| TFome pUT6644 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728362 |
| Protein Name | ZmHB53 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G044752 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6644
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0076_F |
| Temperature (ºC) | 76.1 |
| Sequence
|
caccATGGAGCGCGGCGACTGCCAGTTCAC |
3' Primer
| Name | CD0076_R |
| Temperature (ºC) | |
| Sequence
|
GGCCACCGCGTTCCACTCCAGCAGC |
PCR Condition
5x GC Buffer 4 ul 10mM dNTP 0.4 ul 20mM Primer F 0
Amino Acid Sequence
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MMERVEDLGLSLSLSSSLASPRTHHVATMLLRAPEKRFLEMPLLLPAKRTTEVTGEDGLRGGSDEEDGGCGIDGSRKKLRLSKDQSAVLEDSFREHPTLNPRQKAALAQQLGLRPRQVEVWFQNRRARTKLKQTEVDCEYLKRCCETLTEENRRLQKEVQELRALKLVSPHLYMHMSPPTTLTMCPSCERVSSSNGNSAAATAAARARAGAGAGAIVCHPIDRATST
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGGAGAGGGTCGAGGACTTAGGGCTCAGCCTCAGCCTCAGCTCGTCCCTCGCGTCTCCTCGCACTCACCATGTCGCCACCATGCTGCTACGCGCTCCAGAGAAGAGGTTCCTGGAGATGCCACTGCTGCTGCCCGCGAAGCGGACGACCGAGGTCACCGGCGAGGATGGCCTGCGAGGCGGCAGCGATGAGGAGGACGGCGGCTGCGGCATCGACGGCTCCAGGAAGAAGCTCCGGCTGTCCAAGGACCAGTCCGCGGTGCTCGAGGATAGCTTCCGGGAGCACCCAACTCTCAACCCTCGGCAGAAGGCAGCCTTGGCGCAGCAGCTAGGCCTGCGGCCCCGCCAGGTGGAGGTGTGGTTCCAGAACAGGCGCGCCAGGACGAAGCTGAAGCAGACGGAGGTGGACTGCGAGTACCTGAAGCGCTGCTGCGAGACGCTGACGGAGGAGAACCGGCGGCTGCAGAAGGAGGTGCAGGAGCTCCGCGCGCTCAAGCTCGTGTCGCCGCACCTCTACATGCACATGTCCCCGCCCACCACCCTCACCATGTGCCCCTCCTGCGAGCGCGTCTCCTCGTCCAACGGCAACTCCGCAGCTGCCACGGCGGCCGCGCGCGCGCGCGCCGGCGCCGGCGCCGGCGCCATCGTCTGCCACCCGATCGACCGAGCCACTAGTACG
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