| TFome pUT6606 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728325 |
| Protein Name | ZmWRKY91 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G441031 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6606
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0189_F |
| Temperature (ºC) | 66.3 |
| Sequence
|
caccATGAACATCCTTGAATCTTCCACTC |
3' Primer
| Name | CD0189_R |
| Temperature (ºC) | |
| Sequence
|
CGGTGCAATCAATGGCTGTCTACAT |
PCR Condition
PCR Conditions: Volumes per reaction (30l): 6 l GC
Amino Acid Sequence
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MNILESSTRSGCQVVLNEIEHQGALMRDLYDLILPTLDPCSRQEKLVQQIFQDISSSSGKVLSFLELGDNSKKQANLIKHRRKRGKNSGVESHMLGEEAKEIGNKRRKNAQHTGSIVTQAPHFDGYKWRKYGQKWISKAKHSRSYYRCANSKDQGCLATKTVQQKESDGSGTVSLFDVEYYGHHICKKDVVNHPCVVDTAHYYSVPIANQNQSSSSPTFVHNDVYGIQDESFENLFMVPSIPEYLTDFTDIEMAGALEVTSEMISENIWAYEMISENICRQPLIAP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAACATCCTTGAATCTTCCACTCGTAGTGGCTGCCAAGTGGTGCTCAACGAGATTGAACACCAAGGGGCTCTCATGAGGGACCTATATGACCTTATCCTACCAACACTTGATCCCTGTAGTAGGCAGGAGAAGCTTGTTCAGCAAATCTTTCAAGATATATCCAGTTCCTCAGGTAAGGTCCTCTCCTTTCTCGAACTTGGTGATAACAGTAAGAAGCAGGCCAATCTTATCAAACATAGAAGAAAACGTGGTAAGAATAGCGGCGTGGAGAGCCACATGCTGGGGGAGGAAGCTAAAGAAATTGGAAATAAAAGAAGGAAGAATGCACAACACACAGGTTCAATTGTGACACAAGCACCGCACTTCGATGGATATAAATGGAGGAAGTATGGGCAGAAGTGGATCTCCAAAGCAAAGCATTCTAGGAGCTACTATAGATGTGCCAATAGTAAAGACCAAGGGTGCCTTGCAACCAAGACAGTTCAACAGAAGGAATCAGATGGAAGCGGGACAGTGAGCTTGTTCGACGTTGAATATTACGGCCACCACATTTGCAAGAAGGATGTCGTCAACCATCCGTGTGTTGTTGACACAGCACACTATTACAGTGTGCCGATTGCCAATCAGAACCAAAGCAGTAGCAGCCCAACGTTTGTTCACAATGATGTCTATGGAATCCAGGATGAAAGCTTTGAAAACCTGTTCATGGTGCCGAGCATACCAGAGTATCTGACAGATTTCACAGACATTGAAATGGCGGGGGCACTTGAGGTTACCTCCGAGATGATCTCTGAAAATATATGGGCGTACGAGATGATCTCTGAAAATATATGTAGACAGCCATTGATTGCACCG
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