| TFome pUT5981 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727888 |
| Protein Name | ZmOrphan319 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G176173 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5981
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | P34up |
| Temperature (ºC) | 66.5 |
| Sequence
|
caccATGGGAGGCGAAGGCAGCAC |
3' Primer
| Name | P34dn |
| Temperature (ºC) | |
| Sequence
|
GCTTGTGAGTTGTTCGGAACGAACT |
PCR Condition
Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d
Amino Acid Sequence
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MGGEGSTSPAPGGAACAVCGGAAVVYCVADAAALCSPCDAAVHAANLLASRHERVPLSMAAVPSASGAYDDLFAPDDVDAAAPAQAQGLGSPRNGSSSASFTTSGDSGAEGSSLFDFDLLSGVDLAACVTDGVAPLHHDDAAPLWAQPGLAVAWATAWSPADSAAAVVAAAAEREARVQRYREKRKNRRFHKTIRYASRKAYAEARPRIKGRFVKRAAGTSSSSSSGAGTPDDGNDATGAAAKFWLSFSDDARDGFYVDAGAYGVVPSFYRVRSEQLTS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGAGGCGAAGGCAGCACAAGCCCAGCGCCTGGGGGAGCCGCCTGCGCGGTCTGCGGCGGCGCCGCGGTGGTGTACTGCGTGGCGGACGCTGCGGCGCTCTGCAGCCCCTGCGATGCCGCTGTCCACGCCGCAAACCTGCTGGCGTCGCGCCATGAGCGCGTGCCTCTTTCCATGGCCGCCGTGCCGTCCGCATCAGGCGCGTACGACGACCTGTTCGCGCCCGACGACGTCGACGCGGCGGCGCCCGCGCAGGCGCAGGGGCTGGGCAGCCCCCGGAACGGCAGCTCGTCGGCGAGCTTCACCACCAGCGGCGACAGCGGCGCGGAGGGCAGCAGCCTGTTTGACTTCGACCTGCTGTCTGGCGTGGACCTCGCGGCCTGTGTCACGGACGGCGTGGCGCCCCTCCACCACGACGACGCTGCACCGCTGTGGGCGCAGCCTGGCCTGGCCGTCGCGTGGGCGACCGCGTGGTCTCCGGCGGACTCCGCCGCCGCCGTAGTCGCCGCGGCGGCGGAGCGGGAGGCGAGGGTGCAGAGGTACCGCGAGAAGCGCAAGAACCGCAGGTTCCACAAGACCATCCGCTACGCGTCCCGCAAGGCCTACGCCGAGGCGCGGCCGAGGATCAAGGGCCGCTTCGTCAAGCGCGCCGCCGGCACCTCCTCCTCCTCCTCCTCGGGCGCCGGCACACCCGACGACGGCAACGACGCCACGGGCGCCGCTGCCAAGTTCTGGCTCTCCTTCTCCGACGACGCCAGGGACGGCTTCTACGTGGACGCCGGGGCCTACGGGGTCGTGCCAAGCTTCTACAGAGTTCGTTCCGAACAACTCACAAGC
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