| TFome pUT6665 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ728382 |
| Protein Name | ZmEREB199 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G163745 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T44Ap.V15E; c.T48Gp. S16(=); c.G231Ap. G77(=) Not in functional domain. c.T48G;G231A p.(=) present in maize HapMap2: p. 15 yes PZE01208182991; c.48 yes PZE01208182995; c. 231 yes PZE01208183178 |
| Request Information | Deposited to ABRC |
TFome clone pUT6665
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE0065_F |
| Temperature (ºC) | 65.8 |
| Sequence
|
caccATGGACGATGGCGCCGG |
3' Primer
| Name | SE0065_R |
| Temperature (ºC) | |
| Sequence
|
GAATCGCCAGAGGTCGTCTGAGATGAAT |
PCR Condition
5 l GC buffer 1.5l MgCl2 50mM 1.25 l DMSO 100% 1 l
Amino Acid Sequence
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MDDGAGASRSDDGEESPRLPAERRYKGVRLRKWGRWVSEIRMPNSRERVWLGSYESAEKAARAFDAAAVCLRGSRAGSLNFPESPPNVRYVPGALLTPEQIQAEAARHANQQLPSRPVASALQPAAAPPAGGPSSDRTTLSMPSTDYSSGAAVCGDDEALDWSFMDALPPMPPASSSVGMGNSAHTVPAAMDDFMYGSPHLVVQPSEDATQDVIDSDDDHTFISDDLWRF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACGATGGCGCCGGAGCAAGCCGCAGCGACGACGGCGAGGAATCGCCGCGGCTGCCGGCGGAGCGGCGTTACAAGGGCGTGCGCCTGCGGAAATGGGGCCGGTGGGTGTCGGAGATCCGGATGCCCAACAGCCGCGAGAGGGTCTGGTTGGGCTCATACGAGTCAGCGGAGAAGGCGGCGCGGGCGTTCGACGCCGCTGCCGTCTGCCTCCGCGGCTCCCGGGCCGGATCGCTCAACTTCCCCGAGTCTCCGCCCAACGTCCGGTACGTCCCTGGCGCGCTGCTGACGCCTGAGCAGATTCAGGCAGAAGCTGCAAGGCACGCTAACCAGCAGCTGCCTAGTCGGCCGGTCGCGTCGGCGTTGCAGCCAGCAGCTGCACCACCCGCGGGAGGACCAAGCAGTGACAGGACGACCTTGTCCATGCCGTCCACTGACTACAGTAGTGGAGCTGCCGTCTGCGGTGACGACGAGGCGCTTGATTGGTCTTTCATGGATGCTTTGCCACCTATGCCGCCGGCGTCTTCATCAGTGGGGATGGGGAACAGTGCTCATACTGTACCGGCGGCCATGGATGATTTCATGTACGGGTCCCCTCACCTGGTTGTACAGCCGAGTGAAGATGCCACACAGGATGTAATTGACAGCGATGATGACCATACATTCATCTCAGACGACCTCTGGCGATTC
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