TFome clone pUT6594

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	CD0138_F
Temperature (ºC)	73.2
Sequence Copy to clipboard	caccATGGGGCGATcaccATGCTGTGAGA

3' Primer

Name	CD0138_R
Temperature (ºC)
Sequence Copy to clipboard	GACCAAGGAAGGCGACTGTGCTGTC

PCR Condition

PCR Conditions: Volumes per reaction (30l): 6 l GC

Amino Acid Sequence

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MGRSPCCEKIGLKKGPWTPEEDEKLLAFVEEHGHGSWRALPAKAGLQRCGKSCRLRWTNYLRPDIKRGKFSLQEEQTIIQLHALLGNRWSAIATHLPNRTDNEIKNHWNTHLKKRLAKIGIDPVTHKSTCGTLTGTTNDRSAKAAASLSHMAQWENARLEAEARLARESKTRTATPTPSALHAQPMDLPASAASPWLDVLHAWQGAKIDLESPTSTLTFTGSNGGMLPTPRTNGPEVSESNSAMSHYQMSDELEGEETYWQIFSKHQVPEVDSKESEDDFIGCEEPWFSGMAGVGAGMLLDVSNEHELSECWGESSSGQTVEHSKQASDKEDKDYWNWVLDRVNSELTAQSPSLV

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGGCGATCACCATGCTGTGAGAAGATTGGACTGAAGAAAGGTCCATGGACGCCGGAGGAGGATGAGAAGCTGCTTGCCTTCGTTGAGGAACATGGACACGGGAGCTGGCGGGCATTACCTGCGAAGGCAGGCTTGCAGAGGTGTGGGAAGAGCTGCAGGTTGAGGTGGACAAACTACCTGAGGCCGGACATCAAGAGGGGCAAGTTCAGCTTGCAAGAAGAACAGACCATCATCCAGCTTCACGCTCTTTTAGGCAACAGGTGGTCGGCCATTGCAACACATCTACCAAATCGCACGGACAACGAGATCAAGAACCACTGGAACACGCACCTCAAGAAAAGGCTGGCCAAGATTGGGATCGATCCTGTCACCCACAAATCTACCTGTGGCACTCTCACTGGCACCACGAACGACAGATCAGCCAAGGCCGCGGCAAGCCTCAGCCACATGGCACAATGGGAGAATGCCCGCCTCGAGGCTGAGGCACGGCTGGCTCGAGAATCGAAGACACGAACAGCAACACCGACGCCATCTGCACTCCATGCGCAGCCAATGGATCTACCTGCCTCTGCTGCTTCTCCATGGCTTGACGTGTTGCATGCTTGGCAGGGTGCAAAGATAGACCTGGAGTCACCTACCTCCACACTGACGTTTACAGGGAGCAATGGTGGCATGCTGCCAACCCCCAGGACCAACGGACCAGAGGTATCAGAAAGCAACTCCGCGATGTCGCATTATCAGATGAGCGATGAGTTGGAGGGTGAAGAAACCTATTGGCAGATCTTCAGCAAGCACCAAGTGCCGGAAGTGGACAGCAAGGAGAGTGAAGATGACTTCATTGGCTGTGAGGAGCCGTGGTTCTCAGGGATGGCTGGGGTTGGAGCTGGCATGCTGCTTGATGTATCCAATGAGCATGAGCTATCAGAATGCTGGGGTGAGTCCAGCAGTGGCCAAACTGTTGAGCACAGCAAGCAAGCATCCGATAAGGAGGACAAGGATTATTGGAATTGGGTCCTTGACAGAGTAAACTCAGAGCTGACAGCACAGTCGCCTTCCTTGGTC