TFome clone pUT6549

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	P17up
Temperature (ºC)	65.0
Sequence Copy to clipboard	caccATGGATACGCTGTTTAGGTCGGTTAGCCTT

3' Primer

Name	P17dn
Temperature (ºC)
Sequence Copy to clipboard	TGGCCTCCACGCGCTTGC

PCR Condition

Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d

Amino Acid Sequence

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MDTLFRSVSLQASEQQQQQQSASYNSRSTTSSGSRSSSHQTDASYNYYYHSNSGVGGVGQYYYGQQNQQYYQEPYQQEECGNAHRLYMDEDFSSSSSSRQHFHSHGAAVQPATSSAVTATAPTPPLSTSSTAAGAAHALFEAADLSFPPDLNLDFSSPASSSGGCVASSAAVGGRWASQLLLECARAVAARDSQRVQQLMWMLNELASPYGDVEQKLASYFLQGLFARLTASGPQTLRTLAAASDRNTSFDSTRRTALRFQELSPWSSFGHVAANGAILESFLEAAAASSEPQRFHILDLSNTFCTQWPTLLEALATRSTDDTPHLSITTVVSAAPSAPTVAVQRRQRVMREIGQRIEKFARLMGVPFSFRAVHHAGDLAELDLDALDLRDGGATTALAINCLNSLRGVVPGGVRRRDAFGASLRRLDPRVVTVVEEEADLVAFDPDASEESGDTEAAFLKVFGEGLRFFSAYMDSLEESFPKTSNERLALERGAGRAIVDLVSCPPSKSMERRETAVSWARRMRSAGFSPVAFSEDVADDVRSLLRRYREGWSMRDTGLDDSAAGAGIFLAWKEQPLVWASAWRP

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGATACGCTGTTTAGGTCGGTTAGCCTTCAAGCCTCCGAGCAGCAGCAGCAGCAGCAGTCGGCGTCCTACAACTCGAGAAGCACCACGTCGAGCGGCTCCAGGTCGTCGTCGCACCAGACCGACGCATCCTACAACTACTACTACCACAGCAACAGCGGCGTCGGCGGCGTCGGGCAGTACTACTACGGTCAGCAGAACCAGCAGTACTACCAGGAGCCATACCAGCAAGAAGAATGCGGCAACGCCCACCGCCTTTACATGGATGAAGACTTCTCCTCCTCGTCTTCGTCGAGGCAGCACTTCCACTCGCACGGCGCGGCGGTGCAGCCGGCGACGTCGTCCGCGGTCACAGCAACGGCGCCGACGCCCCCGCTGTCCACGTCGTCCACGGCCGCAGGAGCGGCACACGCACTGTTCGAGGCGGCCGACCTGTCGTTCCCCCCTGACCTCAACCTCGACTTCTCGTCCCCGGCGTCTTCGTCCGGCGGGTGCGTGGCCTCCTCGGCGGCGGTCGGGGGAAGATGGGCGAGCCAGCTGCTGCTGGAGTGCGCGCGCGCGGTGGCGGCCCGCGACAGCCAGCGCGTGCAGCAGCTGATGTGGATGCTCAACGAGCTGGCCTCGCCGTATGGGGACGTCGAGCAGAAGCTGGCGTCCTACTTCCTCCAGGGGCTCTTCGCGCGCCTCACGGCGTCCGGCCCGCAGACCCTGCGCACGCTCGCGGCGGCGTCCGACCGGAACACGTCCTTCGACTCCACGCGGCGCACGGCGCTGCGGTTCCAGGAGCTCAGCCCGTGGTCGTCGTTCGGGCACGTGGCCGCCAACGGCGCCATCCTGGAGTCGTTCCTGGAGGCCGCCGCGGCGTCGTCGGAGCCGCAGAGGTTCCACATCCTCGACCTGAGCAACACGTTCTGCACGCAGTGGCCCACGCTGCTCGAGGCGCTGGCCACGCGGTCCACCGACGACACGCCGCACTTATCGATCACCACGGTGGTCTCGGCCGCGCCGTCCGCGCCGACGGTCGCCGTGCAGCGCCGCCAGCGCGTGATGCGGGAGATCGGACAGCGGATAGAAAAGTTCGCGCGGCTGATGGGTGTGCCCTTCAGCTTCCGCGCCGTGCACCACGCCGGAGACCTCGCGGAGCTCGACCTCGACGCGCTTGACCTGCGCGATGGCGGCGCCACCACCGCGCTCGCCATCAACTGCCTCAACTCGCTTCGCGGTGTGGTGCCGGGCGGTGTCCGAAGAAGGGACGCGTTCGGCGCGTCGCTCCGGCGTCTCGATCCAAGGGTAGTTACTGTCGTCGAGGAGGAGGCTGATCTCGTGGCTTTTGACCCTGACGCATCCGAGGAAAGCGGCGACACGGAGGCAGCGTTCTTGAAGGTGTTCGGCGAGGGCTTGCGGTTCTTCTCGGCTTACATGGACTCGTTGGAAGAGAGCTTTCCCAAGACTAGCAACGAGAGGCTGGCACTGGAGAGGGGAGCCGGACGTGCCATTGTGGACTTGGTCTCGTGCCCGCCGTCGAAGTCCATGGAACGGCGGGAGACGGCGGTTTCATGGGCACGCCGCATGCGGTCTGCCGGCTTCTCTCCGGTGGCGTTCAGCGAGGACGTTGCCGACGATGTGCGGTCGTTGCTCCGTCGGTATCGGGAAGGCTGGTCGATGCGGGACACCGGTTTAGACGACTCGGCAGCCGGAGCAGGCATCTTCCTGGCGTGGAAGGAACAGCCTCTCGTGTGGGCAAGCGCGTGGAGGCCA