TFome clone pUT6472

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	CD0186_F
Temperature (ºC)	71.1
Sequence Copy to clipboard	caccATGACGATGGCGATGGCAAGTATGC

3' Primer

Name	CD0186_R
Temperature (ºC)
Sequence Copy to clipboard	AAGCAGATCACTGTAATCGAAGTAGT

PCR Condition

PCR Conditions: Volumes per reaction (30l): 6 l GC

Amino Acid Sequence

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MTMAMASMPPACAATELVAKGRESAAVLKAMLGQLPAAATPHDGLRDLAEQVLRCCDRALAALLRGAATEEAACSSSARKRRKQPAERQGGLAPPPPSPAATGSKRMRMSRGERGTRAEKKETMEDGFIWRKYGQKDIHGSNYPRLYFRCTYKDDRGCEARRQVQRSDTDPCAAYLITYFGEHTCCCRDDAVEAPAPFVIDFGLSAAACDDGLQLPQYGSPWPSCDDDGPGPVELQTPPPRTSADLCSSPPEEELRAGACDVAEFVAEQSTTVTAELMGRMTSPEWDGCLDWELGEGDSSLDVDGFDRYYFDYSDLL

TFome clones do not contain stop codon at 3'end.

CDS

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ATGACGATGGCGATGGCAAGTATGCCGCCGGCTTGTGCGGCAACCGAGCTGGTGGCCAAGGGGAGGGAGTCGGCTGCCGTTCTCAAGGCCATGCTCGGCCAGCTTCCTGCGGCGGCGACGCCCCATGATGGTCTCCGGGATCTGGCCGAGCAGGTCCTCCGCTGCTGCGACCGAGCTCTGGCGGCCTTGCTCAGAGGCGCGGCAACGGAGGAGGCTGCCTGCAGCAGTTCGGCCAGGAAGCGCCGCAAGCAGCCAGCAGAGCGGCAGGGCGGCCTGGCTCCTCCTCCTCCTTCTCCTGCTGCGACGGGATCCAAGAGGATGAGGATGAGCCGTGGAGAGCGGGGGACACGAGCGGAGAAGAAAGAAACAATGGAGGACGGCTTCATCTGGAGGAAGTACGGCCAGAAGGACATCCACGGCAGCAACTACCCCAGGCTCTACTTCCGCTGCACCTACAAGGACGACCGCGGCTGCGAGGCCCGGAGGCAGGTGCAGCGGTCGGACACCGACCCCTGCGCGGCCTACCTCATCACCTACTTCGGCGAGCACACCTGCTGCTGCCGCGACGACGCCGTCGAGGCGCCAGCGCCATTCGTCATCGACTTCGGCTTGAGCGCCGCTGCCTGTGACGACGGCCTGCAGCTGCCGCAATACGGTTCGCCTTGGCCGTCTTGCGACGACGACGGCCCGGGCCCGGTGGAATTGCAAACGCCGCCGCCGCGCACATCAGCTGATCTGTGCAGTTCACCGCCGGAGGAGGAGTTGCGGGCTGGCGCGTGCGACGTGGCTGAGTTCGTCGCCGAGCAGTCAACGACGGTGACAGCAGAGCTCATGGGTAGGATGACCTCGCCGGAGTGGGACGGGTGCTTGGACTGGGAGTTGGGCGAAGGCGACAGCTCACTCGACGTTGACGGTTTCGACAGATACTACTTCGATTACAGTGATCTGCTT