TFome clone pUT6436

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	CD0072_F
Temperature (ºC)	77.4
Sequence Copy to clipboard	caccATGTCGACGGCGCTGGCGGTGGTCG

3' Primer

Name	CD0072_R
Temperature (ºC)
Sequence Copy to clipboard	CCCAGCTGGGTTATTTGCATCCCGT

PCR Condition

5 l GC buffer (Thermo Fisher) 1.25 ul DMSO 100% .5

Amino Acid Sequence

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MEAFAAQHQGDVCMEAVGVDTAAQQGPRPRTAHRRSRSDVPSGYYFGGEGHQLPPPKVEAGWGGHLHGGGGDDDLFNAYLNLEGLDGLNSSDDRRQYYDGDSRGSSVRTNGADSSENDSEECAAAGPPVAHHVRSLSMDSSLIGKLNFSAGAAAANGGVIPGPNRFSLEFGSGEFTPVEMNKIMADEKLAEMALADPKRAKRVLANRQSAARSKERKMRYIAELEQKVQILQTEATALSAHLTLLQRDSAGIATQNNELQFRLQAMAQQAQLRDALNEALTAEVQRLKLATAELGDSCSSNSLAQQMQIQLSAHDQTFELHQQQAMPIAFYQLRQAQ

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGAGGCGTTCGCGGCCCAGCATCAGGGGGACGTGTGCATGGAGGCCGTCGGCGTCGACACCGCGGCGCAGCAGGGCCCGCGCCCGCGCACGGCGCACCGGCGCTCCCGCAGCGACGTCCCGTCCGGGTACTACTTCGGCGGCGAGGGGCACCAGCTGCCGCCGCCCAAGGTGGAGGCCGGATGGGGCGGGCACCTCCACGGCGGCGGGGGCGACGACGACCTCTTCAACGCGTATCTCAACCTGGAAGGGCTGGACGGGCTGAACTCCTCCGACGACCGCCGGCAGTACTACGACGGCGACAGCCGCGGGAGCAGCGTGAGGACCAACGGCGCCGACAGCAGCGAGAACGATTCGGAGGAGTGCGCCGCCGCAGGCCCGCCCGTGGCCCACCACGTCAGGAGCCTGTCCATGGACAGCAGCCTCATCGGCAAGCTCAACTTCTCCGCCGGCGCGGCTGCGGCCAACGGCGGCGTCATTCCGGGGCCCAACAGGTTCAGCCTCGAGTTCGGCAGCGGCGAGTTCACCCCCGTCGAGATGAACAAGATCATGGCCGACGAGAAGCTGGCCGAGATGGCGCTCGCCGACCCTAAGCGCGCCAAGAGGGTGCTGGCCAACAGGCAGTCGGCGGCGCGGTCCAAGGAGCGGAAGATGCGATACATCGCGGAGCTGGAGCAGAAGGTGCAGATCCTGCAGACGGAGGCCACCGCTCTCTCCGCCCACCTCACGCTTCTTCAGCGGGACTCCGCGGGGATCGCGACGCAGAACAACGAGCTCCAGTTCCGGCTGCAGGCCATGGCGCAGCAGGCGCAGCTGCGTGACGCATTGAATGAGGCACTGACAGCCGAGGTCCAGCGCCTGAAACTCGCGACTGCAGAGCTCGGCGACTCTTGCTCGTCCAACAGTCTAGCCCAGCAGATGCAGATCCAACTCAGCGCGCATGACCAGACGTTCGAACTGCATCAGCAGCAGGCGATGCCGATAGCGTTCTACCAGCTGCGGCAGGCGCAG