| TFome pUT6660 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728378 |
| Protein Name | ZmOrphan1 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G111232 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G124T p.A42S c.A141G p.S47(=) PZE0210955342; c.A145G p.T49A. c.A177G p.S59(=) c.G230A p.G77D c.A261G p.K87(=) PZE0210955462; c.C273T p.N91(=) c.C291T p.F97(=) c.C297T p. I99(=) PZE0210955498; c.C334T p.L112(=) c.C351T p.F117(=) c.T387C p.S129(=) c.G391C p.E131Q SNPs all present in BT084381, though that FlcDNA only matches this cloned sequence 1-700bp. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6660
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0365_F |
| Temperature (ºC) | 69.3 |
| Sequence
|
caccATGGTCGGCCGGATGGA |
3' Primer
| Name | CD0365_R |
| Temperature (ºC) | |
| Sequence
|
GCAGCGGAAACGGATGACAAT |
PCR Condition
PCR Reaction Mix:5 l GC buffer (Thermo Fisher) 0.6
Amino Acid Sequence
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MVGRMERQTASSSASCSSSSSSSSVCGGKKRPDILNMIRNASCLNSSSADTGKGRSKLSTNKVTHGFHLVEGKSGHDMEDYHVAEYKYVKNHELGLFAIFDGHLGDKVPSYLKANLFSNIMKEPLFWSSPQEAIKNAYCSTNKYILENGKQLGPGGSTAVTAIVVDGKDMLIANVGDSRAVVCEMGSANQLTVDHEPDTTEERQRIEKHGGFVTTFPGDVPRVNGQLAVARAFGDQSLKAHLSSKPDTRHVPIDSSIEFVILASDGLWKVMKNQEAVDLVKSIKDPKAAAKRLTTEALARKSKDDISCIVIRFRC
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGTCGGCCGGATGGAGCGGCAGACCGCGTCGTCGTCAGCTTCGTGCTCCTCCTCCTCCTCTTCCTCCTCGGTCTGCGGCGGCAAGAAGCGCCCTGATATCCTCAACATGATCCGGAATGCATCCTGTCTTAATTCGTCGTCTGCTGATACCGGCAAGGGAAGGAGTAAATTGTCGACCAACAAAGTTACACATGGATTCCACCTGGTGGAAGGCAAGTCCGGCCATGACATGGAAGACTACCATGTGGCAGAGTACAAGTATGTGAAGAATCATGAGCTTGGTCTCTTTGCCATTTTTGATGGCCATTTAGGAGATAAGGTGCCAAGTTACTTGAAAGCTAACCTTTTTAGCAACATAATGAAAGAGCCTCTCTTCTGGTCTAGCCCTCAAGAAGCAATAAAGAATGCATACTGCTCTACAAACAAATATATCCTGGAAAATGGCAAACAACTTGGACCAGGTGGTTCAACAGCAGTTACAGCTATTGTAGTCGATGGTAAGGACATGTTGATCGCAAATGTAGGCGACTCTCGAGCTGTTGTGTGTGAAATGGGCTCTGCTAATCAGCTCACTGTTGACCATGAGCCCGATACAACTGAGGAAAGGCAGAGGATTGAAAAGCACGGTGGCTTTGTAACGACATTTCCTGGTGATGTTCCTCGGGTGAATGGCCAGCTTGCTGTTGCTAGAGCATTTGGTGACCAAAGCCTCAAGGCCCACTTGAGCTCTAAACCTGATACTAGACATGTACCAATAGACTCAAGTATAGAGTTCGTCATACTTGCCAGTGATGGATTGTGGAAGGTAATGAAGAACCAGGAAGCCGTGGATCTCGTGAAGTCAATCAAGGATCCCAAGGCAGCAGCGAAGCGATTGACCACTGAAGCGTTGGCAAGGAAGAGCAAAGATGACATCTCTTGCATTGTCATCCGTTTCCGCTGC
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