| TFome pUT6641 | |
|---|---|
| Template | BT066369 |
| Genbank Insert ID | KJ728359 |
| Protein Name | ZmOrphan221 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G089010 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6641
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C495_F |
| Temperature (ºC) | 68.8 |
| Sequence
|
caccATGGTGGTGGGAACGGC |
3' Primer
| Name | C495_R |
| Temperature (ºC) | |
| Sequence
|
ACTGTTCTGAAGGACCCTATACAGCTCA |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MVVGTAPCGVSVSSVWILLLLSSLLLSPSAASVDFGHCGCDDADDGALSSTYNILQCQKFGAFIVLCGLTHLITVFTYDPHSFHLVLALTVAKFMTALVSFATAITLLTLIPQLLRVKVRENFLVNKARELDREVGMMKMKEEASWHVRMLTQEIRKSLDRHTILYTTMVELSKALELQNCAVWMPDETRSEMILTHQPRERDIMDQQNCSIPIDDPDVQEIKATKDAKVLGPDSALGVATRKLDVGPVAAIRMPMLRVSNFKGGTPEVMQTSYAILVLVLPNDGSLGWGRRELEIVEVVADQVAVALSHAALLEESQLMREKLAEQYRDLLQAKHEAMRAGEARNSFQTAMYDGMRRPMHSILGLVSMMQQESMNPEQRVVMDAIAKTSSVASTLMNDVMQTSTMNCEHLSLVRRPFNLHSFIKEAVGVVRCLTGCKGVEFEFQVDNSLPERIIGDEKRVFHIVLHMVGTLINRCNVGCISLYVNGHNEVEERHNHDWMLRRTNFSGGYVCVKFEIRIRKSKDYLLSSNGQISHGSKPNNSEMGLSFNMCKKIVQMMNGNIWSVSDSKSVGETIMLVLQFQLQPLTAVSSAASSDLSRSSAIPNFNGLRVLLADSDDTNRAVTHRLLEKLGCRVLSVASGVQCTSSFAAEPSFQLVVLDLALQRTDGLEVARAIRKFSSNSWLPLIVALAARIDDKVRDGCQRSGISGLIQKPATLAALGDELYRVLQNS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGTGGTGGGAACGGCGCCGTGCGGGGTCTCCGTCTCCTCCGTGTGGATCCTCCTGCTCCTTTCCTCCCTGCTCCTCTCGCCGTCGGCGGCGTCCGTCGATTTCGGCCACTGCGGCTGCGACGACGCCGACGACGGCGCCCTCTCGAGCACCTACAACATCCTGCAATGCCAGAAGTTCGGCGCCTTCATCGTGCTCTGCGGCCTCACGCACCTCATCACCGTGTTCACCTACGACCCGCACTCCTTCCACCTCGTGCTCGCCCTCACCGTCGCCAAGTTCATGACGGCACTAGTCTCCTTCGCCACAGCCATCACGCTGCTGACACTGATACCGCAGCTCCTGAGGGTGAAGGTCAGGGAAAACTTCCTGGTGAACAAGGCACGTGAGCTGGACCGGGAGGTGGGGATGATGAAAATGAAAGAAGAGGCGAGCTGGCATGTGCGTATGCTCACACAGGAGATCCGCAAGTCGCTCGACAGGCACACCATCTTGTACACCACCATGGTTGAGCTCTCGAAAGCGCTGGAACTGCAGAATTGTGCTGTCTGGATGCCCGATGAAACCAGGAGCGAGATGATCTTAACTCATCAGCCAAGGGAAAGGGATATAATGGACCAGCAGAACTGCTCGATTCCTATTGATGATCCAGATGTTCAAGAAATAAAGGCTACCAAGGACGCAAAAGTTCTTGGGCCAGATTCGGCACTAGGGGTTGCTACCCGCAAGCTTGACGTGGGGCCTGTGGCTGCAATAAGGATGCCGATGTTAAGGGTGTCAAATTTCAAAGGAGGGACTCCAGAAGTGATGCAGACGAGCTATGCTATCTTGGTTCTGGTTTTGCCTAATGATGGTTCATTGGGGTGGGGTAGAAGAGAGTTGGAGATTGTTGAAGTAGTTGCTGACCAAGTTGCGGTCGCTTTGTCACATGCTGCACTCCTAGAGGAGTCTCAGCTGATGCGAGAGAAACTTGCTGAGCAGTATAGGGACTTGCTGCAGGCAAAGCATGAAGCCATGAGGGCAGGGGAAGCTCGGAATTCCTTCCAGACTGCAATGTACGACGGAATGCGAAGGCCAATGCACTCAATCCTTGGTCTTGTCTCAATGATGCAACAGGAGAGCATGAATCCAGAGCAAAGGGTTGTGATGGATGCCATTGCCAAGACAAGCAGTGTTGCGTCCACACTGATGAATGATGTGATGCAAACATCGACAATGAACTGTGAGCACTTGTCTTTGGTGAGGAGGCCGTTCAATCTTCATTCTTTTATTAAAGAAGCTGTTGGAGTGGTCAGATGTCTAACTGGTTGCAAGGGTGTAGAGTTTGAGTTTCAAGTGGATAATTCTTTGCCAGAAAGGATCATTGGTGATGAGAAGAGAGTCTTCCACATTGTCCTGCACATGGTAGGCACCCTAATAAACCGATGTAATGTCGGCTGTATCTCGTTATATGTCAATGGTCATAATGAGGTTGAAGAGAGGCATAATCATGACTGGATGCTGCGGAGAACAAACTTCTCTGGGGGCTATGTTTGTGTGAAATTTGAGATTAGGATTAGAAAATCCAAGGACTATCTTTTGAGTTCAAACGGTCAGATAAGTCATGGGTCCAAACCAAACAATTCTGAGATGGGGCTTAGCTTCAATATGTGCAAGAAGATTGTGCAGATGATGAACGGCAACATTTGGTCAGTATCAGATTCTAAAAGCGTTGGAGAAACCATCATGCTGGTCCTCCAGTTCCAGCTGCAGCCTCTGACTGCGGTCTCCTCCGCGGCGTCTTCAGACTTGAGCCGATCGTCCGCAATCCCCAACTTCAACGGGCTCAGAGTCCTCCTGGCGGACAGCGACGACACCAACAGAGCAGTAACACACAGGCTCCTGGAGAAGCTCGGCTGCCGGGTCCTTTCGGTCGCCTCCGGTGTCCAATGCACGAGCTCCTTCGCCGCCGAGCCGTCCTTCCAGCTGGTGGTCCTGGACCTCGCCTTGCAGAGGACGGACGGGCTCGAAGTGGCCCGCGCGATCAGGAAGTTCAGTAGCAATAGCTGGCTGCCGCTGATCGTCGCCCTAGCTGCGAGGATCGATGACAAGGTCCGAGACGGATGCCAGAGGTCGGGGATAAGCGGCCTGATCCAGAAACCGGCCACGTTAGCTGCGCTGGGAGATGAGCTGTATAGGGTCCTTCAGAACAGT
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