| PCR Condition:
|
mix per 25l reaction: 5 l GC buffer 5 l 5x CES 1.5 |
| Nucleotide Sequence:
|
ATGAGCAATCAACCACCTCACGCCTCGCTGCAGGATGACCTCCCGGACTGCGACGGCGACGACCCGCTCGCCCTTGCCATCCGGCTCTTCCCCGCCCACACCACCGGCGCGGGTCTATCGCCGGCGGCGCTCGGCATAGGCCGCGTCGCCGAGCCGCCCCGTCGGGAGCAGGAGCCACTGGCTAATAGCACGTATGGCGTACGCGGAGCCGGGCCGGATCCTTGGGGCCTGCGCCTGTCCAGGTCCGTTCTTGGGGGCCTCGACGGATTTGACGTGGACACATTCTTTGCTGACGACGCGCTGGACGGCGGCGGCGAGGCGACCGAAGGCGACTCGCCGCCGGCGCACGTGACAGCCGCCGCGCGCGGCTCGTTGGCCAACGACGACGCGTCGCCGTTGCCCGCGGCCATGCCAGCCGTCGCCCTTGAAGCGTACGACGCGTGCAGTGCACCTGTGCCAGCAGCATGCGGTGGCGCGCCGCCCGTCCTTGTCGCCAACAACAGCATGCCGCCAGCTAGCTGTCTCGGCGCGCGCGGTGCACTTTACATTGTCTCCAAACGCACACGCACGCATGCGCCGCCGCCACTAGCGGCGGAATCCATGACACCGGCAGTCGCTCCTCCTCCAGCCATGCCCGACGGTGGGAACGGTGGCAATAACCGGAGCGCCGCCTTACCTGTCGCTCTCGCTCCTCCGTCCGGGAGTACCGGTGGCGCCGTCCGCCGGCGCCGTCCCGTGCCACGCCCGCGGAACAGGGAAGTCCAGAGGACGTGCAGCCACTGCCAGTCGTCAAAGACGCCGCAGTGGCGCGAGGGCCCGGACGGGCGAAGAACCCTGTGCAACGCGTGCGGGCTACGCTACAAGTCGCATAGGCTGGTGCCTGAGTACCGGGCCGCAGAATCCATGACACCACGCGATCTCCACCCAAACGCTGGGCTGGTCGGCGCGTCTTCAGGTCCGAGCAGCCGCCCGTCGTCGTCATCTACAACGACGCCATCGCAGTCGCAGATCGACTTCCCATCCCACGCCTTGCCGGCAGTCGCTCCTCCTCCAGCCATGCCCGATCCCGACGGTGGGAACGGTGGCAATAACCGGAGCGCCGCCTTGCCTGTCGCTCTCGCTCCTCCGTCGGGGAGTACCGGTGGCGCCGTCCGCCGGCGCCGTCCCGTGCCATGCCCGCGGAACAGGCAAGTTCAGAGGACGTGCAGCCACTGCCAGTCGTCGGAAACGCCGCAGTGGCGCGAGGGCCCGGACGGGCGAAGAACCCTGTGCAACGCGTGCGGGCTACGCTACAGGTCGCATAGGCTGCTGCCTGAGTACCGGCCGACGACGTCTCCTAGCTTCCAGATCGGGCAGCACTCTAATCGCCATCGGAGGATTATGCAGATCAGGGAGCAGAACGGAACAGCTGGGTCA |
|
|
TFome clones do not contain stop codon at 3'end.
|
| translation:
|
MSNQPPHASLQDDLPDCDGDDPLALAIRLFPAHTTGAGLSPAALGIGRVAEPPRREQEPLANSTYGVRGAGPDPWGLRLSRSVLGGLDGFDVDTFFADDALDGGGEATEGDSPPAHVTAAARGSLANDDASPLPAAMPAVALEAYDACSAPVPAACGGAPPVLVANNSMPPASCLGARGALYIVSKRTRTHAPPPLAAESMTPAVAPPPAMPDGGNGGNNRSAALPVALAPPSGSTGGAVRRRRPVPRPRNREVQRTCSHCQSSKTPQWREGPDGRRTLCNACGLRYKSHRLVPEYRAAESMTPRDLHPNAGLVGASSGPSSRPSSSSTTTPSQSQIDFPSHALPAVAPPPAMPDPDGGNGGNNRSAALPVALAPPSGSTGGAVRRRRPVPCPRNRQVQRTCSHCQSSETPQWREGPDGRRTLCNACGLRYRSHRLLPEYRPTTSPSFQIGQHSNRHRRIMQIREQNGTAGS
|
