TFome clone pUT5984

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B917_F
Temperature (ºC)	69.5
Sequence Copy to clipboard	caccATGTGGTCGTGGTTGACGAAGATTG

3' Primer

Name	B917_R
Temperature (ºC)
Sequence Copy to clipboard	TGATATGGCGGTTATCCCTGAGAAAC

PCR Condition

PCR Conditions: Volumes per reaction (25l): 5 l GC

Amino Acid Sequence

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MWSWLTKIASACLGPVRRCARTRKDEDDSDNGRGVADDLLWSRDLGRHAAGQFSFAVVQANEALEDHSQVETGSAATFVGVYDGHGGAEAARFISDHLFAHLIRLAQENGTISEDVVRSAFSATEEGFLTLVRRTRFIKPLIAAVGSCCLVGVIWRGTLYVANLGDSRAVIGVLGRSNKIVAEPLTRDHNASREEVRQELISRHPDDSQIVVLKHGVWRIKGIIQVSRTIGDAYLKRREFALDPSITRFRLSEPLRQPILTAEPSIYTRVLNPQDKFIIFASDGLWEHLTNQQAVEIVHSNPRSGIAKRLVRAALKQAARKREMRYDDLRKVEKGIRRFFHDDITVVVVYIDHGLLQERDTSVPELSVRGFVDSVGPSSFSGITAIS

TFome clones do not contain stop codon at 3'end.

CDS

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ATGTGGTCGTGGTTGACGAAGATTGCGTCCGCGTGCTTGGGCCCGGTCCGGAGGTGCGCGCGTACGAGGAAGGATGAGGACGACAGCGATAATGGCCGCGGCGTCGCCGACGACCTGCTGTGGTCGCGGGACCTCGGGCGCCACGCGGCGGGTCAGTTCTCGTTTGCCGTCGTGCAGGCCAACGAGGCGTTGGAGGACCACAGCCAAGTGGAGACAGGCTCTGCCGCAACTTTCGTAGGCGTCTACGACGGTCATGGCGGCGCTGAGGCTGCCCGCTTCATATCTGACCACCTCTTCGCACACCTCATTCGACTTGCTCAGGAAAATGGAACAATATCTGAGGATGTTGTTCGGAGTGCGTTTTCTGCCACCGAGGAAGGCTTCTTGACACTCGTGCGGAGAACACGTTTTATAAAGCCTTTGATTGCTGCGGTTGGATCCTGCTGTCTAGTTGGTGTCATATGGAGAGGGACACTATATGTGGCTAATCTAGGCGATTCCAGGGCTGTTATTGGCGTTCTAGGTAGATCAAACAAGATCGTGGCTGAGCCACTCACAAGAGATCATAATGCAAGTAGGGAGGAGGTCAGGCAGGAGCTTATATCCCGTCATCCAGATGATTCTCAAATTGTCGTTCTCAAGCATGGTGTTTGGCGCATCAAGGGCATAATTCAGGTTTCAAGAACAATTGGAGATGCTTACTTGAAGAGGCGGGAATTTGCTCTTGATCCATCTATCACTCGTTTCCGCCTTTCGGAGCCTCTCCGCCAGCCTATTCTCACAGCAGAGCCATCTATCTATACAAGGGTGCTTAACCCACAAGATAAATTTATTATTTTTGCATCTGATGGTCTTTGGGAGCACTTGACAAATCAACAGGCCGTTGAAATTGTCCACAGCAATCCACGATCAGGAATTGCAAAGAGGCTGGTAAGGGCAGCGTTGAAACAAGCTGCACGGAAGAGGGAAATGAGGTATGATGACCTCAGGAAAGTTGAGAAGGGAATTCGCCGCTTCTTCCACGATGACATTACAGTAGTTGTTGTTTACATAGATCACGGATTACTGCAAGAGAGGGATACTTCCGTGCCAGAGCTCTCAGTCCGTGGCTTTGTTGACTCCGTTGGCCCCTCAAGTTTCTCAGGGATAACCGCCATATCA