TFome pUT6134
Template Genomic DNA
Genbank Insert ID KJ728003
Protein Name ZmGRAS40
Species Zea mays
TF Family GRAS
Gene id GRMZM2G368909
transcript T01
Vector pENTR/SD/D-TOPO
Note
Request Information Deposited to ABRC
Subgenome: 1

TFome clone pUT6134

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name Dup18
Temperature (ºC) 61.3
Sequence
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caccATGATAATGGACCCTCGTCCTCGTGAC

3' Primer

Name Ddn18
Temperature (ºC)
Sequence
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GCTAGTCCATGCTGAAAGTGCATATATGA

PCR Condition

Amino Acid Sequence
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MIMDPRPRDDPNLGARLPTPNPPAPHMISFSLQQPQLQFFPSYGFHGHSHSSPVSSHPSPPSAMPGCTTPSPASTTTTEPDSVEDFSETVADDAVLAYINQFLLEDEDEESYPITSAPVEDSALLAAVEKPFVDILESAKPIAAQGYEVKSWITDDCDSTGSGRFRDVVTSSLPREMVREGLVGAAHKGQKNPRDEDMEMEGRKSKQSALCDEETVREMFDKVLLCTDKNCEFHSPMPADAQISGGYVKGSRNKRGRRKGRSGSGAEEEPVDLTTLLIHCAQAAAIDDHRNSSELLKQIRKHSSATGDAGQRLAHYFADGLEARLAGSGSSIYRSLAAKRTSTGDILKAFSLYVKACPFRILSHYVANTTILNATKSATRLHIIDYGIMYGFQWPVLMQRLSKRPGGPPYLRITGIDFPLSGFRPAERVEATGRRLHEYARMFNVPFEYQAIAAKWDTIQVKDLNMKSDEFVVVNCLYRMRNMMDETVTDDSPRTRVLNTIRKLNPHLFVHGIVNGTYNAPFFVTRFKEAMFFFSSIFDMLEANALRMDEHRLLIEREFFGREAVNVIACEGTERIERPETYKQWQMRNLRAGFRQLALDREIMKRARYKVSKSYQGDFLVDEDNKWMLQGWKGRIIYALSAWTS

TFome clones do not contain stop codon at 3'end.

CDS
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ATGATAATGGACCCTCGTCCTCGTGACGATCCCAACCTCGGTGCTCGATTGCCCACCCCAAATCCACCCGCACCTCACATGATTAGCTTCTCCCTCCAGCAGCCACAGCTTCAGTTCTTCCCTTCGTATGGATTCCACGGCCACAGCCACAGCTCTCCTGTCAGCTCGCATCCATCTCCACCCTCGGCCATGCCTGGCTGCACCACGCCAAGCCCGGCATCGACAACCACAACCGAGCCAGACAGCGTCGAGGATTTTTCCGAGACTGTTGCTGACGACGCGGTCCTAGCATACATCAACCAGTTCCTTCTTGAAGACGAGGACGAGGAATCCTATCCTATTACCAGCGCGCCTGTGGAGGATTCAGCACTCCTCGCCGCCGTCGAGAAACCATTCGTCGACATCCTCGAGTCTGCCAAGCCTATCGCAGCACAGGGCTATGAAGTAAAGTCTTGGATCACTGATGACTGTGATTCTACAGGAAGCGGAAGGTTTCGCGATGTGGTCACAAGTAGTCTGCCTCGTGAAATGGTGCGGGAGGGTTTAGTTGGTGCTGCTCATAAGGGTCAGAAGAACCCGCGTGACGAGGACATGGAGATGGAGGGGAGGAAGAGCAAACAGTCGGCACTGTGTGACGAAGAGACTGTCCGGGAGATGTTTGACAAGGTGCTGTTGTGTACCGACAAGAACTGTGAGTTCCACTCACCAATGCCAGCCGATGCACAGATCAGCGGGGGATATGTGAAAGGATCTAGAAATAAGCGAGGGCGAAGGAAGGGGAGATCAGGTTCTGGTGCAGAGGAGGAGCCAGTTGATCTCACAACCCTACTCATACATTGTGCTCAGGCCGCGGCTATCGACGACCACAGGAACTCAAGCGAGCTGCTGAAACAGATCAGGAAGCATTCCTCTGCTACTGGAGATGCTGGCCAGAGATTGGCACACTACTTCGCCGATGGGCTGGAGGCTCGCCTAGCTGGCTCTGGCAGCAGCATCTACCGTTCGCTTGCTGCAAAGAGAACTTCCACTGGTGATATACTGAAGGCGTTCAGTTTGTATGTCAAAGCATGTCCGTTTAGGATACTATCACACTATGTCGCAAACACGACCATCTTAAACGCTACGAAGAGCGCCACAAGGCTGCACATCATAGACTACGGGATAATGTATGGTTTCCAGTGGCCAGTCCTCATGCAGCGGCTCTCAAAGAGACCTGGTGGCCCCCCGTACCTTCGGATCACTGGTATAGACTTTCCCCTGTCAGGATTCCGCCCTGCAGAGCGTGTTGAGGCGACAGGGCGGCGGTTGCATGAGTATGCCCGTATGTTCAATGTCCCATTTGAATACCAAGCCATTGCTGCCAAGTGGGATACTATCCAAGTTAAAGATCTCAACATGAAGAGCGATGAGTTCGTCGTCGTTAACTGCCTCTACCGGATGAGGAACATGATGGACGAGACGGTGACGGATGACAGCCCGAGAACGCGGGTTTTGAACACAATCAGAAAGCTGAATCCCCATCTGTTCGTTCATGGGATCGTCAATGGTACTTACAATGCACCCTTCTTCGTGACGCGGTTTAAGGAGGCTATGTTCTTCTTCTCTTCGATCTTTGATATGCTCGAAGCGAATGCCTTACGGATGGATGAGCATAGGCTGCTGATAGAGAGAGAGTTCTTTGGCCGGGAAGCTGTCAATGTGATTGCCTGTGAGGGCACAGAGAGGATTGAAAGGCCAGAGACCTACAAACAATGGCAGATGAGAAACCTCAGGGCAGGCTTCCGGCAGTTAGCTCTGGACAGGGAGATAATGAAGAGAGCAAGGTACAAGGTAAGCAAGAGCTATCAGGGGGATTTCCTCGTGGACGAAGATAACAAGTGGATGCTACAAGGTTGGAAGGGTCGTATCATATATGCACTTTCAGCATGGACTAGC


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