| TFome pUT6488 | |
|---|---|
| Template | BT069847.1 |
| Genbank Insert ID | KJ728211 |
| Protein Name | ZmEREB60 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G131266 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G236T c.C237T p.G79V; c.A238T c.T239A c.C240T p.I80Y; c.G241C c.C242T c.A243C p.A81L Present in functional domain. Present in template FlcDNA BT069847.1. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6488
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B394_F |
| Temperature (ºC) | 73.0 |
| Sequence
|
caccATGGCCAGGCCCCGCAA |
3' Primer
| Name | B394_R |
| Temperature (ºC) | |
| Sequence
|
CTCGTACAGGTCGAAGTCGAACATTGGC |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MARPRKNGGTDEDDANAATGATGKPKKLMKRARRKSESPSPRSSAYRGVTRHRWTGRFEAHLWDKDARNGSRSKKGKQVYLGAYDDEDAAARAHDLAALKYWGPAGTVLNFPLSGYDEERREMEGQPREEYVASLRRRSSGFARGVSKYRGVARHHHNGRWEARIGRVLGNKYLYLGTYATQEEAAVAYDMAAIEHRGFNAVTNFDISHYINHWHRHCHGPCDGSLGAMDVAPNVSLELDLLECPATVGLGLEETTGDDEFHNREDYLGHLFGVQQLPDEMGPPAHQMAPASSALDLVLQSPRFKELMQQVSAAGASETNGGSMRSSPSTSLCSFSPSPLELPSPPLQQPTEFIDGAPPRCSFPDDVQSFFDFKNDNDMSFVYAEVDTFLFGDLGAYAPPMFDFDLYE
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCAGGCCCCGCAAGAACGGCGGTACCGACGAGGATGATGCTAACGCCGCCACGGGAGCCACCGGGAAGCCGAAGAAGCTGATGAAGCGGGCGCGGAGGAAGAGCGAGAGCCCCTCCCCGCGCAGCTCCGCGTACCGCGGCGTCACACGGCACCGGTGGACGGGGCGGTTCGAGGCGCACCTGTGGGACAAGGACGCCCGCAACGGCTCCCGGAGCAAGAAAGGCAAGCAAGTTTATCTCGGCGCGTACGACGACGAGGACGCGGCGGCGCGCGCGCACGACCTGGCGGCGCTCAAGTACTGGGGCCCCGCCGGCACCGTCCTCAACTTCCCGCTGAGCGGCTACGACGAGGAGCGGAGGGAGATGGAGGGGCAGCCCCGGGAGGAGTACGTCGCGTCGCTGCGGCGGCGGAGCAGCGGCTTCGCGCGAGGGGTGTCCAAGTACAGGGGCGTTGCCAGGCATCATCACAACGGGAGATGGGAGGCGAGGATCGGGCGCGTGTTAGGCAACAAGTACCTCTATCTCGGCACTTACGCAACACAAGAGGAGGCGGCAGTGGCGTATGACATGGCGGCTATAGAACACCGCGGCTTCAACGCGGTCACCAACTTCGACATCAGCCATTACATCAACCACTGGCATCGACATTGCCATGGCCCCTGCGACGGCAGCCTCGGCGCGATGGACGTCGCACCGAACGTCTCGCTCGAACTAGACCTACTCGAGTGCCCGGCCACCGTCGGCCTCGGCCTCGAGGAGACCACGGGCGACGATGAATTCCACAACCGCGAGGACTACCTGGGGCACCTTTTCGGCGTGCAGCAGCTTCCCGACGAGATGGGGCCGCCGGCTCATCAGATGGCACCAGCATCGTCGGCGCTCGATCTGGTGTTGCAGTCGCCTAGGTTCAAGGAGCTGATGCAACAGGTTTCCGCGGCGGGAGCCTCCGAGACCAACGGCGGCAGCATGCGTAGCTCGCCATCAACGTCCCTGTGTTCGTTCTCGCCTTCGCCTCTGGAGCTGCCATCGCCGCCGCTACAGCAACCTACTGAATTCATCGATGGCGCGCCGCCGCGGTGCAGCTTCCCGGATGACGTGCAGTCGTTCTTCGATTTCAAGAACGATAACGACATGAGCTTCGTCTACGCGGAGGTTGACACATTCCTCTTCGGCGACCTAGGCGCATACGCTCCGCCAATGTTCGACTTCGACCTGTACGAG
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