| TFome pUT6195 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728049 |
| Protein Name | ZmCOL12 |
| Species | Zea mays |
| TF Family | C2C2-CO-like |
| Gene id | GRMZM2G041991 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6195
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R171F |
| Temperature (ºC) | 66.2 |
| Sequence
|
caccATGAGCAGCTCCAAGCACGCC |
3' Primer
| Name | R171R |
| Temperature (ºC) | |
| Sequence
|
ACGACCTCGACACTCACGGGGACA |
PCR Condition
Amino Acid Sequence
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MSSSKHAAGAVGGKAARACDSCLRRRARWYCAADDAFLCQGCDASVHSANPLARRHERLRLRPTDPHSTTLEAGVATATWKKRQQQVAPAWSKRKARTRRPHVKSVGELLSRKLVVVPEVSPIESSEERKAEEEEEEEEGQLLYCVPTFDRALAELCSPPPPVDDPTASSCCRDDVDGAVENNTKAPPVVVAESPVQQLPDSFAGFGPTDAELREFAADMEALLGQGLDDGNELDRSFYMESLGLMAQQAEDVGRIKMEPNGIVSSRSRGEGAPGFGPTEMKPEASSAAAEVLDTDFNCCSPTVMMDNEDEDSFEQKASASNAAAAAAGTQFLKRSLDLSLNYEAIIESWGSSPWTDGQRPSVQLDDFWPHAHLTGWMAGGGRLGGEAAVTPRLGMGGGREARVTRYREKRRTRLFAKKIRYEVRKLNAEKRPRMKGRFVKRPAAAAGGGGEELPLPPRAPSPSLPIEVARVAAQSPCGVPIGVRPRPCPRECRGR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAGCAGCTCCAAGCACGCCGCGGGCGCCGTAGGCGGCAAGGCCGCGCGCGCCTGCGACAGCTGCCTGAGGCGGCGCGCGCGGTGGTACTGCGCTGCCGACGACGCCTTCCTGTGCCAGGGCTGCGACGCCTCGGTGCACTCGGCGAACCCGCTCGCCAGGCGGCACGAGCGCCTGCGCCTGCGCCCGACGGACCCGCACTCGACGACGCTCGAGGCCGGCGTGGCGACGGCGACGTGGAAGAAGCGCCAGCAGCAGGTCGCGCCGGCGTGGTCCAAGCGCAAGGCGCGCACTCGGAGGCCGCACGTCAAGAGCGTCGGGGAGCTCCTGTCGAGGAAGCTCGTCGTCGTGCCTGAGGTGTCCCCCATCGAATCGTCGGAGGAGCGGAAGGCCGAGGAGGAGGAGGAAGAGGAGGAGGGGCAGCTGCTGTACTGCGTGCCGACCTTTGACCGCGCCCTGGCCGAGCTCTGCTCGCCACCGCCTCCGGTCGATGACCCGACGGCCTCATCCTGCTGCAGGGATGACGTCGACGGCGCCGTGGAGAACAACACAAAGGCACCACCGGTCGTGGTCGCGGAATCGCCCGTGCAGCAGCTCCCGGACAGCTTTGCCGGCTTCGGCCCCACGGACGCCGAGCTCAGAGAGTTCGCCGCAGACATGGAAGCCCTTCTTGGCCAGGGCCTGGACGACGGCAACGAGCTGGATAGATCGTTCTACATGGAGAGCCTAGGGCTAATGGCGCAGCAGGCGGAAGACGTCGGCCGGATAAAGATGGAGCCCAACGGCATCGTAAGCTCCCGCTCCCGCGGCGAGGGCGCTCCCGGGTTTGGCCCTACGGAGATGAAGCCAGAGGCGTCGTCAGCTGCTGCTGAGGTGCTGGACACAGACTTCAACTGCTGCTCGCCCACGGTGATGATGGATAACGAGGATGAGGACAGTTTCGAACAGAAGGCGTCGGCGAGCAACGCTGCCGCCGCCGCCGCGGGTACGCAATTCTTGAAGAGGAGCCTGGATCTCAGCCTCAACTACGAGGCCATCATCGAGAGCTGGGGAAGCTCGCCGTGGACCGATGGCCAACGGCCAAGCGTCCAGCTCGACGATTTCTGGCCCCACGCCCACCTCACGGGCTGGATGGCGGGAGGAGGACGGTTGGGTGGGGAGGCGGCGGTGACTCCACGGCTGGGGATGGGCGGCGGGCGAGAGGCGCGGGTAACACGGTACCGGGAGAAGCGGCGGACGAGGCTGTTCGCCAAGAAGATAAGGTACGAGGTCCGGAAGCTCAACGCGGAGAAGCGGCCGCGGATGAAGGGCCGATTCGTCAAGCGACCAGCTGCCGCTGCAGGAGGAGGAGGCGAGGAGCTACCATTGCCGCCCCGTGCGCCGTCACCTAGCTTGCCGATCGAAGTCGCCCGTGTCGCGGCGCAGTCCCCGTGCGGCGTGCCGATCGGGGTCAGGCCGCGCCCGTGTCCCCGTGAGTGTCGAGGTCGT
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