| TFome pUT6127 | |
|---|---|
| Template | BT055755 |
| Genbank Insert ID | KJ728000 |
| Protein Name | ZmGATA4 |
| Species | Zea mays |
| TF Family | C2C2-GATA |
| Gene id | GRMZM2G379005 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G224T p.A82S Present in template FlcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6127
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2844up |
| Temperature (ºC) | 64.5 |
| Sequence
|
caccATGATGAGGGAGCAGAGCCTCCTGG |
3' Primer
| Name | UT2844dn |
| Temperature (ºC) | |
| Sequence
|
ATCCCGGCGGCGGATGAAC |
PCR Condition
Amino Acid Sequence
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MMREQSLLEATCGDLFDHIDDLLDFPKEDSAADVLLLDAPAPGSPLSSARIIDVGRAGSAPAPPALAPSLPAQQDTTAFFASAGPDVFDPRDVGAHLGSCDDIDMDMDMAQLEWLSGLFDDASIPHEPAFPGAAPIIKSSALAAGALLPPDKMEDALLFRSSSPISVLEHGSFNNANGGGGGCSASSESSSASSSSESFSGSGSGSGEGHAWSAPVSPPPVLVIPARARSKRSRPSAFTRAGAEAPTILVPTPMYSSGPSHSDPESIAESSPHPAPPMKKKKKAKKPPAPPAPASSDDNDGDADYEEGGERAEPQGGAVRRCTHCQIEKTPQWRAGPLGPKTLCNACGVRYKSGRLFPEYRPAASPTFVPSIHSNSHKKVVEMRQKAVRSGDPSCDLLQFIRRRD
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGAGGGAGCAGAGCCTCCTGGAGGCGACGTGCGGCGACCTCTTCGACCACATCGACGACCTGCTCGACTTCCCCAAGGAGGACTCGGCCGCCGACGTGCTCCTGCTGGACGCGCCGGCGCCGGGGAGCCCGCTGTCGTCGGCGCGCATCATCGACGTCGGCCGCGCCGGCAGTGCGCCCGCTCCCCCCGCGCTGGCGCCGTCGCTTCCCGCGCAGCAAGACACCACCGCCTTCTTCGCCTCCGCGGGGCCCGACGTGTTCGACCCCAGGGACGTCGGCGCTCACCTCGGCTCGTGCGACGACATTGACATGGACATGGACATGGCGCAGCTGGAGTGGCTGTCGGGGCTCTTCGACGACGCATCCATCCCGCACGAGCCGGCGTTCCCCGGCGCGGCGCCCATCATCAAGAGCTCCGCGCTGGCGGCCGGCGCGCTGCTGCCGCCGGACAAGATGGAGGACGCGCTGCTGTTCCGCAGCTCCAGCCCCATCTCGGTGCTGGAGCACGGCAGCTTCAACAACGCCAACGGCGGTGGCGGCGGCTGCTCCGCGTCGTCGGAGTCGTCCTCGGCGTCCTCGTCCTCGGAGTCTTTCTCCGGCAGCGGGAGCGGGAGCGGCGAAGGCCACGCGTGGTCGGCGCCCGTGTCGCCGCCGCCCGTGCTCGTCATACCGGCGCGCGCGCGGAGCAAGCGCTCCCGCCCGTCCGCGTTCACGCGCGCCGGGGCGGAGGCGCCCACCATCCTCGTGCCGACGCCCATGTACTCGTCCGGGCCGTCCCACTCGGACCCCGAGAGCATCGCTGAGTCCAGCCCGCACCCGGCGCCGCCCATGAAGAAAAAGAAGAAGGCCAAGAAGCCGCCGGCGCCGCCCGCGCCCGCCTCTTCCGACGACAACGACGGCGACGCCGACTACGAGGAAGGCGGCGAGCGCGCGGAGCCGCAGGGCGGCGCCGTCAGGAGGTGCACGCACTGCCAGATCGAGAAGACGCCGCAGTGGCGCGCGGGGCCGCTGGGGCCCAAGACGCTGTGCAACGCCTGTGGCGTGCGCTACAAGTCCGGCCGCCTGTTCCCGGAGTACCGTCCCGCGGCGAGCCCCACCTTCGTGCCGTCCATCCACTCCAACTCGCACAAGAAGGTGGTGGAGATGCGCCAGAAGGCTGTCCGGAGCGGTGACCCGTCGTGCGACCTCCTGCAGTTCATCCGCCGCCGGGAT
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