TFome clone pUT5963

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	SE0067_F
Temperature (ºC)	61.7
Sequence Copy to clipboard	caccATGGAACAGGGGGACGATC

3' Primer

Name	SE0067_R
Temperature (ºC)
Sequence Copy to clipboard	GCTAAAACAGCATGGACAGTGAGCA

PCR Condition

5 l GC buffer 5 l 5x CES 1.5l MgCl2 50mM 1 l dNTPs

Amino Acid Sequence

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MEQGDDHRRKMGSRPPWCACDRAGNTAAMKKGPWMPEEDLVLVSYIHEHGPTKWRHVPASTGLMRCSKSCRLRWTNYLRPGIRRGNFTPREERVIVHLHSLLGNRWAAIASHLPQRTDNDIKNYWNTHLKKKLVVVEEQHRAAAAAAIVAAAARGHVDDDGVTTSSSPRLLAKDDSYGYGYDARPAAAYPCSMDNVSKLVKVKAGCMKSASSSSPPAQDGSSIRRPFFSLDQTSRSTAPLPPISNVVPQLAGTVLAGQGQGRHGGRFFHEPPQQQLSSIMENWLFLPKHQHQQQMTQSSPTPTAAAHCPCCFS

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGAACAGGGGGACGATCATCGGAGGAAGATGGGCAGCAGGCCGCCATGGTGCGCGTGCGACAGGGCGGGGAATACGGCGGCGATGAAGAAGGGGCCATGGATGCCGGAGGAGGACCTCGTCCTCGTCTCCTACATCCACGAGCACGGCCCCACCAAGTGGCGCCACGTGCCCGCGAGCACGGGGCTGATGCGCTGCAGCAAGAGCTGCCGCCTCCGCTGGACCAACTACCTCCGCCCGGGCATCCGGCGCGGCAACTTCACGCCGCGCGAGGAGCGCGTCATCGTCCACCTCCACTCCCTGCTGGGCAACCGGTGGGCGGCCATCGCGTCGCATCTCCCGCAGCGCACCGACAACGACATCAAGAACTACTGGAACACCCACCTCAAGAAGAAGCTGGTCGTCGTCGAGGAACAGCACCGAGCGGCTGCGGCAGCAGCCATCGTCGCCGCCGCCGCTAGAGGACACGTCGACGACGACGGCGTCACCACCAGTAGTAGTCCCCGCCTGCTCGCCAAGGACGATAGCTACGGCTACGGCTACGACGCGCGCCCTGCTGCAGCCTATCCCTGTAGCATGGACAACGTATCCAAGCTTGTCAAAGTCAAAGCAGGTTGCATGAAGAGCGCATCATCGTCATCCCCGCCGGCGCAGGATGGTAGTAGTATCCGTCGTCCTTTCTTTTCACTCGACCAAACGTCCCGCAGTACGGCGCCGCTGCCACCCATCTCCAACGTGGTGCCGCAGCTAGCCGGTACGGTGTTGGCGGGACAGGGGCAGGGACGACACGGCGGGCGTTTCTTCCACGAGCCGCCCCAGCAGCAGCTGTCCTCAATAATGGAGAACTGGTTATTCCTCCCCAAGCACCAGCACCAGCAGCAGATGACGCAGAGCTCTCCGACTCCGACGGCTGCTGCTCACTGTCCATGCTGTTTTAGC