TFome clone pUT6602

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	CD0334_F
Temperature (ºC)	66.6
Sequence Copy to clipboard	caccATGGGTAAGAAGGGAAATTGGTTC

3' Primer

Name	CD0334_R
Temperature (ºC)
Sequence Copy to clipboard	CTTAAAGGACCTCGTGCCGAG

PCR Condition

PCR reaction: 25 uL vol 5xGC buffer 5 uL 10 mM dNT

Amino Acid Sequence

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MGKKGNWFTALKKAFTSSPKEKPTNVHQLVAQYPSTHAHGWEKKRWGGFGRSRPHAEPASPAAGALINIPLYREPSSIEKILGDAEMDQQRQYYAATRAQYQITPARPTTAVAGSAASAAAVPLPQPVVGVGTARERERRREDKPAAVVLPLPPPSPPPLIRRFDHDREQQKKLQLQPQSCRAETEWRRQQQQPRRHRAARQSAAPPDRARAAAVAIQAAFRGYMARRNYRSLRGLIRLQGVMRGASVRRQTAQAMRCMQTLVRVQAQVRASRVEAMERRNRQHHGAMLRDGGRWRASSQDGGFWDASRLTREEADARTKRKVEAVIKRERALAYAYSHQLLKATPMAAHAILADLQSGRSPWWWTPIERRHEPGAYRPADPAIRKPLPALAIAHRDMTTTPVAMTAATTPARSVVSAYSKTRATRPVIRVGAPPPSNHSHVGSIRDDESLTSCPAFGGVPNYMTPTMSASAKARARAHLQEQQKNKAAQEKPRFSFGLGQSIGSWAKSPFWKAAVPSSRVATPAASMAGGRHRSTRSISGLSTDSTVSMPARLGTRSFK

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGTAAGAAGGGAAATTGGTTCACTGCGCTCAAGAAGGCCTTCACCTCAAGCCCCAAGGAGAAGCCAACAAATGTGCATCAGCTGGTAGCGCAGTACCCGTCTACTCACGCGCACGGCTGGGAGAAGAAGCGATGGGGAGGCTTCGGGCGGTCGCGGCCGCACGCGGAGCCGGCGTCCCCGGCGGCGGGCGCGCTGATCAACATCCCGCTGTACCGCGAGCCGAGCAGCATCGAGAAGATCCTCGGCGACGCGGAGATGGACCAGCAGCGCCAGTACTACGCCGCCACCAGGGCGCAGTACCAGATCACGCCCGCGAGGCCCACCACCGCCGTCGCCGGTAGTGCTGCTTCTGCTGCTGCCGTGCCGCTGCCGCAGCCGGTGGTGGGAGTGGGAACAGCACGTGAGCGCGAGCGCAGGAGAGAAGACAAGCCGGCCGCCGTGGTGCTCCCGCTGCCGCCGCCGTCCCCTCCCCCGCTGATCAGGAGGTTCGACCATGACAGGGAGCAGCAGAAGAAGCTGCAGCTGCAGCCGCAGAGCTGCAGGGCCGAGACGGAGTGGCGGCGGCAGCAGCAGCAGCCGAGGCGGCACCGCGCGGCGAGGCAGAGCGCCGCGCCGCCGGACCGCGCGCGCGCCGCGGCCGTGGCGATCCAGGCCGCATTCCGGGGCTACATGGCGCGGCGGAACTACCGGTCGCTGCGCGGGCTGATCCGGCTGCAGGGCGTGATGCGGGGCGCCAGCGTGCGGCGGCAGACGGCGCAGGCGATGCGGTGCATGCAGACGCTGGTCCGCGTGCAGGCGCAAGTGCGCGCCAGCCGCGTGGAGGCCATGGAGCGGCGCAACCGGCAGCACCACGGCGCCATGCTCAGGGACGGCGGCAGGTGGCGCGCCAGCTCTCAGGACGGCGGATTTTGGGACGCCAGCCGGCTGACGCGGGAGGAGGCAGACGCGCGGACGAAGCGGAAGGTGGAGGCCGTGATCAAACGCGAGAGAGCCCTCGCCTACGCCTACTCCCATCAGCTGCTGAAGGCGACACCGATGGCGGCCCACGCGATCCTGGCGGACCTGCAGTCGGGGCGGAGCCCCTGGTGGTGGACGCCCATCGAGCGCCGCCACGAGCCGGGGGCCTACCGCCCTGCGGATCCCGCCATTAGAAAGCCCCTGCCGGCGCTCGCCATCGCCCACCGCGACATGACGACGACGCCTGTGGCGATGACGGCGGCGACGACACCGGCCCGGTCGGTGGTCTCGGCCTACTCTAAGACAAGGGCCACGAGGCCTGTGATCAGGGTGGGAGCCCCGCCGCCTTCGAACCATAGCCACGTGGGGAGCATCCGCGACGACGAGAGCCTGACGAGCTGCCCGGCATTCGGCGGCGTGCCCAACTACATGACGCCTACGATGTCGGCGTCCGCCAAGGCACGGGCACGGGCGCACCTGCAGGAGCAGCAGAAGAACAAGGCGGCCCAGGAGAAGCCCAGGTTCTCGTTCGGGCTGGGCCAGAGCATCGGGAGCTGGGCCAAGAGCCCGTTCTGGAAGGCCGCTGTGCCGTCGTCGAGGGTGGCCACGCCGGCGGCGTCGATGGCCGGCGGGCGACACCGATCGACGCGGTCGATCAGCGGGCTTAGCACGGACTCCACCGTGTCCATGCCAGCCAGGCTCGGCACGAGGTCCTTTAAG