Tfome Clone Information

TFome Information for pUT6465

TFome Name pUT6465
Template RT-PCR
Genbank Insert ID KJ728191
Protein Name ZmMYB77
Species Maize
TF Family MYB
Gene ID GRMZM2G077147
transcript T01
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: CD0150_F 3' Primer Name: CD0150_R
5' Primer Tm (º C): 76.3 3' Primer Tm (º C):
5' Primer Sequence: caccATGTGCACGAGGGGCCACTGGAGGC 3' Primer Sequence: CCGGCGGCGATGGGAAGCCCCAACC
PCR Condition: PCR Conditions: Volumes per reaction (30l): 6 l GC
Nucleotide Sequence: ATGTGCACGAGGGGCCACTGGAGGCCGTCGGAGGACGAGAAGCTGAAAGAGCTCGTTGCGCTGTACGGGCCGCACAACTGGAACGCCATCGCCGAGAAGCTTCAAGGGAGATCAGGGAAAAGCTGCAGGCTGCGGTGGTTCAACCAGCTGGACCCGAGGATCAACCGGAGCCCCTTCTCGGAGGAGGAGGAGGAGCTGCTCCTCGCCTCCCACCGCGTCCACGGCAACCGCTGGGCCGTCATCGCCAGGCTGTTCCCGGGGCGCACCGACAACGCCGTCAAGAACCACTGGCATGTGATCATGGCGCGGCGGTGCAGGGAGCGGATGCGGCTGTCGAACAGGCGTGCCGGCGGCGCTGCTGCTGCTGGTGCTGGTGCCGTCGTCGTCGCCACCGGTGCTGCTGCCGAAGATGGCAGTAACCCTAGGAGCGCCAATAAGAAGCCGAGGCCTGACGCGAGCGGCATGGCCTCCTTGCTAGACAAGTACCGAAGAGAATTTTCCGCCGTCCCGTTTGCCATTAACCATGATAGCAATCAGCAGCAGGGCTATTGTTCAAATACTAACGAAGACGCAAACAAATCAGTCGAATTCTACGATTTCCTCCAAGTGAACGCGAACTCGAGCGACACCAAGTGTGGTTCGAGCATTGAGGAGCAAGAGGCGGAGAGCCGGGATGACGATCAAGCCGAAGGGCAGGTGCAGTTCATAGATTTCCTGGAGGTTGGGGCTTCCCATCGCCGCCGG
TFome clones do not contain stop codon at 3'end.
translation: MCTRGHWRPSEDEKLKELVALYGPHNWNAIAEKLQGRSGKSCRLRWFNQLDPRINRSPFSEEEEELLLASHRVHGNRWAVIARLFPGRTDNAVKNHWHVIMARRCRERMRLSNRRAGGAAAAGAGAVVVATGAAAEDGSNPRSANKKPRPDASGMASLLDKYRREFSAVPFAINHDSNQQQGYCSNTNEDANKSVEFYDFLQVNANSSDTKCGSSIEEQEAESRDDDQAEGQVQFIDFLEVGASHRRR