| TFome pUT6548 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ728271 |
| Protein Name | ZmGATA30 |
| Species | Zea mays |
| TF Family | C2C2-GATA |
| Gene id | GRMZM2G464037 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6548
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | P10up |
| Temperature (ºC) | 63.6 |
| Sequence
|
caccATGAGCAATCAACCACCTCACGC |
3' Primer
| Name | P10dn |
| Temperature (ºC) | |
| Sequence
|
TGACCCAGCTGTTCCGTTCTGC |
PCR Condition
mix per 25l reaction: 5 l GC buffer 5 l 5x CES 1.5
Amino Acid Sequence
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MSNQPPHASLQDDLPDCDGDDPLALAIRLFPAHTTGAGLSPAALGIGRVAEPPRREQEPLANSTYGVRGAGPDPWGLRLSRSVLGGLDGFDVDTFFADDALDGGGEATEGDSPPAHVTAAARGSLANDDASPLPAAMPAVALEAYDACSAPVPAACGGAPPVLVANNSMPPASCLGARGALYIVSKRTRTHAPPPLAAESMTPAVAPPPAMPDGGNGGNNRSAALPVALAPPSGSTGGAVRRRRPVPRPRNREVQRTCSHCQSSKTPQWREGPDGRRTLCNACGLRYKSHRLVPEYRAAESMTPRDLHPNAGLVGASSGPSSRPSSSSTTTPSQSQIDFPSHALPAVAPPPAMPDPDGGNGGNNRSAALPVALAPPSGSTGGAVRRRRPVPCPRNRQVQRTCSHCQSSETPQWREGPDGRRTLCNACGLRYRSHRLLPEYRPTTSPSFQIGQHSNRHRRIMQIREQNGTAGS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAGCAATCAACCACCTCACGCCTCGCTGCAGGATGACCTCCCGGACTGCGACGGCGACGACCCGCTCGCCCTTGCCATCCGGCTCTTCCCCGCCCACACCACCGGCGCGGGTCTATCGCCGGCGGCGCTCGGCATAGGCCGCGTCGCCGAGCCGCCCCGTCGGGAGCAGGAGCCACTGGCTAATAGCACGTATGGCGTACGCGGAGCCGGGCCGGATCCTTGGGGCCTGCGCCTGTCCAGGTCCGTTCTTGGGGGCCTCGACGGATTTGACGTGGACACATTCTTTGCTGACGACGCGCTGGACGGCGGCGGCGAGGCGACCGAAGGCGACTCGCCGCCGGCGCACGTGACAGCCGCCGCGCGCGGCTCGTTGGCCAACGACGACGCGTCGCCGTTGCCCGCGGCCATGCCAGCCGTCGCCCTTGAAGCGTACGACGCGTGCAGTGCACCTGTGCCAGCAGCATGCGGTGGCGCGCCGCCCGTCCTTGTCGCCAACAACAGCATGCCGCCAGCTAGCTGTCTCGGCGCGCGCGGTGCACTTTACATTGTCTCCAAACGCACACGCACGCATGCGCCGCCGCCACTAGCGGCGGAATCCATGACACCGGCAGTCGCTCCTCCTCCAGCCATGCCCGACGGTGGGAACGGTGGCAATAACCGGAGCGCCGCCTTACCTGTCGCTCTCGCTCCTCCGTCCGGGAGTACCGGTGGCGCCGTCCGCCGGCGCCGTCCCGTGCCACGCCCGCGGAACAGGGAAGTCCAGAGGACGTGCAGCCACTGCCAGTCGTCAAAGACGCCGCAGTGGCGCGAGGGCCCGGACGGGCGAAGAACCCTGTGCAACGCGTGCGGGCTACGCTACAAGTCGCATAGGCTGGTGCCTGAGTACCGGGCCGCAGAATCCATGACACCACGCGATCTCCACCCAAACGCTGGGCTGGTCGGCGCGTCTTCAGGTCCGAGCAGCCGCCCGTCGTCGTCATCTACAACGACGCCATCGCAGTCGCAGATCGACTTCCCATCCCACGCCTTGCCGGCAGTCGCTCCTCCTCCAGCCATGCCCGATCCCGACGGTGGGAACGGTGGCAATAACCGGAGCGCCGCCTTGCCTGTCGCTCTCGCTCCTCCGTCGGGGAGTACCGGTGGCGCCGTCCGCCGGCGCCGTCCCGTGCCATGCCCGCGGAACAGGCAAGTTCAGAGGACGTGCAGCCACTGCCAGTCGTCGGAAACGCCGCAGTGGCGCGAGGGCCCGGACGGGCGAAGAACCCTGTGCAACGCGTGCGGGCTACGCTACAGGTCGCATAGGCTGCTGCCTGAGTACCGGCCGACGACGTCTCCTAGCTTCCAGATCGGGCAGCACTCTAATCGCCATCGGAGGATTATGCAGATCAGGGAGCAGAACGGAACAGCTGGGTCA
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