| TFome pUT6092 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727978 |
| Protein Name | ZmNAC78 |
| Species | Zea mays |
| TF Family | NAC |
| Gene id | GRMZM2G406204 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.1824ins65. Last intron called in model incorrect. Introduces stop codons in cloned sequence. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6092
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R108F |
| Temperature (ºC) | 65.6 |
| Sequence
|
caccATGGCGCCGGTCAGTTTGC |
3' Primer
| Name | R108R |
| Temperature (ºC) | |
| Sequence
|
GAAATTATGCCCATTTCTCACCGAGA |
PCR Condition
Amino Acid Sequence
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MAPVSLPPGFRFHPTDEELIIYYLKRKINGRQIELEIIPEVDLYKCEPWDLPEKSFLPSKDLEWYFFSPRDRKYPNGSRTNRATKSGYWKATGKDRKVNSHRRAVGMKKTLVYYRGRAPHGSRTDWVMHEYRLDERECETDTGLQDAYALCRVFKKTAPGPKIIEHYGAVHHPIEHPQWMAEAGSVDRHSPTLDLSSDARGDDFESSSFSFPTEAPSMDSMHGMQMSAPHEDGKWMQFLSEDAFNATNNPFFMNPPSSSSFPCLPSKVDVALECARIQHRLSLPPLEVEDFPQDVSLDTKASVLRSNPNEVDILQEFLSVASASQELINGATSSCYAAELWPSGAGTSSTSTHYVNELSSLVELGVKAKEEADNFYYMDCIVGTSVGFSSKSSVQVDEQAVRLVEIADIEGLKQEEKRQVENLRGVRLHNNDLGEIVVQGDESNPTDCITQYPISDYSADNLGEAGHLADPTDVGGGLATAPIFSQSQPDDFAIGFGDVDVNPNASFDLYERVDVKHGLFVSTAGAPKTFFHHVEPSKKVSVYLSPVASDVVGKATEKFHSPITVTATKASVFSKLKALVTRQGQIPGGEDASIMPPEVLRQQRNSGSE*VAADSTYCCRSS**HQWRQAPRR*IPSKSWSRRRRR**SRGMVVVMETKHVFFFHSPRGAKAFPACSSR*EMGII
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGCCGGTCAGTTTGCCTCCTGGTTTCAGGTTCCACCCCACTGACGAGGAACTCATCATCTACTACCTTAAGAGGAAGATCAACGGGAGACAGATTGAGCTCGAAATCATTCCGGAGGTTGATCTTTACAAGTGCGAGCCATGGGATTTGCCAGAAAAATCATTTCTTCCAAGCAAAGACCTTGAATGGTACTTCTTCAGCCCCCGGGACCGCAAGTACCCGAATGGGTCAAGGACAAACCGTGCAACAAAATCTGGGTACTGGAAGGCAACCGGCAAGGACCGAAAAGTGAACTCGCATAGGCGTGCAGTTGGTATGAAGAAGACCCTGGTGTACTATCGGGGCCGAGCTCCACATGGTTCTCGCACCGATTGGGTCATGCACGAGTACCGCCTCGACGAGAGGGAATGCGAGACTGACACGGGCTTACAGGATGCATACGCTTTATGCCGAGTGTTCAAGAAGACAGCGCCTGGGCCAAAAATCATAGAGCATTATGGCGCAGTGCACCATCCCATCGAACACCCTCAGTGGATGGCAGAGGCAGGCAGTGTTGACCGCCACTCCCCAACGCTGGACTTGTCCAGTGACGCTAGAGGTGACGACTTCGAGAGCAGCAGCTTCTCGTTCCCGACGGAGGCGCCCAGCATGGACTCCATGCACGGGATGCAGATGAGCGCACCCCACGAGGATGGCAAATGGATGCAGTTCCTGAGCGAAGACGCCTTCAACGCCACCAATAACCCTTTCTTCATGAACCCACCTTCTTCTTCAAGCTTCCCATGCCTCCCGTCCAAGGTGGATGTCGCACTGGAGTGTGCAAGGATTCAGCACAGGCTCTCGTTGCCTCCCCTGGAGGTGGAGGACTTCCCACAGGACGTCAGCCTCGACACCAAGGCGAGCGTGCTCCGCAGCAACCCCAACGAGGTCGACATCCTCCAAGAGTTCCTCTCCGTGGCGTCGGCCTCACAGGAGCTCATCAATGGCGCCACCAGCAGCTGCTACGCTGCAGAGTTGTGGCCATCAGGCGCGGGCACAAGCAGCACCAGCACCCACTACGTCAACGAACTGTCTTCCCTCGTTGAGCTCGGGGTGAAGGCCAAAGAGGAGGCAGACAACTTCTACTATATGGACTGCATTGTTGGAACATCTGTAGGGTTCTCATCCAAGTCGTCGGTCCAAGTCGATGAACAGGCCGTTAGGTTGGTTGAGATCGCTGACATCGAGGGGTTGAAGCAGGAAGAGAAGAGGCAGGTAGAGAACCTTAGAGGTGTGCGGCTTCACAACAATGACCTAGGAGAGATTGTTGTACAAGGAGATGAAAGCAATCCAACGGACTGCATCACACAATACCCCATATCAGATTATTCTGCTGACAATTTAGGAGAAGCCGGTCACCTGGCCGATCCCACTGACGTTGGCGGCGGCCTAGCCACTGCACCCATCTTCTCGCAATCTCAGCCTGACGACTTCGCTATTGGGTTCGGTGACGTCGACGTCAACCCTAATGCATCTTTCGACCTGTACGAGAGGGTCGACGTCAAGCATGGGCTCTTCGTCTCAACGGCCGGTGCGCCGAAGACATTCTTCCACCACGTTGAGCCATCGAAGAAGGTCAGCGTCTACCTGAGTCCCGTAGCGAGCGACGTCGTCGGCAAGGCGACCGAGAAGTTCCATTCCCCCATTACTGTGACAGCAACCAAAGCTTCCGTTTTTAGCAAGTTGAAGGCGCTCGTCACTCGTCAGGGACAAATTCCTGGTGGGGAAGACGCCTCCATCATGCCGCCGGAGGTCCTTAGGCAGCAAAGAAATAGCGGCAGTGAGTGAGTTGCTGCAGATAGTACGTACTGTTGTCGCTCCTCTTAATGACACCAATGGAGGCAGGCCCCACGACGATGAATACCGAGCAAGAGTTGGTCAAGAAGGCGAAGAAGGTGATGAAGCCGGGGCATGGTGGTGGTGATGGAAACCAAGCATGTCTTCTTCTTCCACTCTCCAAGAGGAGCAAAGGCATTTCCAGCATGTTCTTCTCGGTGAGAAATGGGCATAATTTC
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