| TFome pUT5957 | |
|---|---|
| Template | BT041066.1 |
| Genbank Insert ID | KJ727865 |
| Protein Name | ZmMYBR88 |
| Species | Zea mays |
| TF Family | MYB-related |
| Gene id | GRMZM2G064328 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5957
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C165_F |
| Temperature (ºC) | 70.6 |
| Sequence
|
caccATGGCCACAACGGCGACA |
3' Primer
| Name | C165_R |
| Temperature (ºC) | |
| Sequence
|
ACTCATGCTAGCCACGGGCTTTG |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MATTATTMAPVTAPVATANLEPTPTPLHPRPAAPPHLRALSTLAPVKSEAPPTPSSSTAITAAAGAEDPSYIITVPSYSAWFSFDSIHDTERRLLPEFFVGEAAAASGCRGPHAYKYYRDSLIRRFRARPGRRLTLTEARRGLVGDVGSVRRVFDFLEEWGLINYGALPSGSKQAKEKREEAAQQSSLPFGAIAPRKLCTGCRTVCGLAYYACDKADISLCARCYVNNNYRPGLSPANFKRVEITEDAKPDWTDKETLHLLEAVLHYGEDWKKVSEHVSSRSEKDCIARFIRLPFGEQFMGHKEDRMRFENTDDNTDEPGANVSKRLRLTPLADASNPIMAQVAFLSAIVGSDVASAAAQAAISAQSRVDLNDGEIETSINSIKQEESSHTNGLSANVLLKEAAANARAQLEKERNSIEQSLSNIVDVQMKEIQDKICRFEQKEMLMEKERQQLHFLRDLLFTDQLAVMQHQQRSPAVATECKGDEKPKPVASMS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCACAACGGCGACAACGATGGCGCCGGTGACGGCGCCGGTGGCCACAGCCAACCTCGAACCTACACCAACACCTCTGCATCCACGTCCTGCCGCGCCACCTCATCTCCGGGCGCTTTCCACCTTGGCCCCCGTCAAGTCAGAGGCTCCTCCCACCCCCTCGTCTTCTACCGCCATCACCGCCGCAGCCGGAGCGGAGGATCCATCCTACATCATCACCGTCCCTAGTTATTCAGCGTGGTTCTCGTTCGACTCCATCCACGACACGGAGCGCCGCCTCCTGCCGGAGTTCTTCGTGGGGGAGGCCGCCGCGGCGTCAGGGTGCCGGGGCCCGCACGCGTACAAGTACTACCGCGACTCCCTGATACGGAGGTTCCGGGCGCGGCCGGGTCGACGGCTCACGCTCACGGAGGCTCGGAGGGGCCTTGTTGGCGACGTCGGCTCAGTCCGCCGCGTCTTCGATTTCCTTGAGGAATGGGGGCTTATCAACTACGGTGCTTTGCCATCGGGGTCAAAGCAAGCTAAAGAGAAGAGGGAAGAGGCTGCACAACAGTCCTCGTTGCCTTTTGGGGCAATTGCACCCAGGAAGCTCTGTACCGGTTGCCGCACCGTGTGTGGGCTTGCTTACTACGCCTGCGACAAGGCAGATATAAGTCTTTGTGCTAGATGCTATGTAAATAATAACTACCGGCCGGGCCTGTCTCCTGCTAACTTCAAGAGAGTTGAAATTACTGAAGATGCAAAACCAGATTGGACAGACAAAGAAACTCTTCACTTGCTTGAGGCTGTCTTGCATTATGGGGAAGACTGGAAAAAGGTGTCTGAACATGTTAGTAGTCGGTCAGAAAAAGATTGTATTGCAAGATTTATAAGGCTACCTTTTGGAGAACAGTTCATGGGACACAAGGAGGATAGAATGAGGTTTGAGAATACTGATGACAATACTGATGAGCCTGGAGCAAATGTCTCAAAACGACTACGTCTCACTCCACTAGCAGATGCAAGCAATCCGATTATGGCTCAGGTTGCATTTTTATCAGCCATTGTGGGTTCCGATGTTGCCTCAGCTGCAGCGCAAGCAGCTATTTCTGCCCAATCCCGTGTTGACCTTAATGACGGCGAGATTGAAACTTCAATAAATAGCATTAAGCAAGAAGAATCATCTCACACAAATGGGCTCTCAGCCAACGTGTTACTGAAAGAGGCAGCTGCTAATGCACGGGCACAACTCGAGAAGGAAAGGAACAGCATAGAGCAATCTTTATCAAACATAGTAGACGTCCAGATGAAGGAAATCCAAGATAAAATATGCCGGTTTGAGCAGAAGGAGATGCTGATGGAGAAAGAGCGGCAGCAGCTCCATTTTCTAAGGGATCTACTTTTCACAGACCAATTGGCAGTCATGCAGCATCAACAAAGGAGTCCAGCTGTAGCCACAGAGTGCAAGGGTGATGAGAAGCCAAAGCCCGTGGCTAGCATGAGT
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