| TFome pUT6487 | |
|---|---|
| Template | BT038466.1 |
| Genbank Insert ID | KJ728210 |
| Protein Name | ZmMYB30 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G171781 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A371G p.N124S; c.T627C p.H209(=); c.G726A p.A242(=); c.C1009A p.P337T PZE08127729220 c.T1056A p.V352(=) PZE08127729267 c.G1092A p.T364(=); c.A1160G p.N387S PZE08127729371\n c.A1164C p.I388(=) present in template FlcDNA. None present in Myb-domains. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6487
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B305_F |
| Temperature (ºC) | 72.0 |
| Sequence
|
caccATGGGGAGGCATTCTTGCTGCTACA |
3' Primer
| Name | B305_R |
| Temperature (ºC) | |
| Sequence
|
GATATTCTCAAAAGATAGTTGCATTCTTT |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MGRHSCCYKQKLRKGLWSPEEDEKLMNHITKHGHGCWSSVPKLAGLQRCGKSCRLRWINYLRPDLKRGAFAQDEEDLIIELHAVLGNRWSQIAAQLPGRTDNEIKNLWNSCIKKKLRQKGIDPSTHKPLTEADRGGAAPTISTERTSGSSDVNPSSTGPLGNLSPLLSETAQSSMLMPVYDKNRAETPNLARPKLPPKELFLEQLTAGHESPSTCHSSGQTLYFPFQQPLGYNSESGSSDGANMNSLWFNQSDFNCSTISTVMPPVSPSALSTSMGLNLPPDNPRHGGTGIGSTAVDSFYWDGTNPSSSSSTGSRGSNSMGFEPQSTSTILENSVFTWTDIGQEKDTRAHLVAELKWPDSFAETTTAMQNQSQTLYDDVIKAESQFSIEGICASWFQNQQPQQQLQVAPDMYDKDLQRMQLSFENI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGAGGCATTCTTGCTGCTACAAGCAGAAGCTGAGAAAGGGGCTCTGGTCCCCTGAGGAGGATGAGAAACTCATGAATCACATAACAAAGCATGGCCATGGCTGCTGGAGCTCTGTTCCCAAACTCGCTGGGCTTCAAAGGTGCGGAAAGAGTTGTAGGCTGAGGTGGATAAACTATCTGAGGCCAGACCTCAAGAGAGGGGCATTCGCCCAAGACGAAGAAGACCTCATCATCGAGCTGCACGCTGTCCTCGGGAACAGGTGGTCTCAAATTGCAGCGCAGCTGCCAGGGAGGACTGATAATGAGATCAAGAATCTATGGAACTCGTGCATCAAGAAGAAGCTGAGGCAGAAAGGCATTGATCCCAGCACCCACAAGCCACTTACTGAGGCTGATCGCGGCGGTGCAGCTCCCACCATCAGCACCGAGAGAACCTCAGGGTCCAGCGACGTCAACCCATCAAGCACTGGTCCTCTGGGGAACTTGAGCCCCCTCCTCAGCGAGACGGCTCAATCATCAATGCTGATGCCCGTGTACGACAAGAATCGTGCTGAAACACCAAACTTGGCACGCCCTAAGCTGCCACCAAAGGAGCTGTTCCTGGAGCAGCTTACTGCTGGACACGAGAGTCCATCAACCTGCCACTCATCAGGCCAAACTCTCTATTTCCCTTTCCAACAGCCTTTAGGCTACAACAGTGAGAGTGGCAGCAGCGATGGGGCAAATATGAATTCACTCTGGTTCAACCAGAGTGACTTCAACTGCAGCACAATTTCTACAGTGATGCCGCCAGTTTCACCATCGGCCCTCTCTACATCAATGGGGCTGAATCTTCCACCTGATAATCCTCGACATGGAGGAACTGGCATTGGAAGTACCGCAGTAGATTCATTCTACTGGGATGGTACTAATCCTAGCAGCAGTAGCAGTACTGGGAGCAGAGGGAGCAACAGTATGGGATTTGAACCACAGAGCACGAGCACAATTCTGGAGAACAGTGTCTTCACATGGACAGACATTGGACAAGAAAAAGATACAAGGGCTCACCTAGTAGCGGAACTTAAGTGGCCTGACTCCTTTGCCGAGACAACCACAGCCATGCAGAACCAGAGCCAAACGCTTTATGATGATGTGATCAAGGCAGAGAGCCAATTCAGCATCGAAGGGATATGTGCTTCTTGGTTCCAGAATCAGCAGCCACAGCAACAGCTGCAGGTAGCACCAGACATGTATGACAAGGACTTGCAAAGAATGCAACTATCTTTTGAGAATATC
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