| TFome pUT6564 | |
|---|---|
| Template | BT042239.1 |
| Genbank Insert ID | KJ728285 |
| Protein Name | ZmMYB75 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G070849 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G213T;G694A;G966T; p.V232I;W322C; Not present in template FlcDNA sequence. Not present in functional domains. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6564
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B299_F |
| Temperature (ºC) | 70.7 |
| Sequence
|
caccATGGACATGGCGCACGA |
3' Primer
| Name | B299_R |
| Temperature (ºC) | |
| Sequence
|
CACGCCCTGCGCCTGCGTGAACC |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MDMAHERSDASSEEEVMAGELRRGPWTVEEDLQLVNYVDAHGEGRWNSLARSAGLKRTGKSCRLRWLNYLRPDLRRGNITPQEQLMILELHSRWGNRWSKIAQHLPGRTDNEIKNYWRTRVQKHAKQLKCDVNSQQFKDVMRYLWMPRLVERIQAAAANNNASGAGVPPAGAAADDTPLSSSWQHGGGADDDGGLYASPELPTDACWPPADCYYYPAAADHQLLNNPAAAAIFPDLSSTTAGSSSPSSDSGAGTAQPWPAPLGGAEWLTTGACCDASSAAAALGGQQQQSCLVGETSWASSELPELGVADFEIGSFDVESICSMDDSLWFTQAQGV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACATGGCGCACGAGAGATCAGACGCCAGCAGCGAGGAGGAGGTGATGGCCGGCGAGCTGCGGCGCGGGCCTTGGACGGTGGAGGAGGACCTCCAGCTGGTCAACTACGTCGACGCGCACGGCGAGGGCCGCTGGAACTCGCTCGCCCGATCAGCAGGTCTGAAGCGCACTGGCAAGAGCTGCCGCCTCCGGTGGCTGAACTACCTCCGTCCCGACCTCCGGCGCGGCAACATCACGCCGCAGGAGCAGCTGATGATCCTGGAGCTGCACTCGCGCTGGGGCAACCGCTGGTCCAAGATCGCGCAGCACCTCCCCGGGCGCACCGACAACGAGATCAAGAACTACTGGCGCACGCGCGTGCAGAAGCACGCCAAGCAGCTCAAATGCGACGTCAACAGCCAGCAGTTCAAGGACGTGATGCGCTACCTCTGGATGCCCCGCCTCGTCGAGCGCATCCAGGCCGCCGCAGCCAACAATAACGCCAGCGGCGCCGGGGTTCCGCCAGCTGGCGCCGCCGCGGACGACACGCCGCTCTCGTCGTCGTGGCAGCACGGCGGCGGCGCCGACGACGACGGCGGCCTCTACGCGTCGCCGGAGCTGCCCACGGACGCCTGCTGGCCTCCCGCAGACTGCTACTACTACCCAGCCGCCGCTGACCACCAGCTACTGAACAACCCCGCCGCCGCCGCCATCTTCCCGGACCTGTCAAGCACCACGGCCGGGTCGTCCTCTCCGTCCTCGGATTCCGGCGCGGGGACAGCACAACCCTGGCCCGCGCCGCTGGGTGGCGCAGAGTGGTTGACCACCGGCGCCTGCTGCGACGCCTCCAGCGCCGCCGCCGCCCTGGGCGGGCAGCAGCAACAATCGTGCCTGGTCGGGGAGACGTCGTGGGCGTCATCCGAGCTCCCCGAGCTGGGCGTCGCGGACTTCGAGATCGGCAGCTTCGACGTGGAGAGCATCTGTAGCATGGACGACAGCCTGTGGTTCACGCAGGCGCAGGGCGTG
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