| TFome pUT6632 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728350 |
| Protein Name | ZmOrphan192 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G416184 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C660T p.(=) |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6632
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0399_F |
| Temperature (ºC) | 68.2 |
| Sequence
|
caccATGAAGTTCATGAAGCTGGGCTC |
3' Primer
| Name | CD0399_R |
| Temperature (ºC) | |
| Sequence
|
AGAAACAGAGTACCTCCTGCCC |
PCR Condition
5xGC buffer 5 uL 10 mM dNTP 1 uL 100% DMSO 1.25 uL
Amino Acid Sequence
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MKFMKLGSKPDAFQSDGADVRYVVSDLATDVVVHVGEVKFYLHKFPLLSKSGKLQRLVLKAAEDETDEVRIDDLPGGAKSFEICAKFCYGMVVTLSPHNVVAARCASQYLEMTEDMEKGNLIFKIEVFINSSILRSWKDSIIVLQSTKALSPWSEELKVVGRCIDAIASKTSADPANVTWSYSHSSKKGGACAEIVEPAGKPSIAPKDWWVEDLCELDVDLYKRVMVAIKSKGRMSPELIGEALKAYAVRWLPDSYDALVAEDYMRRNQCLVETIIWLLPSDKSSGCSCRFLLKLLKVAILVGGGEHVKEELIRRISFQLHKATVKDLLLPATSPNDGTYDVRLVHNLVQRFVARTALSHNGGFIEKSDEKMIELNFEQESILSVGELVDGYLSEVASDQDLSLSMFVELATAVPEVARPVHDNLYYAVDAYLKEHPDINKSEKKKICSLVDVKKLSADASVHATQNDRLPLRLVVQVLFFQQLRAGPSKELALIDNGEHACTRPTQDQCDRGERRIPKHPNSLNKQVTSLSARESQQHRISEHRGGRNSFKDQLGGLLLQSRSRRIFDKLWSSKGQGEHGGKGSETSGSSQSPPLSAKPAEVKPSPLPPLRGRRYSVS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAAGTTCATGAAGCTGGGCTCCAAGCCCGACGCCTTCCAGTCCGACGGCGCCGACGTCCGATATGTGGTCTCTGACCTTGCAACAGATGTTGTGGTGCATGTTGGTGAAGTAAAATTTTACTTGCACAAGTTTCCCCTCCTTTCCAAGAGCGGCAAGCTTCAAAGATTAGTTCTGAAGGCCGCTGAGGACGAGACTGATGAGGTTCGCATAGATGACCTTCCTGGGGGAGCAAAATCATTCGAAATATGTGCCAAGTTCTGCTATGGAATGGTGGTCACCCTCAGTCCACACAACGTTGTAGCTGCAAGATGTGCGTCACAGTATCTTGAAATGACCGAAGATATGGAAAAGGGGAACTTGATATTTAAAATCGAGGTCTTCATAAATTCTAGCATACTTCGTAGCTGGAAGGACTCTATCATTGTTCTCCAGAGCACAAAGGCTCTATCACCATGGTCTGAAGAGCTGAAGGTTGTGGGTAGATGTATCGATGCCATCGCTTCGAAGACGTCTGCGGATCCTGCCAATGTAACCTGGTCATACAGTCATAGTAGCAAGAAAGGAGGAGCTTGTGCTGAAATAGTCGAGCCAGCAGGAAAGCCATCAATAGCTCCAAAAGATTGGTGGGTTGAGGACTTGTGTGAACTGGATGTTGATCTGTACAAGAGAGTCATGGTTGCTATCAAATCCAAGGGGAGAATGTCACCTGAACTGATAGGAGAGGCACTTAAAGCCTATGCAGTTAGATGGCTGCCAGACTCCTACGATGCATTAGTTGCCGAGGATTACATGAGGAGGAATCAATGTCTGGTTGAGACAATAATATGGTTGCTGCCTTCTGACAAGAGCTCGGGATGTTCTTGCAGATTTCTCCTGAAACTGTTGAAAGTGGCCATACTAGTCGGAGGCGGGGAGCACGTGAAGGAAGAGCTTATCAGGAGGATCAGCTTCCAGTTGCACAAAGCTACTGTTAAGGATCTCTTATTACCTGCCACTTCGCCTAATGATGGTACCTATGATGTTCGGCTGGTGCATAATCTTGTCCAGAGATTTGTTGCAAGAACAGCGCTGTCTCATAATGGCGGTTTCATTGAGAAGTCCGACGAAAAGATGATTGAGCTCAACTTCGAGCAAGAATCCATTTTGTCGGTAGGAGAGCTTGTTGATGGCTACCTGTCTGAAGTAGCTTCGGATCAAGACCTTTCGTTGTCTATGTTTGTTGAACTGGCGACTGCCGTGCCAGAGGTGGCTAGGCCTGTTCATGACAACCTGTACTATGCAGTCGATGCATATCTCAAGGAACACCCAGACATAAACAAGTCCGAGAAGAAGAAGATTTGCAGTCTGGTGGATGTCAAGAAGCTCAGCGCAGATGCATCCGTGCACGCCACACAAAACGACCGCCTGCCTCTCCGGTTGGTGGTTCAGGTCCTCTTCTTCCAGCAGCTGAGGGCCGGCCCAAGCAAAGAACTCGCCCTGATTGACAATGGAGAACACGCTTGCACACGGCCTACGCAGGATCAATGTGACCGTGGTGAAAGGCGGATACCGAAGCATCCAAACTCACTGAATAAGCAGGTGACAAGCCTGAGCGCGAGAGAGAGCCAGCAGCACCGGATCAGCGAGCACAGGGGCGGCAGGAACAGCTTTAAAGACCAGCTTGGGGGCCTCCTGCTGCAGTCGCGATCCAGGAGGATCTTTGACAAGCTGTGGAGCAGCAAAGGGCAGGGCGAGCATGGCGGGAAAGGCTCGGAGACGTCTGGTAGCTCGCAGAGCCCACCGTTGTCAGCAAAGCCTGCAGAAGTGAAGCCCTCCCCTCTTCCCCCTCTTAGGGGCAGGAGGTACTCTGTTTCT
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