| TFome pUT6637 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728355 |
| Protein Name | ZmbZIP128 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM2G016150 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6637
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0012_F |
| Temperature (ºC) | 72.3 |
| Sequence
|
caccATGGAGCGCGTCTTCTCCGTGG |
3' Primer
| Name | CD0012_R |
| Temperature (ºC) | |
| Sequence
|
GGAGGTCGACCCCGATGAAGCC |
PCR Condition
PCR Conditions: per reaction (25l): 10 l GC buffer
Amino Acid Sequence
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MERVFSVEEIPNPYWVPPHPQSAAAGAVAAPAGEAAGLMNRCPSEWYFQKFLEEAVLDSPVPVAGVSRGSVGAGVEAAERKTPGTAAAAAASSSVVDPVEYNAMLKQKLEKDLAAVALWRASGAAPPDNSPAGSSLPSVDVPHAGPLKPMGGTGSLVQNKLAGAPGGGSSPHVVQNADIPVKQTTSSSSREQSDDDDMEGDAETTGNGNPVQQRLQRRKQSNRESARRSRSRKAAHLNELEAQVAQLRVENSSLLRRLADVNQKFNEAAVDNRVLKADVETLRAKVKMAEDSVKRVTGMNTLFPAVSDMSSLSMPFNGSPSDSASDAAVPIQDDLNSYFANPSEIGGSNGYMPDIASSAQEDDDFVNGAQVAGKMGSTDSLQRVASLEHLQKRMCGGPASSGSTS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGCGCGTCTTCTCCGTGGAGGAGATCCCCAACCCCTACTGGGTTCCGCCGCACCCTCAATCGGCGGCCGCCGGCGCTGTTGCTGCACCAGCGGGGGAGGCGGCGGGGCTGATGAACCGGTGCCCGTCGGAATGGTACTTCCAGAAGTTCCTGGAGGAGGCCGTGCTCGACAGCCCGGTTCCCGTAGCCGGCGTCAGTAGGGGCAGCGTTGGAGCTGGAGTTGAGGCTGCGGAGAGGAAGACACCGGGGACCGCGGCGGCGGCGGCTGCCTCGAGCTCGGTTGTTGACCCCGTGGAGTACAACGCGATGCTCAAGCAGAAGCTGGAGAAGGACCTCGCTGCCGTTGCCTTGTGGAGGGCTTCTGGTGCAGCACCTCCAGATAATTCTCCAGCTGGTTCATCCTTGCCAAGTGTGGATGTTCCACATGCAGGCCCTCTTAAACCCATGGGAGGTACTGGAAGTCTAGTTCAAAACAAGCTAGCTGGTGCTCCAGGCGGGGGATCAAGTCCACATGTAGTACAAAATGCCGATATTCCTGTTAAGCAAACCACTAGCTCTTCCTCACGTGAGCAGTCAGACGATGATGATATGGAAGGAGATGCTGAGACTACTGGAAACGGAAACCCTGTTCAACAAAGATTACAGAGAAGGAAGCAATCCAACCGAGAATCAGCCAGGCGTTCGAGAAGCAGAAAGGCAGCTCACTTGAATGAACTGGAGGCACAGGTAGCACAGTTAAGAGTTGAGAACTCTTCGCTGCTAAGGCGACTTGCTGACGTTAATCAGAAGTTCAATGAGGCTGCTGTTGACAATAGGGTGCTAAAGGCAGATGTCGAAACCTTAAGAGCAAAGGTGAAGATGGCAGAGGACTCAGTGAAGCGGGTAACTGGCATGAACACATTGTTCCCTGCCGTGTCTGATATGTCGTCCCTCAGCATGCCATTCAATGGCTCCCCATCCGACTCCGCCTCTGATGCCGCCGTACCCATCCAAGATGACCTGAACAGTTACTTCGCCAATCCAAGCGAGATCGGAGGCAGCAACGGTTACATGCCAGATATAGCTTCCTCGGCTCAAGAGGACGACGATTTCGTCAACGGGGCTCAGGTCGCCGGCAAGATGGGCAGCACTGACTCGCTGCAGCGGGTGGCGAGCCTGGAGCACCTCCAGAAGAGGATGTGCGGAGGCCCGGCTTCATCGGGGTCGACCTCC
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