TFome clone pUT6667

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B501_F
Temperature (ºC)	71.5
Sequence Copy to clipboard	caccATGGAGACGCCACAGCAGCAA

3' Primer

Name	B501_R
Temperature (ºC)
Sequence Copy to clipboard	GCGGCCTCCGGAGGACTGGA

PCR Condition

5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr

Amino Acid Sequence

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METPQQQSAAAAAAAAHGQDDGGSPPMSPASAAAAALANARWNPTKEQVAVLEGLYEHGLRTPSAEQIQQITGRLREHGAIEGKNVFYWFQNHKARQRQRQKQDSFAYFSRLLRRPPPLPVLSMPPAPPYHHARVPAPPAIPMPMAPPPPAACNDNGGARVIYRNPFYVAAPQAPPANAAYYYPQPQQQQQQQVTVMYQYPRMEVAGQDKMMTRAAAHQQQQHNGAGQQPGRAGHPSRETLQLFPLQPTFVLRHDKGRAANGSNNDSLTSTSTATATATATATASASISEDSDGLESGSSGKGVEEAPALPFYDFFGLQSSGGR

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGAGACGCCACAGCAGCAATCCGCCGCCGCCGCCGCCGCCGCCGCCCACGGGCAGGACGACGGCGGGTCGCCGCCGATGTCGCCGGCCTCCGCCGCGGCGGCGGCGCTGGCGAACGCGCGGTGGAACCCGACCAAGGAGCAGGTGGCCGTGCTGGAGGGGCTGTACGAGCACGGCCTGCGCACCCCCAGCGCGGAGCAGATACAGCAGATCACGGGCAGGCTGCGGGAGCACGGCGCCATCGAGGGCAAGAACGTCTTCTACTGGTTCCAGAACCACAAGGCCCGCCAGCGCCAGAGGCAGAAGCAGGACAGCTTCGCCTACTTCAGCAGGCTCCTCCGCCGGCCCCCGCCGCTGCCCGTGCTCTCCATGCCCCCCGCGCCACCGTACCATCACGCCCGCGTCCCGGCGCCGCCCGCGATACCGATGCCGATGGCGCCGCCGCCGCCCGCTGCATGCAACGACAACGGCGGCGCGCGTGTGATCTACAGGAACCCATTCTACGTGGCTGCGCCGCAGGCGCCCCCTGCAAATGCCGCCTACTACTACCCACAGCCACAGCAGCAGCAGCAGCAGCAGGTGACAGTCATGTACCAGTACCCGAGAATGGAGGTAGCCGGCCAGGACAAGATGATGACCAGGGCCGCGGCGCACCAGCAGCAGCAGCACAACGGCGCCGGGCAACAACCGGGACGCGCCGGCCACCCCAGCCGCGAGACGCTCCAGCTGTTCCCGCTCCAGCCCACCTTCGTGCTGCGGCACGACAAGGGGCGCGCCGCCAACGGCAGTAATAACGACTCCCTGACGTCGACGTCGACGGCGACTGCGACAGCGACAGCGACAGCGACAGCGTCCGCTTCCATCTCCGAGGACTCGGATGGCCTGGAGAGCGGCAGCTCCGGCAAGGGCGTCGAGGAGGCGCCCGCGCTGCCGTTCTATGACTTCTTCGGGCTCCAGTCCTCCGGAGGCCGC