| TFome pUT6610 | |
|---|---|
| Template | BT063032 |
| Genbank Insert ID | KJ728329 |
| Protein Name | ZmOrphan80 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM5G812926 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C12T p.(=) Not present in FLcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT6610
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C497_F |
| Temperature (ºC) | 68.2 |
| Sequence
|
caccATGGCGTCCGTCAAGCT |
3' Primer
| Name | C497_R |
| Temperature (ºC) | |
| Sequence
|
GGCATCCGCGGCCAGCT |
PCR Condition
PCR Conditions: Volumes per reaction (25l): 5 l GC
Amino Acid Sequence
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MASVKLGSKPDAFKRQGQAWFCTTGLPSDVTVEVGDMSFHLHKFPLLSKSAFLERSIEENSDQEECIIKLNDIPGGAKSFELVARFCYGVKIELSPANVVYLRCASEHLEMTEEVAEENLIAQSEMFLNQVVLRNWKDSLAALETCDDLLPHAEDLQIVNRCIESLASKATSNPNLFGWPIREHGMMQSPGGSVLWNGISTGARPRNFNSDWWYGDASSLSFPMYKRLISTMESRGIRPEIIAGSLTYYAKKYLPGLRRHSHSMGTMPLTATLSEVEQKKLLEEIDRLLPLHRGIASTKVLLGLLRTAMILKASPTCISNLEKRIGMQLDQATLEDLLLPNFSYTMETLYNVECVHRILDHFLAMDQANGGESPCLDDVMASPSLTPITSVAKLIDGYLAEIAPDINLKPPKFQALASAIPEYARPLDDGLYRAIDIYLKAHSWLSEAEREQFCRLLDCQKLSLEACTHAAQNERLPLRVVVQVLFFEQLQLRTSIAGCLLVSDNLEGSRPLRSGIATSGEAGGWATAVRENQVLKVGMDNMRTRLAELEKECSSMRQEIEKLGGRAGKGGGGGWASRVVPRRLGLKVRSQMCSAQEGSVSEQHRSMSAKLDKLQAKVSKQKKQLAADA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGTCCGTTAAGCTCGGCTCCAAGCCTGACGCCTTCAAGCGGCAAGGCCAGGCATGGTTCTGCACAACAGGACTTCCTAGCGATGTCACCGTTGAGGTTGGGGATATGTCTTTCCACCTTCACAAGTTCCCTTTACTTTCAAAAAGCGCCTTTCTTGAACGGTCAATTGAAGAAAACTCTGACCAAGAAGAATGCATCATCAAACTAAATGACATTCCTGGGGGTGCCAAGTCATTTGAGCTAGTTGCAAGGTTCTGCTATGGTGTGAAAATAGAGCTTTCACCTGCAAATGTTGTCTACCTACGGTGTGCCTCAGAGCATCTAGAAATGACTGAAGAAGTAGCTGAGGAAAACCTGATTGCACAGTCAGAAATGTTCCTCAACCAAGTGGTTCTCCGTAACTGGAAAGATTCCTTGGCAGCATTGGAAACATGTGATGACCTACTCCCCCATGCTGAAGACCTCCAGATTGTGAACAGATGCATCGAGTCATTGGCATCAAAAGCTACTAGTAATCCAAACCTCTTCGGATGGCCGATAAGGGAACATGGCATGATGCAAAGTCCTGGTGGCAGTGTACTATGGAATGGGATCAGCACGGGTGCAAGGCCCAGAAATTTCAATTCAGACTGGTGGTATGGTGATGCTTCATCATTGAGTTTTCCCATGTATAAGAGGTTAATCTCCACCATGGAGTCTCGAGGCATCCGGCCTGAAATAATTGCTGGTTCCTTAACCTACTATGCCAAGAAGTATCTTCCAGGACTTAGGCGTCATAGTCATAGCATGGGCACAATGCCTCTTACTGCTACTCTATCTGAGGTTGAACAAAAAAAATTACTTGAGGAGATCGACAGGCTTTTGCCTCTTCACAGGGGTATAGCATCGACAAAAGTTCTGCTTGGGCTCCTTCGCACTGCCATGATTCTGAAAGCCAGCCCCACGTGCATTTCTAACTTGGAGAAACGAATTGGCATGCAACTAGACCAGGCCACTTTGGAGGATCTGCTGCTGCCAAATTTCTCCTACACAATGGAAACACTGTACAATGTGGAGTGTGTGCACAGGATTCTTGATCATTTCTTGGCAATGGACCAAGCAAATGGTGGCGAGTCCCCATGCTTGGATGATGTCATGGCATCCCCATCCTTGACACCGATCACAAGTGTTGCCAAGCTAATTGACGGCTATCTTGCAGAAATTGCACCAGATATCAATTTGAAGCCTCCAAAGTTCCAAGCTCTGGCCTCTGCTATTCCTGAATATGCCCGGCCACTGGATGATGGCCTTTATCGTGCCATTGATATATACTTGAAGGCTCATTCCTGGCTGTCAGAAGCTGAAAGGGAGCAGTTCTGCCGGCTGTTGGACTGCCAGAAGCTCTCCCTGGAAGCATGCACCCACGCCGCCCAGAACGAGAGGCTGCCGTTGCGCGTGGTGGTGCAAGTCCTCTTCTTCGAGCAACTCCAGCTAAGAACCTCGATCGCAGGGTGCCTGCTGGTCTCGGACAACCTCGAAGGCTCCAGGCCTCTGCGGAGCGGCATTGCCACATCCGGCGAGGCTGGAGGCTGGGCCACGGCTGTCCGAGAGAACCAGGTCCTGAAGGTCGGCATGGACAACATGCGGACGCGGCTGGCCGAGCTGGAGAAGGAGTGCTCGAGCATGCGGCAGGAGATCGAGAAGCTGGGCGGGCGCGCTGGTAAGGGCGGCGGTGGCGGGTGGGCCTCCCGCGTCGTGCCCCGGCGACTGGGCCTGAAGGTGAGGTCGCAGATGTGCAGCGCCCAGGAGGGCTCCGTCAGTGAGCAGCACAGGAGCATGAGCGCCAAGCTCGACAAGCTGCAGGCTAAGGTGTCGAAGCAGAAGAAGCAGCTGGCCGCGGATGCC
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