| TFome pUT6565 | |
|---|---|
| Template | BT039909.1 |
| Genbank Insert ID | KJ728286 |
| Protein Name | ZmHB38 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G013617 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G479T p.G160V Not present in template FlcDNA, not located in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6565
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C253_F |
| Temperature (ºC) | 63.3 |
| Sequence
|
caccATGGCCATCCACCACCC |
3' Primer
| Name | C253_R |
| Temperature (ºC) | |
| Sequence
|
CCCAGCGAAATGATGCAGCAA |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MAIHHPHLLDFSPPPNTVAMEEAPPPPHFDHGLLGLHVDGMPHRVLADDAPVAAWAPQAVVSHSLYGYDNTAAGASSLFGHHEASEAQFSVLPPAASSFALLPNHHHQQQLPTTTAASSMQQPFQLRSSKYLGPAQELLAEFCSLEGDLLHATNKQGASVAAAGNSRWDDVETSSSSSAGLWGHLSLSSMDLLELERRKARLLSMVEEVDRRYRRYREQMRSVEVSFEAVAGAGASQVYTRLALRAMSRHFRCLRDALVAQVRALRKAMGERDGGPAGAAAGATKGDTPRLKVLDQCLRQQRAFQHPGTIDNYPWRPQRGLPERAVAVLRAWLFEHFLHPYPNDVDKHILARQTGLSRSQVSNWFINARVRLWKPMIEEMYTEEVNPKPADDTSQNPSAGGGVGVGVAIKPEQQVSTAAAGATIGGGGGDHLFGPSYPSMYGSHGGAVSLTLGLQQQPFASTMMHQRRPLMTFQGDEQEPALPYRDLMGSQLLHHFAG
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCATCCACCACCCGCACCTCCTCGACTTCTCGCCGCCTCCGAACACGGTGGCCATGGAGGAGGCGCCGCCCCCACCCCACTTCGACCACGGCCTCCTCGGGCTCCATGTCGATGGCATGCCTCATCGCGTCCTCGCCGACGACGCGCCGGTGGCGGCATGGGCGCCGCAGGCCGTGGTCTCCCACTCCCTGTACGGATACGATAACACAGCAGCGGGGGCGTCGTCGCTGTTTGGCCACCATGAGGCATCAGAGGCCCAGTTCTCCGTGCTTCCGCCGGCAGCGTCGTCGTTTGCACTACTACCTAACCACCACCACCAGCAGCAGCTGCCGACGACGACGGCGGCGTCGAGCATGCAGCAGCCGTTCCAGCTGAGGAGCTCCAAGTACCTGGGTCCTGCGCAGGAGCTGCTCGCCGAGTTCTGCAGCCTGGAAGGGGACCTGCTGCACGCCACGAACAAGCAGGGAGCTTCCGTAGCAGCAGCAGGTAACAGCAGGTGGGACGACGTGGAGACGTCGTCGTCTTCTTCTGCTGGCCTCTGGGGGCACCTGTCCCTGAGCTCCATGGATCTCCTGGAGCTCGAGAGGAGGAAGGCCAGGCTCTTGTCCATGGTTGAAGAGGTGGACCGGCGGTACCGGCGATACCGCGAGCAGATGAGGTCAGTGGAGGTGTCGTTCGAGGCGGTGGCCGGAGCCGGCGCGTCGCAGGTGTACACGCGGCTGGCGCTGCGGGCCATGTCGCGGCACTTCCGGTGCCTGCGGGATGCGCTGGTGGCGCAGGTGCGCGCTCTGCGGAAGGCCATGGGGGAGAGGGACGGCGGCCCAGCTGGTGCGGCGGCGGGCGCCACCAAGGGCGACACGCCCAGGCTCAAGGTGTTGGACCAGTGCCTGCGGCAGCAGCGGGCGTTCCAGCACCCGGGCACCATCGACAACTACCCGTGGCGGCCCCAGCGCGGCCTGCCGGAGCGCGCCGTCGCCGTCCTCCGAGCCTGGCTCTTCGAACACTTCCTCCACCCGTATCCGAACGATGTGGACAAGCACATTCTAGCGCGCCAGACAGGACTGTCAAGAAGCCAGGTTTCCAATTGGTTCATCAACGCCAGGGTGAGGCTGTGGAAGCCGATGATAGAGGAGATGTACACGGAAGAAGTGAACCCGAAACCGGCCGACGACACAAGCCAAAACCCTAGCGCCGGTGGCGGCGTCGGCGTCGGCGTCGCCATCAAACCTGAGCAGCAGGTGAGCACAGCTGCGGCGGGAGCCACCATCGGAGGCGGCGGCGGCGACCATCTGTTCGGTCCTAGTTATCCCAGCATGTACGGGAGCCACGGCGGCGCCGTGTCGCTTACCCTAGGGCTCCAGCAGCAGCCGTTTGCGTCGACGATGATGCACCAGCGACGACCACTGATGACGTTTCAAGGTGACGAGCAAGAGCCGGCGCTGCCGTACAGAGACCTTATGGGCTCTCAGTTGCTGCATCATTTCGCTGGG
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