| TFome pUT5958 | |
|---|---|
| Template | BT036023.1 |
| Genbank Insert ID | KJ727866 |
| Protein Name | ZmbZIP1 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM2G015534 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G421A p.E141K; c.A1002T p.S334(=). SNPs present in template FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5958
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B473_F |
| Temperature (ºC) | 72.1 |
| Sequence
|
caccATGCCGCCGACGACCCA |
3' Primer
| Name | B473_R |
| Temperature (ºC) | |
| Sequence
|
ATACATGTCCATGTGTATGGCCCCATTTG |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MPPTTHHIYGYLLLGMEHVISMEEILGPFWELLPPPAPEPEPEREQPPVTGIVVGSVIDVAAAGHGHGGGDMMDQQHATEWTFERLLEEEALTTSTPPPVVVVPNSCCSGALNVDRPPVMEEAVMMAPAAVSSAVVGDPMKYNAILRRKLEEDLEAFKMWRAASSVVTSDQRSQGSNNHTGGSSIRNNPVQNKLMNGEDPINNNHAQTAGLGVRLATSSSSRDPSPSDEDMDGEVEILGFKMPTEERVRKRKESNRESARRSRYRKAAHLKELEDQVAQLKAENSCLLRRIAALNQKYNDANVDNRVLRADMETLRAKVKMGEDSLKRVIEMSSSVPSSMPISAPTPSSDAPVPPPPIRDSIVGYFSATAADDDASVGNGFLRLQAHQEPASMVVGGTLSATEMNRVAAATHCAGAMELIQTAMGSMPPTSASGSTPPPQDYELLGPNGAIHMDMY
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCCGCCGACGACCCATCATATCTATGGTTACTTATTATTGGGCATGGAGCACGTCATCTCAATGGAGGAGATCCTCGGGCCCTTCTGGGAGCTGCTACCACCGCCAGCGCCAGAGCCAGAGCCAGAGCGAGAGCAGCCTCCGGTAACCGGCATCGTCGTCGGCAGTGTCATAGACGTTGCTGCTGCTGGTCACGGTCATGGTGGCGGCGACATGATGGATCAGCAGCACGCCACAGAGTGGACCTTTGAGAGGTTACTGGAAGAGGAGGCTCTGACGACAAGCACACCGCCGCCGGTGGTGGTGGTGCCGAACTCTTGTTGCTCAGGCGCCCTAAATGTTGACCGGCCGCCGGTGATGGAAGAGGCGGTAATGATGGCGCCTGCGGCGGTGAGTAGTGCCGTAGTAGGGGACCCCATGAAGTACAATGCCATACTGAGGAGGAAGCTGGAGGAGGACCTCGAGGCCTTCAAAATGTGGAGGGCGGCCTCCAGTGTTGTGACCTCAGATCAACGTTCTCAAGGCTCAAACAATCACACTGGAGGTAGCAGCATCAGGAATAATCCAGTGCAGAACAAGCTGATGAACGGCGAAGATCCAATCAACAATAACCACGCTCAAACCGCAGGCCTTGGCGTGAGGCTTGCTACTAGCTCTTCCTCGAGAGATCCTTCACCATCAGACGAAGACATGGACGGAGAAGTAGAGATTCTGGGGTTCAAGATGCCTACCGAGGAAAGAGTGAGGAAAAGAAAGGAATCCAATAGAGAATCAGCCAGACGCTCGAGATACAGGAAAGCCGCTCACCTGAAAGAACTGGAGGACCAGGTAGCACAGCTAAAAGCCGAGAATTCTTGCCTGCTGAGGCGCATTGCCGCTCTGAACCAGAAGTACAACGACGCTAACGTCGACAACAGGGTGCTGAGAGCGGACATGGAGACCCTAAGAGCTAAGGTGAAGATGGGAGAGGACTCCCTGAAGCGGGTGATAGAGATGAGCTCTTCAGTGCCGTCGTCCATGCCCATCTCGGCGCCGACCCCCAGCTCCGACGCTCCAGTGCCGCCGCCGCCTATCCGAGACAGCATCGTCGGCTACTTCTCCGCCACAGCCGCAGACGACGATGCTTCGGTCGGCAACGGTTTCTTGCGACTGCAAGCTCATCAAGAGCCTGCATCCATGGTCGTCGGTGGAACTCTGAGCGCCACAGAGATGAACCGAGTAGCAGCAGCCACGCATTGCGCGGGGGCCATGGAGCTCATCCAGACGGCGATGGGATCCATGCCGCCGACCTCCGCCTCCGGATCTACACCGCCGCCGCAGGATTATGAGCTGCTGGGTCCAAATGGGGCCATACACATGGACATGTAT
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