TFome clone pUT6633

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	CD0377_F
Temperature (ºC)	69.1
Sequence Copy to clipboard	caccATGGCGCGCTCCAAGAAC

3' Primer

Name	CD0377_R
Temperature (ºC)
Sequence Copy to clipboard	GATATGCCACTTCCTGTCAGACG

PCR Condition

5xGC buffer 5 uL 10 mM dNTP 1 uL 100% DMSO 1.25 uL

Amino Acid Sequence

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MARSKNGCLKILVCAGSSSDPSAGSDADAADHPDENKAISDKSIWSFRRRSTRHRVLKNSDISEPETVSSSKAKADIAPNNNVYTSTYSFASEKPLQHNKPNENVLHQEKPEEMPLHQDSSEKLMEKPIEKHIDKLMEEPADQIIEKSIELPTQKITETPTDEPAEKINETPTGEPAEKIIEAASENTEGTIKNATEETHEMAVEELIEEPEETISVSSTGLKQGENTSLVEGSSADDEEDHLECAATNLQPGNDTCLEREEVLNQKDLVKLQAVIRGHLVRKQALESLQCLLAIIKIQGLIRAHQAQHSPGMIQETIVHSSGEKLLRNGFALKLMDNMSTSKSICIKCDPSESDVTWEWMERWTTLIPPITVVHVPEHKENSELMGETVTEQVTEHAQCDEDIVALDSDICFPKLVADDVKETVETSESSTLEAPVSVPDETPKMEMKHDTESELIETTNVDAEQVTDQKAENDVDKAFMSLNQQYTQADASRDPCPLSEKFESSKEDSGDAYNSEQTLEMEGKRFVVRKSCNPAFAAAQLKFEELSTNSTVSRSSSLSHLDGASKSRVHAPSSQEDCSSKQDGTGMPDRSVGHDTKMIIAASECGTEISISSTLDSPDRSEGDGGEIVLEIGALENRNYVTSKADNDFNTVHPEVKNAPEVEAQPQKQIEQNGHVPALEIEAQPQDELVQEPHAEPEMPGLHERLEKPVASYATPEGTPMSRTTITESQETPSSEVSVNTKKSRSKKPKPHASKRSLASPGSDSVGRSSTDNFSKESRHPKRESSSKAAKSDHVEQEPRTSNSNSNLLPSYMQFTESARAKASSSPKMSPDVQDSNPRKRHSLPMTDGKHDSSPRMQRSSSHAQQNVKSNGPAPHSASDRKWHI

