| TFome pUT6537 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728260 |
| Protein Name | ZmbZIP14 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM2G153144 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G319A p.A107T Not in functional domain. c.83del147 Exon 3,4 of model skipped. No evidence in RNA-seq for this transcript. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6537
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0061_F |
| Temperature (ºC) | 65.9 |
| Sequence
|
caccATGGGAAGCAATGACCCTAG |
3' Primer
| Name | CD0061_R |
| Temperature (ºC) | |
| Sequence
|
AGACTCGCTCCCAGGCTGGG |
PCR Condition
5 l GC buffer (Thermo Fisher) 1.25 ul DMSO 100% .5
Amino Acid Sequence
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MGSNDPSTPSKASKASKQVLETVDQPPATTSSGTVSVYPEWPSFQTMHPFSHYPMPTNGHAETPGTAPSAPEMNGKSEPGRTSAPSANGITSHSESGSESESEGSDANYENDSHSKDNDGKEDGNSQNSISYSASQGVLNQTMAMLPIQPGAMVGGVSSSTANLNIGVHYWEAPGSAAVSATHGKAPAGSARGDQWDERELKKQKRKQSNRESARRSRLRKQAECEELGQRAETLRSENSSLRAELERIRKEYEQLLSQNASLKEKLGGSSDPIPDMNEQNDGNGSDKKQSDSDAQPGSES
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGAAGCAATGACCCTAGCACACCCTCCAAGGCTTCAAAGGCATCAAAACAAGTACTTGAAACAGTGGATCAACCTCCAGCTACCACATCCAGTGGAACAGTTTCAGTTTACCCTGAATGGCCCAGCTTTCAGACTATGCATCCGTTTAGTCATTACCCTATGCCAACAAATGGACATGCTGAAACTCCTGGAACCGCGCCAAGTGCTCCAGAAATGAATGGGAAAAGTGAGCCTGGCAGAACATCTGCTCCATCTGCCAATGGGATTACCTCGCACAGTGAGAGTGGAAGTGAGAGTGAGAGTGAAGGAAGTGATGCCAATTATGAAAATGATTCACATTCAAAGGACAATGATGGAAAGGAAGATGGCAATTCTCAGAATAGCATATCTTATTCAGCATCACAAGGAGTGTTAAACCAAACCATGGCGATGCTTCCAATACAACCGGGCGCAATGGTTGGAGGAGTTTCTAGCTCCACAGCTAACTTGAATATTGGAGTGCACTACTGGGAAGCTCCAGGTTCTGCAGCTGTCTCTGCAACGCATGGCAAAGCACCAGCTGGTTCAGCTCGGGGAGACCAATGGGATGAAAGGGAACTCAAGAAGCAAAAACGGAAGCAGTCTAATAGAGAATCGGCACGCAGATCCCGGCTGCGCAAGCAGGCTGAGTGTGAGGAGCTTGGGCAACGTGCTGAAACTCTGAGGTCGGAAAACTCGTCTCTCAGGGCCGAGCTTGAACGGATCAGAAAGGAGTACGAACAGCTACTTTCGCAGAACGCTTCACTCAAGGAAAAGCTGGGGGGCAGCAGTGACCCGATTCCTGATATGAATGAACAGAACGACGGCAACGGCAGCGACAAGAAGCAATCTGATTCCGATGCCCAGCCTGGGAGCGAGTCT
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