| TFome pUT5787 | |
|---|---|
| Template | BT061291.1 |
| Genbank Insert ID | KJ727834 |
| Protein Name | ZmMADS42 |
| Species | Zea mays |
| TF Family | MADS |
| Gene id | GRMZM2G375707 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5787
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B637_F |
| Temperature (ºC) | 68.9 |
| Sequence
|
caccATGATGGCGAGGAGGGACA |
3' Primer
| Name | B637_R |
| Temperature (ºC) | |
| Sequence
|
CCACCATCCAGATCCAGAGCTTTG |
PCR Condition
Initial PCR reaction mix Volumes per reaction (25l
Amino Acid Sequence
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MMARRDRRSGVRFIEDGRDRSLTFFKRRSGLFKAASDLSTLTGARVAVVLESESGRFSSFGTPEASPIVDAFLLGSAPTDLDTSEADKASITNLQNEVFQLEKDKAMEDKRRKESLARSSRKVQQASKVARYVYGSVDDLDATELFEMYRELSRVQQEISDRLPTTLLHDASKADAGGGRGRLRDPSLLRPTWWRASAMPLPPLQQAATPPTKCPPWAPSQARFQFQQHPWAASSTSPPAVLPRSSGSPLPSSAILPSPLAPQNPSFLRHHHPLAPHTTPPTSAGVQFQAPRTPAPTEAQNPYTYYIRGIDIISGNSSHPSSLSPVMPSSSPTPHQQPSSLRAPPSDDSSPLSLSPRVSYPLHVGSPYHQQLPLSAQNHNSVQQPPPPPESCAHVVGSPLIVHSHQVFYSNLSPDLNAELGSVDDGGAGATHADGGLGLSSPQQSDGFDGVTPNKSFSAGQSSGSGWW
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGGCGAGGAGGGACAGGAGGTCAGGGGTGAGATTCATCGAGGACGGTAGGGACCGGAGCCTCACGTTCTTCAAGAGGCGGTCCGGGCTCTTCAAGGCGGCGTCCGACCTGTCCACCCTCACCGGCGCGAGGGTGGCGGTGGTGCTGGAGTCCGAGAGCGGGAGGTTCTCCTCCTTCGGCACCCCGGAGGCCAGCCCCATAGTGGACGCTTTCCTTCTCGGGAGCGCGCCGACGGACCTTGACACCAGCGAAGCAGACAAGGCTTCCATCACCAACCTGCAGAACGAGGTGTTCCAGCTGGAGAAAGACAAGGCCATGGAGGACAAGCGGAGGAAGGAGAGCCTGGCGCGGTCCAGCAGGAAGGTCCAGCAGGCCTCGAAGGTGGCCAGGTATGTTTACGGCAGCGTAGACGATCTTGACGCCACCGAGCTCTTCGAGATGTACCGTGAGCTATCGCGGGTGCAGCAAGAGATCAGCGATCGCTTGCCGACGACCTTGCTCCACGACGCGAGCAAAGCAGACGCCGGTGGTGGCCGTGGCCGTCTTAGAGACCCGTCGCTGCTGCGGCCCACTTGGTGGCGTGCAAGTGCAATGCCATTGCCGCCCCTGCAACAAGCTGCGACGCCACCGACGAAGTGTCCTCCATGGGCTCCCTCGCAGGCTCGCTTCCAGTTCCAGCAACATCCATGGGCGGCGTCATCCACGTCTCCTCCCGCTGTGCTGCCAAGGTCATCTGGCTCCCCGCTTCCGAGCTCGGCGATCCTCCCGTCACCGCTGGCGCCGCAGAATCCGTCCTTCTTGCGACATCATCACCCTCTGGCACCGCATACCACCCCTCCCACATCGGCCGGCGTGCAATTCCAAGCACCCAGGACGCCGGCACCTACGGAAGCCCAGAACCCTTACACCTACTACATCCGTGGGATCGACATCATCAGTGGTAACAGCTCGCACCCGTCGTCACTATCGCCTGTCATGCCGTCGTCTTCACCGACGCCCCACCAGCAGCCGTCGTCGTTGCGAGCTCCACCGTCAGATGATTCGTCTCCTCTATCTTTGTCGCCGCGAGTTTCATACCCGCTGCACGTAGGGTCGCCGTACCACCAACAGCTTCCTTTGAGTGCGCAGAATCACAACTCCGTGCAGCAGCCGCCGCCGCCGCCTGAGAGCTGTGCACATGTTGTTGGCTCACCTCTGATCGTCCATTCTCACCAAGTGTTCTACAGCAACTTGTCACCTGACCTAAATGCTGAGCTGGGAAGCGTCGATGATGGTGGAGCTGGAGCCACCCATGCTGACGGTGGACTTGGTTTGTCTAGCCCTCAGCAAAGTGATGGCTTCGACGGGGTGACGCCCAACAAGTCCTTCTCTGCAGGACAAAGCTCTGGATCTGGATGGTGG
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