| TFome pUT6568 | |
|---|---|
| Template | BT041934 |
| Genbank Insert ID | KJ728289 |
| Protein Name | ZmOrphan51 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G133555 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6568
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B951_F |
| Temperature (ºC) | 66.4 |
| Sequence
|
caccATGATTAGAATTTTTATCCTGGAGGATGG |
3' Primer
| Name | B951_R |
| Temperature (ºC) | |
| Sequence
|
CAGCCAGGATTCGACGGTG |
PCR Condition
5 l GC buffer (Thermo Fisher) 1 l primer forward 2
Amino Acid Sequence
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MIRIFILEDGISSPIAAHIFDFCDNGSGGDLFTAVNVASDLFTAPSEDASSSSVVTTPPAPCGHGDDMPLGTAAAPAASAFSPMPSFDSTLSALLEEEDPSVPNPELLLPIDYPFAAAAAGDEPHPEQQQQQFAQVPVMLPLAGAAQHPGLQTHSTASELMQLDECFAAAMAGGGGYVGLNESMCQQPQPGALFSTTGGMMETGAPGCYFDKDMAAAAAAQGGFLGTGCNGMMMSIMGMEEIGEYQRMMEGASDSLAGTHIPDADCAAAAQMAFSGNAVAGEMQMGGSMSPGRLPAATATEASSLEDANFKSARITVEERREKIIRYIKKRNKRNFRKKIKYACRKTLADSRPRVRGRFAKNDDYGEPSSRVMQNHEEYDQMAASMKAEDILDPDAAHFNGMNSYLFNHTVESWL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATTAGAATTTTTATCCTGGAGGATGGCATCTCGAGCCCCATAGCAGCGCACATCTTTGACTTCTGCGACAATGGCAGCGGCGGCGACCTCTTCACGGCCGTGAACGTGGCCTCAGACCTGTTCACGGCGCCATCAGAGGATGCCTCGTCGTCGTCCGTAGTGACGACGCCTCCCGCGCCCTGCGGCCACGGCGACGACATGCCGTTGGGCACCGCCGCGGCGCCTGCTGCCTCGGCCTTCTCCCCCATGCCGTCCTTCGACTCCACCCTCTCCGCTCTCCTCGAAGAAGAAGACCCATCGGTTCCAAACCCAGAACTCCTCCTACCCATCGACTACCCGTTCGCGGCGGCGGCGGCTGGAGACGAGCCTCACCCCGAACAGCAGCAGCAGCAGTTCGCGCAGGTGCCAGTAATGCTTCCACTGGCCGGTGCGGCCCAGCACCCGGGTCTGCAGACGCACAGCACGGCGTCCGAGCTGATGCAGCTAGACGAGTGCTTCGCGGCGGCAATGGCTGGAGGAGGAGGGTACGTGGGCCTAAACGAGTCCATGTGCCAGCAGCCGCAGCCCGGCGCGTTGTTCTCCACCACCGGCGGCATGATGGAAACCGGCGCGCCGGGCTGCTACTTTGACAAGGACATGGCGGCGGCGGCGGCGGCGCAGGGTGGCTTCTTGGGGACCGGTTGCAATGGCATGATGATGTCGATAATGGGGATGGAGGAGATCGGCGAGTACCAGCGGATGATGGAGGGCGCCAGCGACTCGCTGGCCGGCACGCACATTCCCGACGCGGACTGTGCCGCCGCGGCACAGATGGCGTTCAGCGGCAACGCCGTTGCCGGGGAGATGCAGATGGGCGGCAGCATGAGCCCGGGGCGGCTGCCGGCGGCGACGGCGACGGAGGCGTCGAGCCTGGAGGACGCCAACTTCAAGAGCGCCCGCATCACCGTGGAGGAGAGGCGGGAGAAGATCATCAGGTACATCAAGAAGCGGAACAAACGCAACTTCAGAAAGAAGATCAAGTACGCTTGCAGAAAGACCCTGGCGGACAGCAGGCCCCGCGTCCGCGGCAGGTTCGCCAAGAATGACGACTACGGCGAGCCGTCGTCAAGGGTGATGCAGAACCACGAGGAGTACGACCAAATGGCGGCCAGCATGAAGGCAGAAGACATTCTCGATCCCGACGCCGCGCACTTCAACGGGATGAATTCCTACTTGTTCAACCACACCGTCGAATCCTGGCTG
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