| TFome pUT6640 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728358 |
| Protein Name | ZmCA2P13 |
| Species | Zea mays |
| TF Family | CCAAT-HAP2 |
| Gene id | GRMZM5G857944 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6640
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0408_F |
| Temperature (ºC) | 67.4 |
| Sequence
|
caccATGCTTCTTCCCTCTTCGTCTTC |
3' Primer
| Name | CD0408_R |
| Temperature (ºC) | |
| Sequence
|
TCTCATAACTGGAACCCTATGCTG |
PCR Condition
5 l GC buffer (Thermo Fisher) 0.6 l primer forward
Amino Acid Sequence
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MLLPSSSSSSASASKGNSFGKTVNDHLRSTLSFDNKQPPFASQNFDYGQTIACISYPYNRSRSGDVWAAYESRTSTATVFRSQIAGGGSSTRIPLPLELAENEPIYVNPKQYHGILRRRQLRAKLEAQNKLVRARKPYLHESRHLHAMKRARGSGGRFLNTKQLQQSHTALTRSTTTSGTSSSGSTHLRLGGGAAAAGDRSVLAPKTMASQDSSKKAVSSALAFTATPMLRRDDGFLQHPSHLFSFSGHFGQASAQAGVHNGSQHRVPVMR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCTTCTTCCCTCTTCGTCTTCGTCTTCCGCTTCCGCTTCCAAAGGTAACTCCTTTGGGAAAACCGTTAACGATCATCTGAGGTCAACTTTGAGTTTTGATAACAAGCAACCTCCATTTGCGAGTCAAAACTTTGACTACGGTCAAACAATAGCTTGCATTTCATACCCGTACAATCGTTCTAGATCAGGAGATGTTTGGGCAGCCTATGAGTCACGCACCAGCACTGCCACTGTGTTCCGTTCCCAAATTGCTGGTGGGGGTTCATCCACAAGAATTCCCTTGCCTTTGGAATTAGCAGAGAATGAACCCATATATGTGAATCCCAAACAATATCACGGGATACTTCGCAGAAGACAGTTACGTGCCAAGCTAGAGGCTCAGAACAAGCTAGTCAGAGCCCGAAAGCCTTACCTTCATGAGTCTAGGCATCTTCATGCAATGAAGAGGGCACGAGGTTCCGGTGGACGATTCCTCAACACTAAGCAGCTCCAGCAGTCTCACACAGCCCTCACCAGGTCCACCACCACAAGTGGCACAAGCTCCTCAGGCTCAACTCATCTGCGGCTTGGTGGTGGCGCAGCCGCAGCTGGAGATCGATCTGTGCTGGCACCCAAAACAATGGCCTCACAAGACAGTAGCAAGAAGGCCGTTTCTTCAGCCCTCGCCTTCACTGCGACTCCAATGCTGCGCAGAGATGACGGCTTCTTGCAGCACCCAAGCCATCTTTTCAGTTTTTCTGGTCATTTTGGGCAGGCAAGCGCGCAAGCTGGCGTCCATAATGGAAGTCAGCATAGGGTTCCAGTTATGAGA
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