| TFome pUT6496 | |
|---|---|
| Template | BT040338 |
| Genbank Insert ID | KJ728219 |
| Protein Name | ZmOrphan250 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G412937 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6496
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C515_F |
| Temperature (ºC) | 71.2 |
| Sequence
|
caccATGGTTTACGATGGCGCCGTGAAG |
3' Primer
| Name | C515_R |
| Temperature (ºC) | |
| Sequence
|
GAGACGAGGCGAGGAAGGGGAA |
PCR Condition
PCR Conditions: Volumes per reaction (25l): 5 l GC
Amino Acid Sequence
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MVYDGAVKDQDQETVAASTASAASVVAVSEASPAPVVVVVSARPSARPPHDKRLGVRHPLKHRWFRAGGKMMMEPGGVLPAHAHEEEEEGASEVEEEELVEAEASSAETVTWGAEVEVSSAPAAGVQEMNAEVEGGEMEVSPEPAVAVGGTELEPQQDDEDEVSSVVLARGERKQEAAPASAVLAVEAPMEMGQDEERAEKEKRGKERERQKERERVDEVGYMSGGWKSVDGTLNCGYSSFRGRRASMEDFYDIKASKIDDKQINLFGIFDGHGGSRAAEYLKEHLFENLMKHPEFMTNTKLALSETYRKTDSEFLDAERNTHRDDGSTASTAVMVADHLYVANVGDSRAVISKAGKAIALSEDHKPNRSDERNRIESAGGIVMWAGTWRVGGVLAMSRAFGNRLLKQFVIADPEIQEQEINEELEFLIIASDGLWDVVSNEDAVTLVKMEEEPEAAARKLTETAFSRGSGDNITCIVVKFQHDKPRSGGGDSPSSPRL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGTTTACGATGGCGCCGTGAAGGACCAGGACCAGGAGACCGTGGCGGCGAGCACTGCATCGGCGGCATCGGTGGTGGCGGTGTCGGAGGCGTCTCCCGCGCCGGTGGTGGTGGTGGTCAGTGCGAGGCCCTCGGCGAGGCCGCCGCACGACAAGCGCCTCGGCGTGCGCCACCCCTTGAAGCACCGCTGGTTTCGTGCTGGTGGGAAGATGATGATGGAGCCCGGAGGGGTGCTGCCTGCTCACGCGCATGAGGAGGAGGAGGAGGGAGCCAGCGAGGTGGAAGAGGAGGAGTTAGTGGAGGCGGAGGCCTCGTCCGCGGAGACGGTGACGTGGGGCGCTGAAGTGGAGGTGTCGTCCGCGCCGGCGGCAGGGGTGCAGGAGATGAACGCGGAGGTGGAGGGTGGCGAAATGGAGGTGTCGCCTGAGCCTGCGGTCGCTGTGGGGGGCACCGAGTTGGAGCCCCAGCAGGACGACGAGGACGAGGTCTCGTCCGTCGTGTTGGCGCGAGGGGAGAGGAAGCAGGAGGCGGCTCCTGCTTCTGCGGTGCTAGCTGTGGAGGCGCCCATGGAAATGGGTCAGGATGAGGAGAGGGCGGAGAAGGAGAAGAGGGGCAAGGAGAGGGAAAGGCAGAAGGAGCGGGAGAGGGTGGATGAGGTCGGCTACATGAGCGGAGGATGGAAGAGTGTTGATGGAACACTGAATTGTGGATATTCAAGTTTTAGAGGAAGAAGGGCAAGCATGGAAGATTTCTATGATATAAAAGCATCTAAAATTGATGATAAACAAATAAATCTCTTCGGAATTTTTGATGGTCATGGTGGTTCACGTGCTGCTGAGTATTTGAAGGAGCACTTGTTTGAGAACCTCATGAAACATCCGGAGTTCATGACAAATACAAAATTAGCATTAAGTGAAACATATAGAAAGACCGATTCAGAGTTTTTGGATGCTGAAAGGAATACTCATAGAGATGATGGGTCAACGGCATCGACAGCAGTGATGGTTGCTGATCATCTTTACGTGGCAAATGTTGGAGACTCGAGGGCTGTTATATCAAAGGCTGGCAAAGCTATTGCGCTCTCTGAAGATCACAAGCCAAACAGAAGTGATGAGAGGAATCGAATTGAGAGTGCTGGTGGGATAGTTATGTGGGCAGGAACTTGGAGGGTAGGTGGTGTACTTGCGATGTCTCGGGCATTTGGCAACCGTCTCTTAAAACAATTTGTTATTGCAGACCCTGAAATTCAGGAACAAGAAATAAATGAGGAGTTGGAGTTTCTAATTATAGCTAGTGATGGGCTGTGGGATGTGGTTTCGAATGAGGACGCTGTTACACTTGTGAAAATGGAGGAGGAACCAGAGGCTGCAGCTCGGAAGCTGACAGAGACCGCGTTCTCTCGCGGGAGCGGCGATAACATCACGTGCATCGTGGTGAAGTTCCAGCATGACAAGCCGCGCAGTGGCGGTGGCGATTCCCCTTCCTCGCCTCGTCTC
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