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGCGCTCCAAGAACGGCTGCCTCAAGATCCTCGTCTGCGCCGGCTCCAGCTCCGATCCCTCCGCCGGCTCCGACGCCGACGCCGCCGACCACCCCGACGAGAACAAGGCCATATCAGATAAAAGCATATGGAGCTTTCGTAGGAGATCTACAAGGCATCGAGTTTTGAAGAACTCTGATATCTCAGAACCTGAAACTGTTAGTTCCAGCAAAGCGAAGGCTGACATTGCACCAAACAACAATGTTTATACTTCAACATATTCTTTTGCCTCGGAGAAGCCTCTGCAACATAACAAGCCAAATGAAAATGTTCTGCATCAAGAGAAACCAGAGGAAATGCCTTTGCATCAAGATAGCTCCGAAAAGCTGATGGAGAAGCCAATTGAAAAGCATATTGACAAACTTATGGAAGAACCTGCTGACCAGATAATTGAAAAATCAATTGAACTACCTACTCAAAAGATAACTGAAACACCAACTGATGAGCCAGCTGAAAAAATAAATGAAACACCAACTGGAGAACCAGCTGAAAAAATAATAGAGGCGGCATCTGAAAATACAGAAGGGACAATAAAAAATGCAACCGAAGAGACACATGAAATGGCAGTTGAGGAACTAATTGAAGAGCCAGAAGAAACTATTTCTGTTTCATCAACTGGGCTAAAGCAGGGGGAAAACACCTCACTTGTTGAAGGAAGCAGTGCAGACGATGAGGAAGATCATTTGGAGTGTGCAGCTACTAATCTTCAGCCTGGCAATGACACTTGCCTTGAAAGGGAAGAGGTACTGAACCAGAAGGATCTGGTGAAACTGCAAGCTGTTATACGTGGACATCTAGTTAGAAAGCAGGCTTTAGAATCTTTACAATGTTTGCTTGCGATTATTAAAATTCAAGGGTTAATCAGGGCTCATCAAGCACAACATTCACCAGGAATGATTCAGGAGACTATTGTTCATTCTTCAGGTGAGAAATTGCTTCGCAATGGATTTGCTCTCAAGCTCATGGACAACATGTCGACGTCAAAATCCATTTGCATAAAATGTGATCCTTCAGAATCTGATGTTACATGGGAATGGATGGAGAGGTGGACAACCTTAATTCCACCAATCACTGTGGTGCACGTGCCAGAGCATAAAGAAAATAGTGAGTTGATGGGTGAAACTGTGACCGAACAAGTGACTGAACATGCTCAGTGTGATGAAGACATTGTTGCTTTGGACTCAGACATATGTTTCCCCAAGTTGGTGGCTGATGACGTGAAAGAAACAGTAGAGACATCTGAATCTAGTACCTTGGAGGCTCCAGTGAGTGTTCCAGATGAAACCCCAAAGATGGAAATGAAACATGATACTGAATCAGAGTTGATCGAAACTACTAATGTTGACGCTGAGCAAGTAACTGACCAAAAGGCTGAAAATGATGTGGACAAGGCTTTTATGTCCTTGAATCAACAATATACCCAAGCAGATGCATCGAGGGATCCCTGTCCACTTTCTGAAAAATTTGAATCATCCAAGGAGGACAGTGGCGATGCGTACAATTCTGAGCAGACACTGGAGATGGAAGGTAAAAGGTTTGTGGTGAGAAAGTCATGCAATCCAGCATTTGCTGCTGCACAATTGAAATTTGAAGAGCTGAGTACAAATTCGACAGTCAGCAGGTCCAGCAGCTTGTCACATCTGGATGGGGCGAGCAAGTCAAGGGTTCACGCTCCTAGTTCGCAAGAAGATTGCTCCTCAAAGCAAGATGGCACAGGCATGCCAGATAGATCGGTTGGTCATGATACTAAAATGATTATTGCTGCATCAGAATGTGGGACTGAGATATCAATTTCATCCACACTGGACTCACCAGATAGATCAGAAGGTGACGGTGGGGAGATCGTGTTGGAGATTGGAGCTCTGGAAAATAGAAACTATGTCACCAGCAAAGCTGATAATGACTTCAATACTGTCCATCCCGAGGTAAAAAATGCTCCTGAAGTAGAAGCCCAGCCGCAGAAGCAAATAGAACAGAACGGCCATGTTCCTGCCCTCGAAATAGAAGCCCAGCCACAAGACGAGCTTGTTCAGGAACCGCATGCTGAACCGGAAATGCCAGGTTTGCATGAACGATTGGAAAAACCTGTCGCATCTTACGCCACACCTGAAGGAACTCCAATGAGCCGAACGACTATCACTGAATCTCAAGAAACACCGTCAAGTGAGGTCTCTGTTAATACCAAAAAGAGCAGGAGCAAAAAGCCCAAGCCCCATGCAAGCAAGAGGTCGCTGGCTAGTCCTGGCAGCGACTCAGTCGGCCGGAGCAGCACCGACAACTTCTCAAAGGAATCAAGGCATCCCAAGAGAGAGAGCTCAAGCAAGGCTGCTAAATCCGACCACGTCGAACAAGAACCTCGCACGAGCAACAGCAACAGCAACCTGTTGCCAAGCTACATGCAATTTACAGAATCTGCAAGAGCCAAGGCGTCGTCGTCCCCGAAGATGAGCCCAGATGTGCAGGACAGCAACCCAAGGAAAAGGCACTCGTTGCCCATGACCGACGGCAAGCATGACTCGTCGCCTCGCATGCAGCGATCGTCGTCACATGCTCAGCAAAACGTCAAGAGCAACGGGCCTGCTCCACACAGCGCGTCTGACAGGAAGTGGCATATC