| TFome pUT5954 | |
|---|---|
| Template | BT054518.1 |
| Genbank Insert ID | KJ727862 |
| Protein Name | ZmPHD13 |
| Species | Zea mays |
| TF Family | PHD |
| Gene id | GRMZM2G059266 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5954
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C360_F |
| Temperature (ºC) | 70.5 |
| Sequence
|
caccATGGCCTCCGACGGTGC |
3' Primer
| Name | C360_R |
| Temperature (ºC) | |
| Sequence
|
TTTTCCTGGAGCAGCAGCTGCTG |
PCR Condition
PCR Conditions: Volumes per reaction (50l): 10 l H
Amino Acid Sequence
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MASDGAPRGRRPKRPASGGLVQPDVLTYKRRRRGTGANAGHSAVTSGPDPDPVKNQMSAVVHATNSQQGGRQTLDRHWRSWRDTLEGVLRSTAVNQSAGGIQNCIRDALRYNGWHPTEHGNLVDDRGTDEEDATGALHSEESSGALVKLEDGTVASLEANKAMCHDTLFDILISEKFAMLCDLLAATFHGSKPDDVIGLQIIDAKMRNGDYAQNPALFDHDIKQIWKKFEHVGQEMAGLASSLLLISQASNQKQASDISEIDVAEHKIEETSLVGVTRKALRKFTPPCDSGYSTIPRRTGTSGSDGICKDCGRKADSKGRIICDRCEAAYHVSCLKLAIDEEAPAKWYCPTCVEPDVPLKSNNHGRAHEGCDVCEWLEVEKPEEPAEEPELAVKTQESSVSSIVEDSEPDLSITALANLCKLCGTCEDENKKFVVCGHGYCSFKFYHALCLKESQIASEKQRNLKCWYCPSCLCRRCFKNKDDEKIVLCDGCDEAYHTYCMDPPRSSVPRGKWFCTPCSAWRSANGMQRYEKSILQSVKRVPDAKGPKVQAAAAAPGK
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCTCCGACGGTGCCCCCAGAGGCCGCCGTCCCAAGCGCCCCGCTTCGGGAGGCCTGGTCCAACCCGACGTCCTCACCTACAAGAGACGACGCCGCGGCACCGGCGCCAACGCCGGCCACAGCGCCGTCACGTCGGGCCCCGACCCCGATCCCGTCAAGAATCAGATGAGCGCGGTGGTGCACGCCACCAATTCCCAGCAAGGTGGGCGCCAAACGCTGGACAGGCACTGGAGAAGCTGGAGGGACACGCTGGAGGGCGTCCTTCGATCCACTGCTGTGAACCAGAGCGCCGGAGGGATTCAGAACTGCATCCGAGATGCGCTCAGATATAATGGTTGGCATCCCACAGAACATGGAAACTTAGTTGATGACCGAGGAACAGACGAAGAGGACGCTACTGGAGCTCTACATTCTGAAGAGAGTAGTGGTGCCTTGGTTAAGTTAGAAGATGGAACAGTTGCATCTTTGGAAGCCAACAAGGCGATGTGCCACGACACTCTCTTTGACATTCTAATCTCCGAGAAGTTTGCCATGTTGTGTGATCTGCTAGCTGCAACATTCCACGGCAGTAAGCCAGACGACGTTATTGGTTTGCAAATAATTGATGCCAAGATGAGAAATGGGGATTATGCACAGAACCCTGCGCTGTTTGACCATGATATTAAGCAGATATGGAAGAAGTTTGAACACGTTGGCCAAGAAATGGCTGGTCTGGCAAGCAGTCTCTTACTGATTTCACAAGCTTCTAATCAAAAGCAGGCTTCTGACATTTCAGAAATTGATGTGGCTGAACACAAGATAGAGGAAACAAGTCTGGTTGGTGTCACCCGCAAAGCCCTAAGGAAGTTCACTCCCCCATGCGATTCTGGCTATTCTACCATACCAAGACGAACTGGGACATCTGGATCGGATGGAATTTGCAAGGATTGTGGTAGAAAGGCAGACAGCAAAGGAAGAATTATCTGTGACAGGTGTGAAGCCGCGTACCATGTTTCATGTCTCAAGCTTGCCATTGATGAAGAGGCCCCAGCAAAATGGTACTGTCCCACCTGCGTCGAACCAGACGTACCTTTAAAGAGCAACAACCATGGCAGAGCACATGAAGGCTGCGATGTATGCGAGTGGCTTGAGGTGGAGAAGCCAGAAGAACCTGCCGAAGAACCTGAATTGGCCGTCAAAACACAGGAGAGCTCAGTGTCAAGCATTGTCGAAGACAGTGAACCAGACCTCTCGATAACCGCTCTAGCAAACTTGTGCAAGCTTTGTGGCACGTGTGAAGATGAGAACAAGAAGTTCGTGGTATGCGGGCATGGCTACTGTTCTTTCAAGTTCTATCATGCCCTGTGCCTGAAAGAAAGCCAGATTGCAAGCGAGAAGCAGAGGAATCTAAAATGCTGGTACTGCCCGTCTTGCCTGTGCAGACGCTGCTTCAAGAACAAGGACGACGAGAAAATAGTGTTGTGTGATGGCTGTGACGAAGCTTACCACACTTACTGCATGGATCCGCCGCGCAGTTCTGTCCCGAGAGGTAAATGGTTCTGCACGCCGTGTAGTGCATGGAGGTCGGCGAACGGGATGCAGAGGTATGAGAAGTCGATCCTGCAGAGCGTCAAGCGTGTTCCTGATGCCAAAGGGCCCAAGGTGCAGGCAGCAGCTGCTGCTCCAGGAAAA
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