| TFome pUT5962 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727870 |
| Protein Name | ZmRAV1 |
| Species | Zea mays |
| TF Family | RAV |
| Gene id | GRMZM2G169654 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G193A p.A65T Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5962
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE0066_F |
| Temperature (ºC) | 63.6 |
| Sequence
|
caccATGGACAGCGCCAGCAG |
3' Primer
| Name | SE0066_R |
| Temperature (ºC) | |
| Sequence
|
CACTAGTGCCAGCTCCACGAGCT |
PCR Condition
5 l GC buffer 1.5l MgCl2 50mM 1.25 l DMSO 100% 1 l
Amino Acid Sequence
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MDSASSLVDDTSSGGGGGASTDKLRALAVFAAASGTPLERMGSGASAVVDAAEPGAEADSGSGATAVSVGGKLPSSRYKGVVPQPNGRWGAQIYERHQRVWLGTFAGEADAARAYDVAAQRFRGRDAVTNFRPLADADPDAAAELRFLASRSKAEVVDMLRKHTYFDELAQNKRAFAAASASAATASSLANNPSSYASLSPATATAAAREHLFDKTVTPSDVGKLNRLVIPKQHAEKHFPLQLPSAGGESKGVLLNLEDAAGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKGLQAGDVVGFYRSAAGADTKLFIDCKLRPNSVVVASTAGPSPPAPVAKAVRLFGVDLLTAPATAAAPAEAVAGCKRARDLGSPPQAAFKKQLVELALV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACAGCGCCAGCAGCCTCGTGGACGACACCAGTAGCGGTGGCGGCGGCGGCGCGTCCACGGACAAGCTAAGGGCTCTGGCCGTCTTCGCCGCCGCCTCGGGGACGCCGCTGGAGCGCATGGGCAGCGGCGCCAGCGCGGTCGTGGACGCGGCCGAGCCGGGCGCCGAGGCGGACTCCGGTTCCGGTGCCACCGCGGTGAGCGTCGGCGGGAAGCTGCCGTCGTCAAGGTACAAGGGCGTGGTGCCGCAGCCCAACGGGCGGTGGGGCGCGCAGATCTACGAGCGCCACCAGCGCGTGTGGCTCGGCACCTTCGCGGGCGAGGCCGACGCGGCGCGCGCCTACGACGTCGCGGCGCAGCGGTTCCGCGGCCGCGACGCGGTCACCAACTTCCGCCCGCTCGCGGACGCCGACCCGGACGCCGCCGCCGAGCTCCGGTTCCTGGCGTCCCGCTCCAAGGCCGAGGTCGTCGACATGCTCCGCAAGCACACCTACTTCGACGAGCTCGCGCAGAACAAGCGCGCCTTCGCCGCCGCGTCCGCGTCCGCGGCCACCGCCTCGTCGCTGGCCAACAACCCTTCTTCCTACGCGTCGCTCTCCCCCGCGACCGCGACGGCCGCCGCGCGGGAGCACCTCTTCGACAAGACGGTCACCCCCAGCGACGTGGGCAAGCTGAACCGGCTGGTGATCCCGAAGCAGCACGCCGAGAAGCACTTCCCGCTGCAGCTCCCATCCGCCGGCGGCGAGAGCAAGGGCGTGCTCCTCAACCTGGAGGACGCCGCGGGCAAGGTGTGGCGGTTCCGCTACTCGTACTGGAACAGCAGCCAGAGCTACGTGCTCACCAAGGGCTGGAGCCGCTTCGTCAAGGAGAAGGGCCTCCAAGCCGGCGACGTCGTCGGCTTCTACCGCTCCGCTGCCGGCGCCGACACCAAGCTCTTCATCGACTGCAAGCTGCGGCCCAACAGCGTCGTCGTCGCCTCGACGGCAGGCCCGTCGCCTCCGGCGCCGGTGGCGAAGGCCGTGCGTCTCTTCGGCGTCGACCTGCTGACGGCACCGGCCACCGCCGCGGCGCCGGCGGAGGCCGTGGCCGGGTGCAAGAGAGCCAGGGACTTGGGTTCGCCCCCGCAGGCGGCGTTCAAGAAGCAGCTCGTGGAGCTGGCACTAGTG
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