVKIENNGKVTDEAYEDDSDHGETLCGTCGGIYNADEFWIGCDMCERWYHGKCVKITPAKAESIKHYKCPSCSSKRARQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGGACGCCGCCTCTGGCCGCGGCCACTTCGTCTACCCTCCCCGCTCCGCCGAGGACATCTTCAAGGACTACCGCGCCCGCCGGTCCGCCATCCTCCGCGCCCTCACTCACGAGGTCGAGGACTTCTACGCGCAGTGCGATCCAGACAAGGAGAACCTGTGCCTGTACGGCTACGCGAACGAGGCGTGGGAGGTGGCGCTGCCGGCAGAGGAGGTGCCCACGGAGCTGCCGGAGCCTGCGCTGGGAATCAACTTCGCGCGCGACGGAATGAACCGCCGGGACTGGCTTGCGCTCGTCGCCGTCCACTCCGACTCGTGGCTCCTCTCCGTCGCCTTTTACTATGCCGCTCGGCTTAATCGTAATGACCGGAAGCGCTTATTTGGTATGATTAATGAATTGCAAACTGTTTTTGAAGTTGTCTCCGGTGCGAGACAACAACAATCTAAAGAGAGGTCCAGTATGGACAATGGTGGTAGAGCCAAGCCTGTAAAGATAGAAAATAATGGAAAAGTCACAGACGAAGCATACGAAGATGACAGTGATCACGGTGAAACCCTATGTGGAACCTGTGGTGGGATATACAACGCGGATGAGTTTTGGATCGGGTGTGACATGTGCGAGCGATGGTACCATGGCAAGTGTGTCAAGATAACTCCTGCCAAGGCAGAGAGCATAAAGCATTACAAATGCCCGAGCTGCAGCTCCAAGAGGGCGAGGCAG
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