TFome Information for pUT6533
| TFome Name | pUT6533 |
| Template | BT069401 |
| Genbank Insert ID | KJ728256 |
| Protein Name | ZmOrphan343 |
| Species | Maize |
| TF Family | Orphans |
| Gene ID | GRMZM2G074824 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information: | Deposited to ABRC |
| 5' Primer Name: | B918_F | 3' Primer Name: | B918_R |
| 5' Primer Tm (º C): | 69.9 | 3' Primer Tm (º C): | |
| 5' Primer Sequence: | caccATGGCTAGTGATGGCCTATGGGA | 3' Primer Sequence: | GTAGATTCGCTCAGCTGTGGAGA |
| PCR Condition: | 10 l GC buffer (Thermo Fisher) 2 l primer forward |
| Nucleotide Sequence: | ATGGCTAGTGATGGCCTATGGGATGTGGTAAGCAACCAGGACGTGCTGTCAATAATCAAGGACACGGTTAAAGAGCCTGGAATGTGCTCGAAGAGGTTGGCCACGGAGGCTGCGGAGCGGGGCAGCAAAGACAATATCACTGTCATTGTTGTCTTTCTTCGCCCCGTCTCCACAGCTGAGCGAATCTAC |
| TFome clones do not contain stop codon at 3'end. | |
| translation: | MASDGLWDVVSNQDVLSIIKDTVKEPGMCSKRLATEAAERGSKDNITVIVVFLRPVSTAERIY |
