| TFome pUT603 | |
|---|---|
| Template | EC886470 |
| Genbank Insert ID | HQ858664 |
| Protein Name | ZmMYB154 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G022686 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | Cloned with stop codon. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT603
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C4F UP(EU2) |
| Temperature (ºC) | 74.0 |
| Sequence
|
cAccATGGGGAGGGCGCCGTGCTGCGAGAAGGT |
3' Primer
| Name | C4F DN(EL1) |
| Temperature (ºC) | |
| Sequence
|
tcagaacgagtcggacaggagccaagtttc |
PCR Condition
Amino Acid Sequence
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MGRAPCCEKVGLKRGRWTKEEDQILANYIAEHGEGSWRSLPKNAGLLRCGKSCRLRWINYLRADVKRGNISKEEEDVIIKLHATLGNRWSLIASHLPGRTDNEIKNYWNSHLSRQIHTYRRKYTAGPDTAPIAIAIAIDMSKLHSAERRRGGRTPGRSPKSATSSSSMTKSKQPDDPEPEPEAGGASSPRHSDGARSAVVDPDPDPNQPNSSSGSGSTGEGEDATGPWVLDPIELGDLWEAESEMDALMSIGGAAHDSATAEGFDAAQAQVDDLFDDMDWDNFAAHLWGEPEQQNDHSAQPQPQQAAEPQAAAAADVAAAGAEACDPDEHELEALETWLLSDSF*
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGAGGGCGCCGTGCTGCGAGAAGGTGGGGCTCAAGCGCGGGAGGTGGACGAAGGAGGAGGACCAGATACTCGCCAACTACATCGCAGAGCACGGCGAGGGATCCTGGAGGTCGCTGCCCAAGAATGCAGGGCTGCTCCGCTGCGGCAAGAGCTGCCGCCTGCGGTGGATCAACTACCTTCGGGCCGACGTCAAGAGGGGGAACATCTCCAAGGAGGAAGAGGACGTCATCATCAAGCTCCACGCCACCCTTGGAAACAGGTGGTCCCTGATCGCCAGCCACCTCCCCGGCCGAACAGACAACGAGATCAAGAACTACTGGAACTCGCACCTCAGCCGCCAGATCCACACGTACCGCCGGAAATACACCGCCGGCCCGGACACGGCGCCCATCGCCATCGCCATCGCCATCGACATGAGCAAGCTGCACAGCGCCGAGAGGCGGCGCGGTGGCAGGACCCCGGGCCGGTCGCCGAAGAGCGCCACCAGCAGCAGCAGCATGACCAAGAGCAAGCAGCCGGACGACCCTGAGCCAGAACCGGAGGCCGGCGGCGCGTCCAGCCCGCGGCACAGCGACGGGGCGCGAAGCGCCGTGGTGGACCCGGACCCGGACCCGAACCAGCCCAACAGCAGCAGCGGCAGCGGCAGCACGGGCGAGGGGGAGGACGCGACGGGGCCGTGGGTGCTGGATCCGATCGAGCTCGGGGACCTCTGGGAGGCCGAGAGCGAGATGGACGCCCTCATGTCTATCGGGGGGGCTGCCCACGACTCGGCCACCGCGGAAGGATTCGACGCGGCCCAGGCCCAGGTGGACGACCTGTTCGACGACATGGACTGGGATAACTTCGCGGCCCATCTGTGGGGGGAGCCGGAGCAGCAGAACGACCACAGCGCGCAGCCGCAGCCCCAGCAGGCCGCCGAGCCGCAGGCTGCTGCTGCTGCTGATGTGGCGGCGGCGGGGGCGGAGGCCTGCGACCCGGACGAACACGAACTGGAGGCGTTGGAAACTTGGCTCCTGTCCGACTCGTTCTGA
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