| TFome pUT6471 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728197 |
| Protein Name | ZmMYBR29 |
| Species | Zea mays |
| TF Family | MYB-related |
| Gene id | AC199820.4_FG006 |
| transcript | T006 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A46G p.M16V; c.C266T p.P76S; c.T253A p.L85M None present in functional domains. SNPs present in HapMap2 PZE02206978003; p.76 PZE02206977823; p.85 PZE02206977796 |
| Request Information | Deposited to ABRC |
TFome clone pUT6471
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0171_F |
| Temperature (ºC) | 69.8 |
| Sequence
|
caccATGGATCCCATGTTCAGTAGCGAGT |
3' Primer
| Name | CD0171_R |
| Temperature (ºC) | |
| Sequence
|
CCTACGGAAGAACTTCTGTGCATGG |
PCR Condition
PCR Conditions: Volumes per reaction (30l): 6 l GC
Amino Acid Sequence
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MDPMFSSEWSVSEIKMAKSLIASRNTSSNLASDDDASNKKHSDIVDELQARFPWKGKHEVIGLYVDLTMEMINTPPEVGDDGLVLDDDNFGMPVEEQPQMKYTGRFWTREEHRNFLRGLNMYGRGNWMYISRDFVPTKTPMQIYSHAQKFFRR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGATCCCATGTTCAGTAGCGAGTGGAGTGTCTCCGAGATCAAGATGGCAAAATCACTCATCGCTAGCCGCAACACCAGCAGCAACTTAGCTAGCGACGACGACGCAAGCAACAAGAAGCACAGCGACATCGTGGACGAGCTCCAAGCAAGGTTCCCATGGAAGGGCAAGCACGAGGTAATTGGCTTGTATGTTGACCTCACGATGGAGATGATAAATACACCGCCTGAGGTGGGAGATGACGGACTTGTGTTGGACGACGACAATTTTGGGATGCCAGTGGAGGAACAACCACAGATGAAATACACTGGGAGGTTTTGGACCAGGGAGGAGCACAGGAATTTCCTCCGTGGCCTGAACATGTATGGTCGTGGTAACTGGATGTACATCTCTAGGGACTTCGTCCCCACTAAGACGCCAATGCAGATCTATAGCCATGCACAGAAGTTCTTCCGTAGG
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