| TFome pUT6016 | |
|---|---|
| Template | BT066884.1 |
| Genbank Insert ID | KJ727921 |
| Protein Name | ZmMADS38 |
| Species | Zea mays |
| TF Family | MADS |
| Gene id | GRMZM2G334225 |
| transcript | T03 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6016
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B610_F |
| Temperature (ºC) | 67.2 |
| Sequence
|
caccATGAAGATGCTTGCTAAACTCAAATGTG |
3' Primer
| Name | B610_R |
| Temperature (ºC) | |
| Sequence
|
GCCAATGTTGACCACCCCGG |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MKMLAKLKCEGNIAEQLTPNKEPINSNVEELQQEIKTYQHQMEVLKEQLRMFEPDLTTLMSMNDFETCEKFLMDTLTCVEERKKYLLCNHVAPFDPSASDMQQHVDMFALPPAPPHQQEQQGDDLGGAASAFGGDVSWFADGMPTSSIFAASDPILYFRDQVMFDSTSTMRRDSMVAGVDPGMTSVCQMEQQAPSVDWQQAYTSAEFFSTLIPSTPYPLDDQMQNDMAQVLTSPMVPPHEHDKVEVPMSSSNVPTDGDCGTTLAAAQEDGLPGVVNIG
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAAGATGCTTGCTAAACTCAAATGTGAGGGTAACATAGCCGAACAACTCACACCTAATAAAGAGCCAATAAATTCCAACGTAGAGGAGCTTCAGCAGGAGATTAAGACGTACCAACACCAGATGGAAGTCTTAAAGGAGCAACTTAGGATGTTCGAACCAGATCTGACAACATTGATGTCGATGAACGATTTCGAGACGTGTGAGAAATTCCTCATGGACACCCTCACTTGCGTGGAGGAGCGAAAGAAATATTTGCTGTGCAACCACGTGGCCCCGTTCGATCCGTCGGCCTCCGACATGCAACAACACGTAGACATGTTCGCCCTGCCGCCGGCGCCGCCTCACCAACAGGAGCAACAAGGGGACGACCTAGGAGGCGCCGCCAGCGCGTTCGGTGGCGATGTCTCGTGGTTCGCCGATGGGATGCCGACGTCGTCGATCTTTGCCGCGTCGGACCCAATCTTGTACTTTAGGGATCAGGTGATGTTCGACTCGACGTCGACGATGCGGCGTGACTCGATGGTGGCAGGGGTGGACCCAGGAATGACCTCGGTGTGCCAGATGGAGCAGCAAGCCCCCAGCGTGGACTGGCAGCAGGCGTACACGTCGGCGGAGTTCTTCTCCACGCTGATCCCGTCCACGCCCTACCCCCTTGATGATCAGATGCAGAACGACATGGCTCAAGTGCTTACATCTCCGATGGTGCCACCGCACGAGCACGATAAGGTGGAGGTGCCGATGAGCTCGTCCAACGTGCCGACGGACGGGGATTGCGGCACCACCCTTGCCGCCGCTCAAGAGGACGGCCTGCCCGGGGTGGTCAACATTGGC
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