| TFome pUT5960 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727868 |
| Protein Name | ZmEREB133 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM2G100727 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5960
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE0052_F |
| Temperature (ºC) | 70.0 |
| Sequence
|
caccATGCATGGCGCGCGCGGA |
3' Primer
| Name | SE0052_R |
| Temperature (ºC) | |
| Sequence
|
CTGAGGTGGACGAGTTGACGGTGGATACTGGC |
PCR Condition
5 l GC buffer 5 l 5x CES 1.5l MgCl2 50mM 1 l dNTPs
Amino Acid Sequence
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MHGARGSQRRGQKRDAADARYISVALQPNATSCARVVVLCCVPVWLCAHLDLHIVSTHAGGERAVHDDVQRAAAMEGHYSPTAAPEVSGERRFRGVRQRPWGKWAAEIRDPHKAARVWLGTFETAEAAARAYDEAALRFRGSRAKLNFPEDARLTTPSSAAATAATAAAGSTRTMAVASTGGYPASAASADYLQYQMFLQGPQGATSGSHGGGYPLYYDYGGHSGVGDGSVSSSSGSYSFPASTVTVASVPSSAPSYDEAAQWTSWPESSASAWSYPATTGSWSASSQYPPSTRPPQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCATGGCGCGCGCGGAAGCCAACGCAGGGGTCAAAAGCGCGACGCCGCCGACGCGCGCTATATATCCGTCGCATTGCAACCTAACGCTACGTCGTGTGCGCGAGTAGTAGTGCTGTGCTGTGTGCCTGTGTGGCTGTGTGCTCATCTCGATCTACATATCGTGTCCACGCACGCAGGAGGAGAGCGGGCGGTCCACGACGACGTGCAGCGCGCGGCGGCCATGGAGGGCCACTACTCGCCCACGGCCGCCCCCGAGGTGAGCGGCGAGCGGCGGTTCCGCGGCGTGCGGCAGCGGCCGTGGGGCAAGTGGGCGGCTGAGATCCGGGACCCGCACAAAGCGGCCCGCGTCTGGCTCGGCACGTTCGAGACCGCCGAGGCCGCGGCGCGTGCCTACGACGAGGCCGCGCTCCGCTTCCGCGGCAGCCGCGCCAAGCTTAACTTCCCCGAGGACGCGCGCCTCACCACGCCGTCGTCCGCCGCGGCCACGGCCGCCACCGCGGCGGCGGGATCCACGAGGACGATGGCCGTGGCCTCCACGGGAGGGTACCCGGCCAGCGCCGCCTCTGCTGACTACCTGCAGTACCAGATGTTTCTGCAGGGTCCGCAGGGAGCCACCAGCGGCAGTCATGGAGGGGGGTATCCTCTGTACTACGACTATGGCGGCCACAGCGGGGTTGGCGATGGTTCCGTGAGCAGTTCTTCGGGCTCCTACTCTTTTCCGGCCTCCACGGTTACCGTGGCTTCTGTGCCCTCCTCTGCTCCGAGCTACGACGAGGCAGCGCAGTGGACAAGCTGGCCGGAGAGTAGTGCTAGTGCGTGGAGCTATCCGGCGACCACGGGTTCTTGGTCTGCTTCAAGCCAGTATCCACCGTCAACTCGTCCACCTCAG
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