| TFome pUT5953 | |
|---|---|
| Template | BT054888.1 |
| Genbank Insert ID | KJ727861 |
| Protein Name | ZmWRKY1 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G398506 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5953
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C174_F |
| Temperature (ºC) | 67.5 |
| Sequence
|
caccATGGCCGATTCGCCAAAC |
3' Primer
| Name | C174_R |
| Temperature (ºC) | |
| Sequence
|
TGGACCCATCACTAAGTTACCAGCACT |
PCR Condition
PCR Conditions: Volumes per reaction (50l): 10 l H
Amino Acid Sequence
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MADSPNPSSGDLPAGAGCSPEKPVLADRRVSALAGAGARYKAMSPARLPISREPCLTIPTGFSPGALLESPVLLNNFKVEPSPTTGTLSMAANMNKSTHPDILPSPRDNSADSGQEDGGSREFEFKPHLNSQSSATAVNNQNHHDIPMQNHGSNHASPSSNLMTENKPLCSRESSHTANVSSAPNQPVSIVCPSDNMPAEVGTSEMHLINSSENAAQETQTENVAEKSAEDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERSLDGQITEVVYKGHHNHPKPQPNRRLAAGAVPSSQAEERYDGVAPIEDKPSNIYSNLCNQAHSAGMVDNVPGPASDDDVDAGGGRPYPGDDSNDDDDLDSKRRKMESAGIDAALMGKPNREPRVVVQTVSEVDILDDGYRWRKYGQKVVKGNPNPRSYYKCTHTGCPVRKHVERASHDPKSVITTYEGKHNHEVPASRNASHEMSAAPMKPVVHPINSSMPGFGGMMRACDARAFNNQYSQAAESDTISLDLGVGISPNHSDATNQMQPSVPEPMQYQMRHMAPVYGSMGLPGMPVPAIPGNVVASSIYGSREEKGNEGFTFKAAPLDRSANLCYSSAGNLVMGP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCGATTCGCCAAACCCTAGCTCCGGCGACCTTCCCGCCGGCGCTGGGTGCTCGCCGGAGAAGCCGGTCCTGGCGGATCGACGTGTGTCTGCGCTCGCCGGCGCCGGCGCGAGGTACAAGGCCATGTCCCCCGCGCGGCTGCCGATATCGCGGGAGCCGTGCCTCACCATCCCCACCGGGTTTAGCCCCGGAGCTCTCCTCGAGTCCCCCGTCCTCCTCAACAACTTCAAGGTTGAACCGTCTCCCACAACCGGTACTCTGAGCATGGCTGCAAACATGAACAAGAGCACTCATCCGGACATACTGCCTTCGCCTAGGGATAATTCAGCTGATAGTGGCCAAGAAGATGGAGGCTCTCGGGAGTTTGAATTCAAGCCTCATCTCAATTCTCAATCGTCGGCTACTGCAGTCAACAATCAAAATCATCATGACATTCCTATGCAAAATCATGGCTCAAATCATGCCTCACCATCTAGCAATTTGATGACTGAAAATAAACCTCTATGCTCACGGGAATCGAGTCACACAGCAAATGTTTCAAGTGCTCCAAACCAACCTGTTTCAATAGTTTGTCCATCTGACAACATGCCTGCTGAAGTTGGTACATCAGAGATGCACCTGATAAATAGCTCTGAAAATGCTGCCCAGGAGACACAAACTGAAAATGTAGCTGAAAAATCAGCAGAGGATGGCTATAATTGGCGCAAATATGGACAGAAGCATGTCAAGGGAAGTGAAAATCCTAGAAGTTATTACAAGTGCACTCATCCTAATTGTGAAGTTAAAAAGCTATTAGAGCGTTCGCTTGATGGTCAGATTACTGAAGTTGTTTATAAAGGGCATCATAATCATCCCAAGCCCCAACCAAATAGAAGGTTAGCTGCTGGTGCAGTTCCTTCAAGCCAGGCTGAAGAAAGATACGATGGTGTGGCACCTATTGAAGACAAACCTTCAAATATTTATTCCAACCTCTGTAACCAAGCACATTCAGCTGGCATGGTTGATAATGTTCCGGGTCCAGCAAGTGATGATGATGTTGATGCTGGAGGTGGAAGACCCTACCCTGGGGATGACTCTAATGATGATGATGACTTGGACTCAAAACGCAGGAAAATGGAATCTGCTGGTATCGATGCTGCTTTGATGGGTAAACCAAATCGCGAGCCCCGTGTCGTTGTACAAACTGTTAGTGAAGTTGATATCTTGGATGATGGGTATCGCTGGCGCAAATATGGGCAGAAAGTAGTGAAAGGAAACCCTAACCCACGGAGTTACTACAAATGCACACATACAGGATGCCCAGTCAGGAAACATGTTGAGAGAGCATCACATGACCCGAAGTCAGTGATCACAACATATGAAGGAAAACATAACCATGAAGTCCCTGCTTCCAGGAATGCAAGCCATGAGATGTCTGCAGCTCCCATGAAGCCGGTGGTGCATCCTATTAACAGCAGCATGCCAGGCTTTGGTGGCATGATGAGAGCATGCGATGCCAGGGCCTTCAACAATCAATATTCTCAGGCAGCCGAAAGTGACACCATCAGTCTTGACCTTGGTGTAGGTATCAGCCCTAACCACAGCGATGCAACAAACCAGATGCAGCCCTCAGTTCCGGAACCTATGCAGTATCAGATGCGACACATGGCTCCTGTGTACGGTAGCATGGGACTTCCAGGAATGCCTGTGCCAGCAATACCTGGAAATGTAGTAGCTAGCAGCATATACGGCTCCAGAGAAGAAAAAGGAAACGAAGGGTTTACTTTCAAAGCTGCACCTTTGGACCGATCAGCTAACTTATGTTACAGTAGTGCTGGTAACTTAGTGATGGGTCCA
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