| TFome pUT6530 | |
|---|---|
| Template | BT088320.1 |
| Genbank Insert ID | KJ728253 |
| Protein Name | ZmMYB158 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G078820 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C281A; C843A. p.P94H; Both SNPs not present in template FlcDNA. Present in Myb domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6530
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C151_F |
| Temperature (ºC) | 76.4 |
| Sequence
|
caccATGGGCGCGGAGGCGGCG |
3' Primer
| Name | C151_R |
| Temperature (ºC) | |
| Sequence
|
CTGCATCCGGCCGACCTGTTCGGC |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MGAEAAERDRIRGPWSPEEDEALRRLVERHGARNWTAIGRGVPGRSGKSCRLRWCNQLSPRVARRPFTADEDAAIARAHARLGNRWAAIARLLHGRTDNAVKNHWNCSLKRKLGAAAAASGSAVSADDSAAAAETEARRGKMMMMSISPPDSPSGSGSGSDRSDLSSHGAGSGSGSGSGQIYRPVAARSGGFEPADCAMSRPQEEDPLTALSLSLPGTDQRFHHHSAAADSHFQELPPSPSSPAPSAYPFSPEFAAAMQQLIRTEVQRYMASAGLRAGCGAVGATDLCMPQLVDGVMRAAAEQVGRMQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGCGCGGAGGCGGCGGAGCGCGACAGAATCAGGGGCCCGTGGAGCCCCGAGGAGGACGAGGCGCTGCGGCGGCTGGTGGAGCGGCACGGCGCGCGCAACTGGACGGCGATCGGGCGCGGCGTCCCGGGCCGGTCGGGCAAGTCGTGCCGCCTGCGGTGGTGCAACCAGCTGTCGCCAAGGGTGGCGCGCCGCCCCTTCACGGCCGACGAGGACGCGGCCATCGCCCGCGCCCACGCCCGCCTCGGCAACCGCTGGGCCGCCATCGCGCGCCTGCTCCACGGCCGCACCGACAACGCCGTCAAGAACCACTGGAACTGCTCCCTCAAGCGCAAGCTCGGCGCCGCCGCCGCCGCGTCCGGTTCGGCGGTTTCCGCTGACGACTCCGCCGCCGCCGCCGAGACCGAGGCGCGCCGGGGCAAGATGATGATGATGAGCATCTCGCCGCCCGACAGCCCCTCGGGGTCGGGGTCGGGGTCCGACCGTAGCGACCTCAGCAGCCACGGCGCCGGGTCCGGGTCCGGGTCCGGGTCCGGCCAGATTTACCGCCCCGTGGCCGCGCGCTCCGGCGGGTTCGAGCCGGCGGACTGCGCCATGAGCCGTCCGCAGGAGGAGGACCCGCTCACCGCCCTCAGCCTCTCGCTTCCCGGCACGGACCAGCGGTTTCACCACCACAGCGCCGCCGCCGACAGCCACTTCCAGGAGCTCCCGCCGTCGCCCTCCTCACCCGCGCCCTCGGCCTACCCGTTCAGCCCCGAGTTCGCGGCGGCGATGCAGCAGCTGATCCGCACCGAGGTGCAGCGGTACATGGCGAGCGCGGGCCTGCGCGCCGGCTGCGGCGCAGTTGGCGCGACCGACCTCTGCATGCCGCAGCTGGTGGACGGCGTCATGCGCGCTGCCGCCGAACAGGTCGGCCGGATGCAG
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